An improved procedure for Percoll gradient separation of sporogonial stages in Encephalitozoon cuniculi (Microsporidia)

Parasitology Research

Volumn 99, Issue 6, 2006, Pages 708-714

  Taupin, Vanessa ^a   Méténier, Guy ^a   Vivarès, Christian P ^a   Prensier, Gérard ^a  

^a UNIVERSITÉ CLERMONT AUVERGNE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHATE BUFFERED SALINE; SAPONIN; TRITON X 100;

ARTICLE; CELL DIFFERENTIATION; CELL SEPARATION; DNA FINGERPRINTING; ENCEPHALITOZOON CUNICULI; FRACTIONATION; GLASS BOTTLE; HOST CELL; KIDNEY CELL CULTURE; MAMMAL CELL; MICROSPORIDA; NONHUMAN; PARASITE IDENTIFICATION; PARASITOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; PROTOZOAN SPORE; SEPARATION TECHNIQUE; SPOROGENESIS; SUCROSE DENSITY GRADIENT CENTRIFUGATION; SUPERNATANT; TRANSMISSION ELECTRON MICROSCOPY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; CELL LINE; CENTRIFUGATION, DENSITY GRADIENT; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENCEPHALITOZOON CUNICULI; FUNGAL PROTEINS; MICROSCOPY, ELECTRON, TRANSMISSION; MYCOLOGY; SPORES, FUNGAL;

ENCEPHALITOZOON CUNICULI; MAMMALIA; MICROSPORIDIA;

EID: 33749428529     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-006-0231-y     Document Type: Article

References (23)

1. Bacchi CJ, Lane S, Weiss LM, Yarlett N, Takvorian P, Cali A, Wittner M (2001) Polyamine synthesis and interconversion by the Microsporidian Encephalitozoon cuniculi. J Eukaryot Microbiol 48:374-381
2. Bacchi CJ, Rattendi D, Faciane E, Yarlett N, Weiss LM, Frydman B, Woster P, Wei B, Marton LJ, Wittner M (2004) Polyamine metabolism in a member of the phylum Microspora (Encephalitozoon cuniculi): effects of polyamine analogues. Microbiology 150:1215-1224
3. Beauvais B, Sarfati C, Challier S, Derouin F (1994) In vitro model to assess effect of antimicrobial agents on Encephalitozoon cuniculi. Antimicrob Agents Chemother 38:2440-2448
4. Bente M, Harder S, Wiesgigl M, Heukeshoven J, Gelhaus C, Krause E, Clos J, Bruchhaus I (2003) Developmentally induced changes of the proteome in the protozoan parasite Leishmania donovani. Proteomics 3:1811-1829
5. Bigliardi E, Riparbelli MG, Selmi MG, Bini L, Liberatori S, Pallini V, Bernuzzi A, Gatti S, Scaglia M, Sacchi L (1999) Evidence of actin in the cytoskeleton of microsporidia. J Eukaryot Microbiol 46:410-415
6. Böhne W, Ferguson DJ, Kohler K, Gross U (2000) Developmental expression of a tandemly repeated, glycine- and serine-rich spore wall protein in the microsporidian pathogen Encephalitozoon cuniculi. Infect Immun 68:2268-2275
7. Brosson D, Kuhn L, Delbac F, Garin J, Vivarès C, Texier C (2006) Proteomic analysis of the eucaryotic parasite Encephalitozoon cuniculi (Microsporidia): a reference map for proteins expressed in late sporogonial stages. Proteomics vol 6 (in press) DOI: 10.1002/pmic.200500796
8. Chavant P, Taupin V, El Alaoui H, Wawrzyniak I, Chambon C, Prensier G, Méténier G, Vivarès CP (2005) Proteolytic activity in Encephalitozoon cuniculi sporogonial stages: predominance of metallopeptidases including an aminopeptidase-P-like enzyme. Int J Parasitol 35:1425-1433
9. Delbac F, Peyret P, Méténier G, David D, Danchin A, Vivarès CP (1998) On proteins of the microsporidian invasive apparatus: complete sequence of a polar tube protein of Encephalitozoon cuniculi. Mol Microbiol 29:825-834
10. Didier ES (2005) Microsporidiosis: an emerging and opportunistic infection in humans and animals. Acta Trop 94:61-76
11. Fasshauer V, Gross U, Bohne W (2005) The parasitophorous vacuole membrane of Encephalitozoon cuniculi lacks host cell membrane proteins immediately after invasion. Eukaryot Cell 4:221-224
12. Florens L, Washburn MP, Raine JD, Anthony RM, Grainger M, Haynes JD, Moch JK, Muster N, Sacci JB, Tabb DL, Witney AA, Wolters D, Wu Y, Gardner MJ, Holder AA, Sinden RE, Yates JR, Carucci DJ (2002) A proteomic view of the Plasmodium falciparum life cycle. Nature 419:520-526
13. Franzen C, Müller A (2001) Microsporidiosis: human diseases and diagnosis. Microbes Infect 3:389-400
14. Green LC, Didier PJ, Didier ES (1999) Fractionation of sporogonial stages of the microsporidian Encephalitozoon cuniculi by Percoll gradients. J Eukaryot Microbiol 46:434-438
15. Katinka MD, Duprat S, Cornillot E, Méténier G, Thomarat F, Prensier G, Barbe V, Peyretaillade E, Brottier P, Wincker P, Delbac F, El Alaoui H, Peyret P, Saurin W, Gouy M, Weissenbach J, Vivarès CP (2001) Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi. Nature 414:450-453
16. Kima PE, Dunn W (2005) Exploiting calnexin expression on phagosomes to isolate Leishmania parasitophorous vacuoles. Microb Pathog 38:139-145
17. Pakes SP, Shadduck JA, Cali A (1975) Fine structure of Encephalitozoon cuniculi from rabbits, mice and hamsters. J Protozool 22:481-488
18. Peek R, Delbac F, Speijer D, Polonais V, Greve S, Wentink-Bonnema E, Ringrose J, van Gool T (2005) Carbohydrate moieties of microsporidian polar tube proteins are targeted by immunoglobulin G in immunocompetent individuals. Infect Immun 73:7906-7913
19. Peuvel I, Peyret P, Méténier G, Vivarès CP, Delbac F (2002) The microsporidian polar tube: evidence for a third polar tube protein (PTP3) in Encephalitozoon cuniculi. Mol Biochem Parasitol 122:69-80
20. Seleznev KV, Issi IV, Dolgikh VV, Belostotskaya GB, Antova OA, Sokolova JJ (1995) Fractionation of different life cycle stages of microsporidia Nosema grylli from crickets Gryllus bimaculatus by centrifugation in Percoll density gradient for biochemical research. J Eukaryot Microbiol 42:288-292
21. Taupin V, Méténier G, Delbac F, Vivarès CP, Prensier G (2006) Expression of two cell wall proteins during the intracellular development of Encephalitozoon cuniculi: an immunocytochemical and in situ hybridization study with ultrathin frozen sections. Parasitology Jun 132(Pt 6):815-825 DOI: 10.1017/S0031182005009777
22. Vavra J, Larson J (1999) Structure of the microsporidia. In: Wittner & Weiss (eds) The microsporidia and microsporidiosis. ASM, Washington, DC, pp 7-84
23. Xu Y, Takvorian PM, Cali A, Orr G, Weiss LM (2004) Glycosylation of the major polar tube protein of Encephalitozoon hellem, a microsporidian parasite that infects humans. Infect Immun 72:6341-6350