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Volumn 74, Issue 3, 2006, Pages

Coexistence of tonic firing and bursting in cortical neurons

Author keywords

[No Author keywords available]

Indexed keywords

BRAIN; HYSTERESIS; MATHEMATICAL MODELS; OSCILLATIONS; POTASSIUM;

EID: 33749417567     PISSN: 15393755     EISSN: 15502376     Source Type: Journal    
DOI: 10.1103/PhysRevE.74.031922     Document Type: Article
Times cited : (90)

References (35)
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    • JNMEDT 0165-0270
    • Transverse hippocampal slices (400 μm) from a male Wistar rat (p18) were cut in ice cold standard artificial cerebrospinal fluid (ACSF) equilibrated with 95% O2 and 5% C O2 (119 mM NaCl, 2.5 mM KCl, 1.3 mM NaHP O4, 1.3 mM Mg Cl2, 2.5 mM Ca Cl2, 26 mM NaHC O3, and 11 mM glucose) and then transferred into an interface chamber where they were incubated at 34 °C for 45 minutes. A multiwire electrode (ALA Scientific, Westbury, NY) was used for recording spontaneous spiking activity in CA3. Fast synaptic transmission was blocked by bath application of 10 μM NBQX and 2.5 μM Gabazine (Tocris Bioscience, Ellisville, MO). Slices were submerged in standard ACSF (32 °C) ; the high potassium condition corresponded to an increase of KCl concentration to 6.5 mM. Extracellular traces were band pass filtered (300 Hz, 5000 kHz) and sampled at 20 kHz. Potential spikes were detected by threshold crossing. A spike from a given unit was typically picked up by 3-4 recording sites (tetrodelike recordings). Spikes were then sorted by first overclustering the wave forms into subclusters with the k -means clustering algorithm. As a result, spikes corresponding to a single unit were represented by several neighboring subclusters. These subclusters were then manually combined to clusters representing individual units. Details in M. S. Fee, P. P. Mitra, and D. Kleinfeld, J. Neurosci. Methods JNMEDT 0165-0270 69, 175 (1996). All experiments were carried out in accordance with the guidelines set forth by the University of California.
    • (1996) J. Neurosci. Methods , vol.69 , pp. 175
    • Fee, M.S.1    Mitra, P.P.2    Kleinfeld, D.3
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    • edited by C. Koch and I. Segev (MIT Press, Cambridge, Massachusetts
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.