Effectiveness of genotype-based selection in the production of marker-free and genetically fixed transgenic lineages: Ectopic expression of a pistil chitinase gene increases leaf-chitinase activity in transgenic rice plants without hygromycin-resistance gene

Plant Biotechnology

Volumn 23, Issue 4, 2006, Pages 349-356

  Hashizume, Fujio ^a,b   Nakazaki, Tetsuya ^c   Tsuchiya, Tohru ^d   Matsuda, Tsukasa ^b  

^a Science and Technology Promotion Center   (Japan)

^b NAGOYA UNIVERSITY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

^d MIE UNIVERSITY   (Japan)

Author keywords

Genetically modified crops; Marker free; One factor inheritance; Oryza sativa cv. Koshihikari; Pathogenesis related protein

Indexed keywords

FUNGI; MAGNAPORTHE GRISEA; ORYZA SATIVA;

EID: 33749375454     PISSN: 13424580     EISSN: 13476114     Source Type: Journal    
DOI: 10.5511/plantbiotechnology.23.349     Document Type: Article

References (25)

1. Asaga K (1981) A procedure for evaluating field resistance to blast in rice varieties. J. of the Central Agricultural Experiment (Nougishikenhou) 35: 51-138 (in Japanease)
2. Broglie KE, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais CJ, Broglie R (1991) Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254: 1194-1197
3. Christou P, Ford TL, Kofron M (1991) Production of transgenic rice (Oryza sativa L.) plants from agronomical important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/technology 9: 957-962
4. Chilton MD, Currier T, Farrand S, Bendich A, Gordon M, Nester E (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci USA 71: 3672-3676
5. Collinge DB, Kragh KM, Mikkelsen JD, Nielsen KK, Rasmussen UK (1993) Plant chitinase. Plant J 3: 31-40
6. Ditta G, Stanfield S, Corbin D, Helinski DR (1980) Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347-7351
7. Fuchs RL, Heeren RA, Gustafson ME, Rogan GJ, Bartnicki DE, Leimgruber RM, Finn RF, Hershman A, Berberich SA (1993) Purification and characterization of microbially expressed neomycin phosphotransferase II (NPTII) protein and its equivalence to the plant expressed protein. Bio/technology 11: 1537-1542
8. Hashizume F, Tsuchiya T, Ugaki M, Niwa Y, Tachibana N, Kowyama Y (1999) Efficient Agrobacterium-mediated transformation and usefulness of a synthetic GFP reporter gene in leading varieties of japonica rice. Plant Biotech 16: 397-401
9. Harms CT, Potrykus I (1978) Fractionation of plant protoplast types by iso-osmotic density gradient centrifugation. Theor Appl Genet 53: 57-63
10. Hayashimoto A, Zhijian L, Murai N (1990) A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants. Plant Physiol 93: 857-863
11. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6: 271-282
12. Jach G, Görnhardt B, Mundy J, Logemann J, Pinsdorf E, Leah R, Schell J, Maas C (1995) Enhanced quantitative resistance against fungal disease by combinatorial expression of different barely antifungal proteins in transgenic tobacco. Plant J 8: 97-109
13. Kim CY, Gal SW, Choe MS, Jeong SY, Lee SY, Cheong YH, Lee SH, Choi YJ, Han C, Kang KY, Cho MJ (1998) A new class II chitinase, Rcht 2, whose induction by fungal elicitor is abolished by protein phosphorus 1 and 2 A inhibitor. Plant Mol Biol 37: 523-534
14. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10: 165-174
15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
16. Lin W, Anuratha CS, Datta K, Potrykus I, Muthukdshnan S, Datta SK (1995) Genetic engineering of rice for resistance to sheath blight. Bio/technology 13: 686-691
17. Mitsuhara I, Ugaki M, Hirochika H, Ohshima M, Murakami T, Gotoh Y, Katayose Y, Nakamura S, Honkura R, Nishimiya S, Ueno K, Mochizuki A, Tanimoto H, Tsugawa H, Otsuki Y, Ohashi Y (1996) Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol 37: 47-59
18. Neale AD, Wahleithner JA, Lund M, Bonnett HT, Kelly A, Meeks-Wagner DR, Peacock WJ, Dennis ES (1990) Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation. Plant Cell 2: 673-84.
19. Nakazaki T, Ikehashi H (1998) Genomic sequence and polymorphism of a rice chitinase gene, Chi4. Breed Sci 48: 371-376
20. Nakazaki T, Tomimoto Y, Ikehashi H, Kowyama Y, Yano M, Yamamoto K, Sasaki T (1997) A novel chitinase in rice (Oryza sativa L.) detected from husk proteins and its gene locus. Breed Sci 47: 363-369
21. Nishizawa Y, Kishimoto N, Saito A, Hibi T (1993) Sequence variation, differential expression and chromosomal location of rice chitinase genes. Mol Gen Genet 241: 1-10
22. Nishizawa Y, Nishio Z, Nakazono K, Soma M, Nakazima E, Ugaki M, Hibi T (1999) Enhanced resistance to blast (Magnaporthe grisea) in transgenic rice by constitutive expression of rice chitinase. Theoret and Appl Genet 99: 383-390
23. Takakura Y, Ito T, Saito H, Inoue T, Komari T, Kuwata S (2000) Flower-predominant expression of a gene encoding a novel class I chitinase in rice (Oryza sativa L.). Plant Mol Biol 42: 883-897
24. Takei N, Nakazaki T, Tsuchiya T, Kowyama Y, Ikehashi H (2000) Isolation and expression of a pistil-specific chitinase gene in rice (Oryza sativa L.). Breed Sci 50: 225-228
25. Zhu Q, Maher EA, Masoud S, Dixon RA, Lamb CJ (1994) Enhanced protection against fungal attack by constitutive coexpression of chitinase and glucanase genes in transgenic tobacco. Bio/technology 12: 807-812