Counterstaining improves visualization of the myenteric plexus in immunolabelled whole-mount preparations

Journal of Fluorescence

Volumn 16, Issue 5, 2006, Pages 655-658

  Mandhan, Parkash ^a   Qi, Bao Quan ^a   Keenan, Jacqueline I ^a   Ismail, Salim ^a   Beasley, Spencer W ^a   Sullivan, Michael J ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

Author keywords

Counterstaining; Immunocytochemistry; Indirect fluorescence; Myenteric plexuses; Whole mount preparations

Indexed keywords

COUNTERSTAINING; IMMUNOCYTOCHEMISTRY; INDIRECT FLUORESCENCE; MYENTERIC PLEXUSES; WHOLE MOUNT PREPARATIONS;

ANTIBODIES; BIOCHEMISTRY; COMPLEXATION; FLUORESCENCE; NEUROLOGY; PROTEINS;

IMMUNOLOGY;

ENOLASE; FLUORESCENT DYE; VASOACTIVE INTESTINAL POLYPEPTIDE;

ANIMAL; ARTICLE; FEMALE; FETUS; FLUORESCENT ANTIBODY TECHNIQUE; HISTOLOGY; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; MYENTERIC PLEXUS; PREGNANCY; RAT; SPRAGUE DAWLEY RAT; STAINING;

ANIMALS; FEMALE; FETUS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; IMMUNOHISTOCHEMISTRY; MYENTERIC PLEXUS; PHOSPHOPYRUVATE HYDRATASE; PREGNANCY; RATS; RATS, SPRAGUE-DAWLEY; STAINING AND LABELING; VASOACTIVE INTESTINAL PEPTIDE;

EID: 33749352580     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10895-006-0120-x     Document Type: Article

References (20)

1. Costa M, Buffa R, Furness JB et al (1980) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65(2):157-165
2. Krammer HJ, Karahan ST, Sigge W et al (1994) Immunohisto-chemistry of markers of the enteric nervous system in whole- mount preparations of the human colon. Eur J Pediatr Surg 4(5):274-278
3. Wester T, O'Briain S, Puri P (1998) Morphometric aspects of the submucous plexus in whole-mount preparations of normal human distal colon. J Pediatr Surg 33(4):619-622
4. Furness JB, Costa M, Franco R et al (1980) Neuronal peptides in the intestine: distribution and possible functions. Adv Biochem Psychopharmacol 22:601-617
5. Costa M, Furness JB, Gibbins IL (1986) Chemical coding of enteric neurons. Prog Brain Res 68:217-239
6. Krammer HJ, Karahan ST, Rumpel E et al (1993) Immunohistochemical visualization of the enteric nervous system using antibodies against protein gene product (PGP) 9.5. Anat Anz 175(4):321-325
7. Fekete E, Resch BA, Benedeczky I (1995) Histochemical and ultra-structural features of the developing enteric nervous system of the human foetal small intestine. Histol Histopathol 10(1):127-134
8. Pickel VM, Teitelman G (1987) Immunocytochemistry of single and multiple antigens with special reference to the enzymes of catecholamine neurons. In: Steinbusch HWM (ed) Monaminergic neurons: light microscopy and ultrastructure. John Wiley & Sons, New York, p 82
9. Schenk EA, Churukian CJ (1974) Immunofluorescence counter-stains. J Histochem Cytochem 22(10):962-966
10. Hrapchak RJ (1980) Immunohistochemistry. In: Sheehan DC, Hrapchak BB (eds) Theory and practice of histotechnology. The CV Mosby Comapny, St. Louis, pp 310-326
11. Pearse BAG (1980) Histochemistry Theoritical and Applied. In: Preparative and optical technology. Churchill Livingston, London, pp 354-358
12. Nichols RL, Me CD (1962) Immunofluorescent studies with trachoma and related antigens. J Immunol 89:545-554
13. Boonpucknavig S, Nairn RC (1967) Serological diagnosis of amoe-biasis by immunofluorescence. J Clin Pathol 20(6):875-878
14. Fry CS, Wilkinson AE (1963) A Note on the Use of Evans Blue as a Background Stain in the Fluorescent Treponemal Antibody Test. Br J Vener Dis 39:190-191
15. Ourth DD (1971) An immunofluorescent test for Toxoplasma gondü in tissues. Immunology 20(4):437-439
16. Brennan LA, Phelps PE, Barber RP (1986) An improved method of toluidine blue counterstaining of neurons in immunocytochemical preparations. Stain Technol 61(5):279-285
17. Jones KH, Kniss DA (1987) Propidium iodide as a nuclear counterstain for immunofluorescence studies on cells in culture. J Histochem Cytochem 35(1):123-125
18. Closs O, Aarli JA (1973) Evans blue as counterstain in the demonstration of muscle antibodies by immunofluorescence in myasthenia gravis. J Clin Path 27:162-167
19. El-Badawi A, Schenk EA (1967) Histochemical methods for separate, consecutive and simultaneous demonstration of acetylcholinesterase and norepinephrine in cryostat sections. J Histochem Cytochem 15(10):580-588
20. Fey H (1972) Eriochrome black, a means for reduction of nonspecificity in immunofluorescence. Pathol Microbiol 38(4):271-277