Reversible and irreversible effects of chemical fixation on the NMR properties of single cells

Magnetic Resonance in Medicine

Volumn 56, Issue 4, 2006, Pages 927-931

  Purea, Armin ^a   Webb, Andrew G ^b  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

Chemical fixation; Diffusion coefficient; Magnetic resonance histology; Membrane permeability; Relaxation times

Indexed keywords

CYTOLOGY; DIFFUSION; RELAXATION TIME; SURFACE DIFFUSION;

APPARENT DIFFUSION COEFFICIENT; CHEMICAL FIXATION; IRREVERSIBLE EFFECTS; MEMBRANE PERMEABILITY; RELAXATION TIMES; STATISTICALLY SIGNIFICANT DIFFERENCE; SUBSTANTIAL REDUCTION; XENOPUS LAEVIS OOCYTES;

NUCLEAR MAGNETIC RESONANCE;

BUFFER; CONTRAST MEDIUM; GADOLINIUM; PERFLUOROTRIBUTYLAMINE; WATER;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; CONTROLLED STUDY; CYTOPLASM; DIFFUSION COEFFICIENT; HISTOLOGY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; OOCYTE; RELAXATION TIME; XENOPUS LAEVIS;

EID: 33749173142     PISSN: 07403194     EISSN: 15222594     Source Type: Journal    
DOI: 10.1002/mrm.21018     Document Type: Article

References (20)

1. Johnson GA, Cofer GP, Fubara B, Gewalt SL, Hedlund LW, Maronpot RR. Magnetic resonance histology for morphologic phenotyping. J Magn Reson Imaging 2002;16:423-429.
2. Matsuda Y, Utsuzawa S, Kurimoto T, Haishi T, Yamazaki Y, Kose K, Anno I, Marutani M. Super-parallel MR microscope. Magn Reson Med 2003;50:183-189.
3. Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotech Histochem 1994;69:177-179.
4. Helander KG, Widehn S, Helander HF. Kinetic studies of glutaraldehyde binding in liver. Biotech Histochem 2002;77:207-212.
5. Yong-Hing CJ, Obenaus A, Stryker R, Tong K, Sarty GE. Magnetic resonance imaging and mathematical modeling of progressive formalin fixation of the human brain. Magn Reson Med 2005;54:324-332.
6. Bossart EL, Inglis BA, Silver XS, Mareci TH. The effect of fixative solutions in magnetic resonance imaging. In: Proceedings of the 7th Annual Meeting of ISMRM, Philadelphia, PA, USA, 1999. p 1928.
7. Thickman DI, Kundel HL, Wolf G. Nuclear magnetic resonance characteristics of fresh and fixed tissue: the effect of elapsed time. Radiology 1983;148:183-185.
8. Tovi M, Ericsson A. Measurements of T1 and T2 over time in formalin-fixed human whole-brain specimens. Acta Radiol 1992;33:400-404.
9. Pattany PM, Puckett WR, Klose KJ, Quencer RM, Bunge RP, Kasuboski L, Weaver RG. High-resolution diffusion-weighted MR of fresh and fixed cat spinal cords: evaluation of diffusion coefficients and anisotropy. AJNR Am J Neuroradiol 1997;18:1049-1056.
10. Bainbridge A, Schmierer K, Miller DH, Ordidge RJ, Yousry TA. Changes in T1 and T2 in formalin fixed tissue compared to fresh tissue at 7 tesla. In: Proceedings of the 12th Annual Meeting of ISMRM, Kyoto, Japan, 2004. p 2316.
11. Thelwall PE, Grant SC, Stanisz GJ, Blackband SJ. Human erythrocyte ghosts: exploring the origins of multiexponential water diffusion in a model biological tissue with magnetic resonance. Magn Reson Med 2002;48:649-657.
12. Shepherd TM, Thelwall PE, Stanisz GJ, Blackband SJ. Paraformaldehyde and Karnovsky's fixative alter microstructure in an erythrocyte ghost tissue model. In: Proceedings of the 12th Annual Meeting of ISMRM, Kyoto, Japan, 2004. p 580.
13. Li JG, Stanisz GJ, Henkelman RM. Integrated analysis of diffusion and relaxation of water in blood. Magn Reson Med 1998;40:79-88.
14. Sehy JV, Ackerman JJH, Neil JJ. Evidence that both fast and slow water ADC components arise from intracellular space. Magn Reson Med 2002;48:765-770.
15. Sehy JV, Banks AA, Ackerman JJH, Neil JJ. Importance of intracellular water apparent diffusion to the measurement of membrane permeability. Biophys J 2002;83:2856-2863.
16. Sehy JV, Ackerman JJH, Neil JJ. Apparent diffusion of water, ions, and small molecules in the Xenopus oocyte is consistent with Brownian displacement. Magn Reson Med 2002;48:42-51.
17. Sehy JV, Zhao L, Xu JQ, Rayala HJ, Ackerman JJH, Neil JJ. Effects of physiologic challenge on the ADC of intracellular water in the Xenopus oocyte. Magn Reson Med 2004;52:239-247.
18. Sehy JV, Ackerman JJH, Neil JJ. Water and lipid MRI of the Xenopus oocyte. Magn Reson Med 2001;46:900-906.
19. Hsu EW, Aiken NR, Blackband SJ. Nuclear magnetic resonance microscopy of single neurons under hypotonic perturbation. Am J Physiol 1996;271:C1895-C1900.
20. Winkelman JGM, Beenackers AACM. Correlations for the density and viscosity of aqueous formaldehyde solutions. Indust Eng Chem Res 2000;39:557-562.