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Volumn 128, Issue 38, 2006, Pages 12420-12421

A cost-effective method for the optical transduction of chemical reactions. Application to hyaluronidase inhibitor screening with polyarginine-counteranion complexes in lipid bilayers

Author keywords

[No Author keywords available]

Indexed keywords

ANION; ENZYME INHIBITOR; HYALURONIDASE; POLYARGININE;

EID: 33749164306     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja064732o     Document Type: Article
Times cited : (37)

References (24)
  • 19
    • 12344275753 scopus 로고    scopus 로고
    • 1f support for a carrier mechanism includes biphasic kinetics for oligoarginine removal with heparin from and across intact vesicles, and U-tube activity: Sakai, N.; Takeuchi, T.; Futaki, S.; Matile, S. ChemBioChem 2005, 6, 114-122.
    • (2005) ChemBioChem , vol.6 , pp. 114-122
    • Sakai, N.1    Takeuchi, T.2    Futaki, S.3    Matile, S.4
  • 20
    • 0344012169 scopus 로고    scopus 로고
    • (c) Sensitivity decreases with oligoarginine length: Sakai, N.; Matile S. J. Am. Chem. Soc. 2003, 125, 14348-14356.
    • (2003) J. Am. Chem. Soc. , vol.125 , pp. 14348-14356
    • Sakai, N.1    Matile, S.2
  • 21
    • 33749175680 scopus 로고    scopus 로고
    • note
    • ATP = 4 with pR-DP transducers. This difference was sufficient to detect kinase activity but incompatible with binary "on/off" ATP/ADP discrimination. Studies on the limitations of the universal adaptability of optical counterion-polyion transducers are ongoing with particular emphasis on covalent in situ amplification of poorly recognized inactivating as well as activating analytes.
  • 24
    • 33749160241 scopus 로고    scopus 로고
    • note
    • The detectability of substrate immobilization on solid support with pR-DP transducers identified another attractive field of application of the here reported method (see Supporting Information). Substrate immobilization is also convenient for Michaelis-Menten analysis to determine the nature of inhibition.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.