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note
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ATP = 4 with pR-DP transducers. This difference was sufficient to detect kinase activity but incompatible with binary "on/off" ATP/ADP discrimination. Studies on the limitations of the universal adaptability of optical counterion-polyion transducers are ongoing with particular emphasis on covalent in situ amplification of poorly recognized inactivating as well as activating analytes.
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note
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The detectability of substrate immobilization on solid support with pR-DP transducers identified another attractive field of application of the here reported method (see Supporting Information). Substrate immobilization is also convenient for Michaelis-Menten analysis to determine the nature of inhibition.
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