An optimized method for automated analysis of algal pigments by HPLC

Marine Chemistry

Volumn 102, Issue 3-4, 2006, Pages 267-275

  van Leeuwe, M A ^a   Villerius, L A ^b   Roggeveld, J ^a,c   Visser, R J W ^a   Stefels, J ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b National Institute for Coastal and Marine Management RIKZ   (Netherlands)

^c UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

Automation; Freeze drying; HPLC pigment analyses; Microalgae; Water packing

Indexed keywords

ACETONE; AUTOMATION; FREEZE DRYING; LIQUID CHROMATOGRAPHY; METHANOL; PHYTOPLANKTON; PIGMENT; SAMPLING;

ALGAE; BACILLARIOPHYCEAE; EMILIANIA HUXLEYI; HAPTOPHYCEAE; PHAEOCYSTIS ANTARCTICA; PHAEOCYSTIS GLOBOSA; PRASINOPHYCEAE; PYRAMIMONAS SP.; THALASSIOSIRA;

EID: 33749080729     PISSN: 03044203     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.marchem.2006.05.003     Document Type: Article

References (28)

1. Admiraal W., and Werner D. Utilization of limiting concentrations of ortho-phosphate and production of extracellular organic phosphates in cultures of marine diatoms. J. Plankton Res. 5 (1983) 495-513
2. Barrett J., and Jeffrey S.W. Chlorophyllase and formation of an atypical chlorophyllide in marine algae. Plant Physiol. 39 (1964) 44-47
3. Bowles N.D., Paerl H.W., and Tucker J. Effective solvents and extraction periods employed in phytoplankton carotenoid and chlorophyll determinations. Can. J. Fish. Aquat. Sci. 42 (1985) 1127-1131
4. Brereton R.G., Rahmani A., Liang Y.-Z., and Kvalheim O.M. Investigation of the allomerisation reaction of chlorophyll a: use of diode array HPLC, mass spectrometry and chemometric factor analysis for the detection of early products. Photochem. Photobiol. 59 (1994) 99-110
5. Buffan-Dubau E., and Carman K.R. Extraction of benthic microalgal pigments for HPLC analyses. Mar. Ecol., Prog. Ser. 204 (2000) 293-297
6. Chen N., Bianchi T.S., and Bland J.M. Novel decomposition products of chlorophyll-a in continental shelf (Louisiana shelf) sediments: formation and transformation of carotenol chlorine esters. Geochim. Cosmochim. Acta 67 11 (2003) 2027-2042
7. Claustre H., Hooker S.B., van Heukelem L., Berthon J.-F., Barlow R., Ras J., Sessions H., Targa C., Thomas C.S., van der Linde D., and Marty J.-C. An intercomparison of HPLC phytoplankton pigment methods using is situ samples: application to remote sensing and database activities. Mar. Chem. 85 (2004) 41-61
8. Draziewicz M. Chlorophyllase: occurrence, functions, mechanisms of action, effects of external and internal factors. Photosynthesis 30 (1994) 321-331
9. Jeffrey S.W. Profiles of photosynthetic pigments in the ocean using thin-layer chromatography. Mar. Biol. 26 (1974) 101-110
10. Jeffrey S.W., and Hallegraeff G.M. Chlorophyllase distribution in ten classes of phytoplankton: a problem for chlorophyll analysis. Mar. Ecol., Prog. Ser. 35 (1987) 293-304
11. Jeffrey S.W., Mantoura R.F.C., and Wright S.W. Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods (1997), UNESCO Publishing, Paris 661 pp.
12. Geider R.J., LaRoche J., Greene R.M., and Olaizola M. Response of the photosynthetic apparatus of Phaeodactylum tricornutum (Bacillariophyceae) to nitrate, phosphate, or iron starvation. J. Phycol 29 (1993) 755-766
13. Gibb S.W., Cumming D.G., Irigoien X., Barlow R.G., Fauzi R., and Mantoura C. Phytoplankton pigment chemotaxonomy of the northeastern Atlantic. Deep-Sea Res. II 48 (2001) 295-823
14. Kowalewska G., and Szymczak M. Influence of selected abiotic factors on the decomposition of chlorophylls. Oceanologia 43 3 (2001) 315-328
15. 3 of marine phytoplankton by reverse-phase-C18-high-performance liquid chromatography. J. Phycol. 28 (1992) 708-712
16. Lasata M., Bidigare R.R., Ondrusek M.E., and Kennicutt II M.C. HPLC analysis of algal pigments: a comparison exercise among laboratories and recommendations for improved analytical performance. Mar. Chem. 51 (1996) 315-324
17. Mantoura R.F.C., Wright S.W., Jeffrey S.W., Barlow R.G., and Cummings D.E. Filtration and storage of pigments from microalgae. In: Jeffrey S.W., Mantoura R.F.C., and Wright S.W. (Eds). Phytoplankton Pigments in Oceanography: Guidelines to Modern methods (1997), UNESCO, Paris 283-305
18. Nakamura A., and Watanabe T. Separation and determination of minor photosynthetic pigments by reversed-phase HPLC with minimal alteration of chlorophylls. Anal. Sci. 17 (2001) 503-508
19. Nelson J.R. Rates and possible mechanism of light-dependent degradation of pigments in detritus derived from phytoplankton. J. Mar. Res. 51 (1993) 155-179
20. Stock, R., Rice, C.B.F., 1967. Chromatographic methods. Science Paperbacks, Chapman and Hall Ltd. Northumberland Press Limited. 256 pp.
21. van Leeuwe M.A., and Stefels J. Effects of iron and light stress on the biochemical composition of Antarctic Phaeocystis sp. (Prymnesiophyceae). II. Pigment composition. J. Phycol. 34 (1998) 496-503
22. van Leeuwe M.A., de Baar H.J.W., and Veldhuis M.J.W. Pigment distribution in the Pacific region of the Southern Ocean (autumn 1995). Polar Biol. 19 (1998) 348-353
23. Wright S.W., and Jeffrey S.W. Fucoxanthin pigment markers of marine phytoplankton analysed by HPLC and HPLTC. Mar. Ecol., Prog. Ser. 38 (1987) 259-266
24. Wright S.W., Jeffrey S.W., Mantoura R.F.C., Llewellyn C.A., Bjornland T., Repeta D., and Welschmeyer N. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Mar. Ecol., Prog. Ser. 77 (1991) 183-196
25. Wright S.W., Thomas D.P., Marchant H.J., Higgins H.W., Mackey M.D., and Mackey D.J. Analysis of phytoplankton of the Australian sector of the Southern Ocean: comparisons of microscopy and size-frequency data with interpretations of pigment HPLC data using the CHEMTAX matrix factorisation program. Mar. Ecol., Prog. Ser. 144 (1996) 0285-0298
26. Wright S.W., Jeffrey S.W., and Mantoura R.F.C. Evaluation of methods and solvents for pigment extraction. In: Jeffrey S.W., Mantoura R.F.C., and Wright S.W. (Eds). Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods (1997), UNESCO, Paris 261-282
27. Zapata M., and Garrido J.L. Influence of injection conditions in reverse-phase-high-performance liquid chromatography of chlorophylls and carotenoids. Chromatographia 31 11/12 (1991) 589-594
28. Zapata M., Jeffrey S.W., Wright S.W., Rodriguez F., Garrido J.L., and Clementson L. Photosynthetic pigments in 37 species (65 strains) of Haptophyta: implications for oceanography and chemotaxonomy. Mar. Ecol., Prog. Ser. 270 (2004) 83-102