Detection of human immunodeficiency virus-1 RNA and DNA by extractive and in situ PCR in unprocessed semen and seminal fractions isolated by semen-washing procedure

Human Reproduction

Volumn 21, Issue 6, 2006, Pages 1525-1530

  Persico, Tiziana ^a   Savasi, Valeria ^a   Ferrazzi, Enrico ^a   Oneta, Monica ^a   Semprini, A E ^a   Simoni, Giuseppe ^b  

^a UNIVERSITY OF MILAN   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

HIV 1; IS PCR; Nested PCR; Semen wash; Serodiscordant

Indexed keywords

EFAVIRENZ; INDINAVIR; LAMIVUDINE; NELFINAVIR; NEVIRAPINE; RITONAVIR; STAVUDINE; VIRUS DNA; VIRUS RNA; ZALCITABINE; ZIDOVUDINE;

ADULT; ARTICLE; CLINICAL PROTOCOL; CONTROLLED STUDY; DNA DETERMINATION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MAJOR CLINICAL STUDY; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; RNA ANALYSIS; SEMEN ANALYSIS; SEMINAL PLASMA; SPERM DONOR; SPERMATOZOON; VIREMIA; VIRUS LOAD;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 33749005925     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/del004     Document Type: Article

References (40)

1. Anderson DJ et al. (1990) Presence of HIV-1 in semen. In Alexander NJ, Gabelnick HL and Spieler JM (eds) Heterosexual Transmission of AIDS. Proceedings of the Second Contraceptive Research and Development (CONRAD) Program International Workshop, Held in Norfolk, Virginia, February 1-3, 1989. Wiley-Liss, New York, pp. 167-180.
2. Baccetti B, Benedetto A, Burrini AG, Collodel G, Elia C, Piomboni P, Renieri T, Sensini C and Zaccarelli M (1991) HIV-1 particles detected in spermatozoa of patients with AIDS. J Submicrosc Cytol Pathol 23,339-345
3. Baccetti B, Benedetto A, Burrini AG, Collodel G, Ceccarini EC, Crisa N, Di Coro A, Estenoz M, Garbuglia AR, Massacesi A et al. (1994) HIV-1 particles in spermatozoa of patients with AIDS and their transfer into the oocytes. J Cell Biol 127,903-914.
4. Baccetti B, Benedetto A, Burrini AG, Collodel G, Elia C, Piomboni P, Renieri T, Sensini C and Zaccarelli M (1991) HIV particles detected in spermatozoa of patients with AIDS. J Submicrosc Cytol Pathol 23,339-345.
5. Baccetti B, Benedetto A, Collodel G, Di Caro A, Garbuglia AR and Piomboni P (1998) The debate on the presence of HIV-1 in human gametes. J Reprod Immunol 41,41-67.
6. Bagasra O and Pomerantz RJ (1993) HIV-1 provirus is demonstrated in PB monocytes in vivo: A study utilizing an in situ PCR. AIDS Res Hum Retroviruses 9,69-76.
7. Bagasra O et al. (1990) Presence of HIV-1 in sperm of patients with HIV-1/AIDS. Mol Androl 2,109-125.
8. Bagasra O, Farzadegan H, Seshamma T, Oakes JW, Saah A and Pomerantz RJ (1994) Detection of HIV-1 proviral DNA in sperm from HIV-1 infected men. AIDS 8,1669-1674.
9. Bagasra O, Freund M, Weidmann J and Harley G (1988) Interaction of human immunodeficiency virus with human sperm in vitro. J Acquir Immune Defic Syndr 1,431-435.
10. Bagasra O, Hauptman SP, Lischner HW, Sachs M and Pomerantz RJ (1992) Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction. N Engl J Med 326,1385-1391.
11. Borzy MS, Connell RS and Kiessling AA (1988) Detection of human immunodeficiency virus in cell-free seminal fluid. J Acquir Immune Defic Syndr 1,419-424.
12. Bujan L, Daudin M, Alvarez M, Massip P, Puel J and Pasquier C (2002) Intermittent human immunodeficiency type 1 virus (HIV-1) shedding in semen and efficiency of sperm processing despite high seminal HIV-1 RNA levels. Fertil Steril 78,1321-1323.
13. Bujan L, Daudin M, Matusuda T, Righi L, Thauvin L, Berges L, Izopet J, Berrerebi A, Massip P and Pasquier C (2004) Factors of intermittent HIV-1 excretion in semen and efficiency of sperm processing in obtaining spermatozoa without HIV-1 genomes. AIDS 18,757-766.
14. Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162,156-159.
15. Coll O, Vidal R, de Tejada BM, Ballesca JL, Azulay M and Vanrell JA (1999) Management of HIV-1 serodiscordant couples. The clinician point of view. Contracept Fertil Sex 27,399-404.
16. Dussaix E, Guetard D, Dauget C, D'Almeida M, Auer J, Ellrodt A, Montagnier L and Auroux M (1993) Spermatozoa as potential carriers of HIV-1. Res Virol 144,487-495.
17. Garrido N, Meseguer M, Bellever J, Remohi J, Simon C and Pellicer A (2004) Report of the results of 2 year programme of sperm wash and ICSI treatment for human immunodeficiency virus and hepatitis C virus serodiscordant couples. Hum Reprod 19,2581-2586.
18. Garrido N, Zuzuarregui JL, Meseguer M, Simon C, Remohi J and Pellicer A (2002) Sperm and oocyte donor selection and management: Experience of 10 year follow-up of more than 2100 candidates. Hum Reprod 17,3142-3148
19. Gilling-Smith C (2000) HIV-1 prevention. Assisted reproduction in HIV-1-discordant couples. AIDS Read 10,581-587.
20. Gobert B, Amiel C, Tang JQ, Barbarino P, Bene MC and Faure G (1990) CD4-like molecules in human sperm. FEBS Lett 261,339-342.
21. Hamed KA, Winters MA, Holodniy M, Katzennstein DA and Merigan TC (1993) Detection of human immunodeficiency virus type 1 in semen: Effects of disease stage and nucleoside therapy. J Infect Dis 167,798-802.
22. Hanabusa H, Kujii N, Kato S, Tagami H, Kanedo S, Tanaka H and Yoshimura Y (2000) An evaluation of semen processing methods for eliminating HIV-1. AIDS 14,1611-1616.
23. Kim LU, Jonhson MR, Barton S, Nelson MR, Sontag G, Smith JR, Gotch FM and Gilmour JW (1999) Evaluation of sperm washing as a potential method of reducing HIV-1 transmission in HIV-1-discordant couples wishing to have children. AIDS 13,645-651.
24. Leruez-Ville M, De Almeida M, Tachet A, Dulioust E, Guibert J, Mandelbrot L, Salmon D, Jouannet P and Rouzioux C (2002) Assisted reproduction in HIV-1 serodifferent couples: The need for viral validation of processed semen. AIDS 16,2267-2273.
25. Marina S, Marina F, Alcolea R, Exposito R, Huguet J, Nadal J and Verges A (1998) Human immunodeficiency virus type 1-serodiscordant couples can bear healthy children after undergoing intrauterine insemination. Fertil Steril 70,35-39.
26. Mermin JH, Holodniy M, Katzenstein DA and Merigan TC (1991) Detection of human immunodeficiency virus DNA and RNA in semen by the polymerase chain reaction. J Infect Dis 164,769-772.
27. Meseguer M, Garrido N, Gimeno C, Remohi J, Simon C and Pellicer A (2002) Comparison of polymerase chain reaction-dependent methods for determining the presence of human immunodeficiency virus and hepatitis C virus in washed sperm. Fertil Steril 78,1199-1202.
28. Miller VE and Scofield VL (1990) Transfer of HIV-1 by semen: Role of sperm. In Alexander NJ, Gabelnick HL and Spieler JM (eds) Heterosexual Transmission of AIDS. Proceedings of the Second Contraceptive Research and Development (CONRAD) Program International Workshop, Held in Norfolk, Virginia, February 1-3, 1989. Wiley-Liss, New York, pp. 147-154.
29. Muciaccia B, Uccini S, Filippini A, Ziparo E, Paraire F, Baroni CD and Stefanini M (1998) Presence and cellular distribution of HIV-1 in the testes of seropositive subjects: An evaluation by in situ PCR hybrydization. FASEB J 12,151-163.
30. Nuovo GJ, Becker J, Simsir A, Margiotta M, Khalife G and Shevchuk M (1994) HIV-1 nucleic acids localize to the spermatogonia and their progeny. A study by polymerase chain reaction in situ hybridization. Am J Pathol 144,1142-1148.
31. Ohl J, Partisani M, Wittemer C, Schmitt MP, Cranz C, Stoll-Keller F, Rongieres C, Bettahar-Lebugle K, Lang JM and Nisand I (2003) Assisted reproduction techniques for HIV serodiscordant couples: 18 months of experience. Hum Reprod 18,1244-1249.
32. Pasquier C, Daudin M, Righi L, Berges L, Thauvin L, Berrebi A, Massip P, Puel J, Bujan L and Izopet J (2000) Sperm washing and virus nucleic acid detection to reduce HIV-1 and hepatitis C virus transmission in serodiscordant couples wishing to have children. AIDS 14,2093-2099.
33. Politch JA, Xu C, Tucker L, Anderson DJ (2004). Separation of human immunodeficiency virus type 1 from motile sperm by the double tube gradient method versus other methods. Fertil Steril 81,440-447.
34. Pudney J (1990) Caveats associated with identifying HIV-1 using transmission electron microscopy. In Alexander NJ, Gabelnick HL and Spieler JM (eds) Heterosexual Transmission of AIDS. Proceedings of the Second Contraceptive Research and Development (CONRAD) Program International Workshop, Held in Norfolk, Virginia, February 1-3, 1989. Wiley-Liss, New York, pp. 197-204.
35. Quayle AJ, Xu C, Mayer KH and Anderson DJ (1997) T-lymphocytes and macrophages, but not motile spermatozoa, are a significant source of human immunodeficiency virus in semen. J Infect Dis 176,960-968.
36. Scofield VL, Rao B, Broder S, Kennedy C, Wallace M, Graham B and Poiesz BJ (1994) HIV interaction with sperm. AIDS 8,1733-1736.
37. Semprini AE, Levi-Setti P, Bozzo M, Ravizza M, Taglioretti A, Sulpizio P, Albani E, Oneta M and Pardi G (1992). Insemination of HIV-1-negative women with processed semen of HIV-1-positive partners. Lancet 340,1317-1319.
38. Shevchuk MM, Nuovo GJ and Khalife G (1998) HIV-1 in testis: Quantitative histology and HIV-1 localization in germ cells. J Reprod Immunol 41,69-79.
39. Van Voorhis BJ, Martinez A, Mayer K and Anderson DJ (1991) Detection of HIV-1 in semen from seropositive men using culture and polymerase chain detection deoxyribonucleic acid amplification techniques. Fertil Steril 55,588-594.
40. Xu C, Politch JA, Mayer KH and Anderson DJ (2005) Human immunodeficiency virus type-1 episomal cDNA in semen. AIDS Res Ther 2,9.