Thermal stability of recombinant green fluorescent protein (GFPuv) at various pH values

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 114, Issue 1-3, 2004, Pages 469-483

  Penna, Thereza Christina Vessoni ^a   Ishii, Marina ^a   Junior, Adalberto Pessoa ^a   Cholewa, Olivia ^b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b MOLECULAR PROBES INC   (United States)

Author keywords

Acetate; Decimal reduction time; Fluorescence intensity; Green fluorescent protein; Phosphate; Thermal stability; Three phase partitioning

Indexed keywords

CELLS; CHROMATOGRAPHY; ESCHERICHIA COLI; FLUORESCENCE; PH EFFECTS; THERMODYNAMIC STABILITY;

FLUORESCENT BIOLOGICAL INDICATORS; GREEN FLUORESCENT PROTEINS (GFP); HYDROPHOBIC INTERACTION CHROMATOGRAPHY; MOIST-HEAT TREATMENT;

PROTEINS;

ACETIC ACID; GREEN FLUORESCENT PROTEIN; PHOSPHATE; RECOMBINANT PROTEIN; TROMETAMOL; ACETIC ACID DERIVATIVE; BUFFER; PHOTOPROTEIN;

ANIMAL CELL; CHROMATOGRAPHY; CONFERENCE PAPER; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PH; PROTEIN DENATURATION; PROTEIN ISOLATION; THERMOSTABILITY; ARTICLE; BIOTECHNOLOGY; CHEMISTRY; HEAT; KINETICS; METABOLISM; METHODOLOGY; SPECTROFLUOROMETRY; TEMPERATURE; TIME;

ANIMALIA; ESCHERICHIA COLI;

ACETATES; BIOTECHNOLOGY; BUFFERS; CHROMATOGRAPHY; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HEAT; HYDROGEN-ION CONCENTRATION; KINETICS; LUMINESCENT PROTEINS; PHOSPHATES; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; TEMPERATURE; TIME FACTORS;

EID: 33748928777     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1385/ABAB:114:1-3:469     Document Type: Conference Paper

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