Use of hydrocyclones for mammalian cell retention: Separation efficiency and cell viability (Part 1)

Engineering in Life Sciences

Volumn 6, Issue 4, 2006, Pages 347-354

  Elsayed, E A ^a   Medronho, R A ^b   Wagner, R ^c   Deckwer, W D ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^c MILTENYI BIOTEC GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FLOW INTERACTIONS; PRESSURE DROP; SEPARATION; VORTEX FLOW;

CELL VIABILITY; FLOW RATIO; FLOW SPLIT; HYDROCYCLONES; MAMMALIAN CELL RETENTION;

ANIMAL CELL CULTURE;

ANIMAL CELL CULTURE; FLOW INTERACTIONS; PRESSURE DROP; SEPARATION; VORTEX FLOW;

CELLS AND CELL COMPONENTS; PRESSURE DROP; SEPARATION;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL SEPARATION; CELL STRAIN BHK; CELL SUSPENSION; CELL VIABILITY; CENTRIFUGE; CHO CELL; CONTROLLED STUDY; DEVICE; FLOW RATE; HELA CELL; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; PRESSURE;

MAMMALIA;

EID: 33748928281     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.200620137     Document Type: Article

References (37)

1. L. R. Castilho, R. A. Medronho, Cell retention devices for suspended-cell perfusion cultures, in Tools and Applications of Biochemical Engineering Science (Eds: K. Schügerl, A.-P. Zeng), Vol. 74 (Advances in Biochemical Engineering and Biotechnology), Springer Verlag, Berlin 2002, 129-169.
2. A.-P. Zeng, W.-D. Deckwer, Model simulation and analysis of perfusion culture of mammalian cells at high cell density, Biotechnol. Prog. 1999, 15, 373-382.
3. S. M. Woodside, B. D. Bowen, J. M. Piret, Mammalian cell retention devices for stirred perfusion bioreactors, Cytotechnology 1998, 28, 163-175.
4. V.M. Gorenflo, L. Smith, B. Dedinsky, B. Persson, J. M. Piret, Scale up and optimization of an acoustic filter for 200 L/day perfusion of a CHO cell culture, Biotechnol. Bioeng. 2002, 80, 438-444.
5. P. Himmelfarb, P. S. Thayer, H. E. Martin, Spin filter culture: The propagation of mammalian cells in suspension, Science 1969, 164, 555-557.
6. Y.M. Deo, M.D. Mahadevan, R. Fuchs, Practical considerations in operation and scale-up of spin filter in based bioreactors for monoclonal antibody production, Biotechnol. Prog. 1996, 12, 57-64.
7. R. van Reis, L. C. Leonard, C. C. Hsu, S. E. Builder, Industrial scale harvest of protein from mammalian cell culture by tangential flow filtration, Biotechnol. Bioeng. 1991, 38, 413-422.
8. S. Mercille, M. Johnson, R. Lemieux, B. Massie, Filtration-based perfusion of hybridoma cultures in protein-free medium: Reduction of membrane fouling by medium supplementation with DNase I, Biotechnol. Bioeng. 1994, 43, 833-846.
9. L. R. Castilho, F. B. Anspach, W. D. Deckwer, An integrated process for mammalian cell perfusion cultivation and product purification using a dynamic filter, Biotechnol. Prog. 2002, 18, 776-781.
10. L. R. Castilho, F. B. Anspach, CFD-aided design of a dynamic filter for mammalian cell separation, Biotechnol. Bioeng. 2003, 83, 514-524.
11. B. C. Batt, R. H. Davis, D. S. Kompala, Inclined sedimentation for selective retention of viable hybridomas in a continuous suspension bioreactor, Biotechnol. Prog. 1990, 6, 458-464.
12. M. Tokashiki, H. Takamatsu, Perfusion culture apparatus for suspended mammalian cells, Cytotechnology 1993, 13, 149-159.
13. M. Johnson, S. Lanthier, B. Massie, G. Lefebvre, A. A. Kamen, Use of the Centritech Lab centrifuge for perfusion culture of hybridoma cells in protein-free medium, Biotechnol. Prog. 1999, 12, 855-864.
14. M. Takagi, M. Ilias, T. Yoshida, Selective retention of active cells employing low centrifugal force at the medium change during suspension culture of Chinese hamster ovary cells producing tPA, J. Biosci. Bioeng. 2000, 89, 340-344.
15. V. M. Gorenflo, S. Angepat, B. D. Bowen, J. M. Piret, Optimization of an acoustic cell filter with a novel Air-Backflush System, Biotechnol. Prog. 2003, 19, 30-36.
16. D. Voisard, F. Meuwly, P. A. Ruffieux, G. Baer, A. Kadouri, Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells, Biotechnol. Bioeng. 2003, 82, 751-765.
17. I.Z. Shirgaonkar, S. Lanthier, A. Kamen, Acoustic cell filter: a proven cell retention technology for perfusion of animal cell cultures, Biotechnol. Adv. 2004, 22, 433-444.
18. W. S. P. Pui, F. Trampler, S. A. Sonderhoff, M. Groeschl, D. G. Kilburn, J. M. Piret, Batch and semicontinuous aggregation and sedimentation of hybridoma cells by acoustic resonance fields, Biotechnol. Prog. 1995, 11, 146-152.
19. H. Yuan, D. Rickwood, I. C. Smyth, M. T. Thew, An investigation into the possible use of hydrocyclones for the removal of yeast from beer, Bioseparation 1996, 6, 159-163.
20. J.J. Cilliers, S. T. L. Harrison, The application of mini hydrocyclones in the concentration of yeast suspension, Chem. Eng. J. 1997, 65, 21-26.
21. V.M. Matta, R. A. Medronho, A new method for yeast recovery in batch ethanol fermentation: Filter aid filtration followed by separation of yeast from filter oid using hydrocyclones, Bioseparation 2000, 9, 43-53.
22. M. Lübberstedt, F. B. Anspach, W. D. Deckwer, R. A. Medronho, Abtrennung tierischer Zellen mit Hydrozyklonen, Chem. Eng. Tech. 2000, 72, 1089-1090.
23. A. Jockwer, R. A. Medronho, R. Wagner, F. B. Anspach, W. D. Deckwer, The use of hydrocyclones for mammalian cell retention in perfusion bioreactors, in Animal Cell Technology: From Target to Market (Eds: E. Linder-Olsson, N. Chatzissavidou, E. Lüllau), Kluwer Academic Publishers, Dordrecht 2001, 301-305.
24. R. A. Medronho, J. Schuetze, W. D. Deckwer. Numerical simulation of hydrocyclones for cell separation, Lat. Am. Appl. Res. 2005, 35, 1-8.
25. H. S. Conradt, M. Ausmeier, K. E. J. Dittmar, H. Hauser, W. Lindenmaier, Secretion of glycosylated human interleukin-2 by recombinant mammalian cell lines, Carbohydr. Res. 1986, 149, 443-450.
26. R. Wagner, J. Lehmann. The growth and productivity of recombinant animal cells in a bubble-free aeration system, Trends Biotechnol. 1988, 6, 101-104.
27. W. D. Deckwer, R. A. Medronho, B. Anspach, M. Lübberstedt, Method for separating viable cells from cell suspensions, US 6,878,545 B2, EP 1 2 80 885 B1, Proprietor: GBF, 2005.
28. C. Legrand, J. M. Bour, C. Jacob, J. Capiaumont, A. Martial, A. Marc et al., Lactate dehydrogenase (LDH) activity of the number of dead cells in the medium of cultured eukaryotic cells as marker, J. Biotechnol. 1992, 25, 231-243.
29. I. Vermes, C. Haanen, H. Steffens-Nakken, C. P. Reutelingsperger, A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V, J. Immunol. Meth. 1995, 184, 39-51.
30. J. F. Petersen, L. V. McIntire, E. T. Papoutsakis, Shear sensitivity of cultured hybridoma cells (CRL-8018) depends on mode of growth, culture age and metabolite concentration, J. Biotechnol. 1988, 7, 229-246.
31. J. B. Joshi, C. B. Elias, M. S. Patole, Role of hydrodynamic shear in the cultivation of animal, plant and microbial cells, Chem. Eng. J. 1996, 62, 121-141.
32. M. Al-Rubeai, R. P. Singh, M. H. Goldmann, A. N. Emery, Death mechanisms of animal cells in conditions of intensive agitation, Biotechnol. Bioeng. 1995, 45, 463-473.
33. M. Al-Rubeai, R.P. Singh, Apoptosis in cell culture, Curr. Opin. Biotechnol. 1998, 9, 152-156.
34. R. P. Singh, M. Al-Rubeai, Apoptosis, in Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (Eds: M. C. Flickinger, S. W. Drew), John Wiley & Sons, Chichester, UK, 1999, 191-201.
35. S. Dimmeler, J. Haendeler, V. Rippmann, M. Nehls, A. M. Zeiher, Shear stress inhibits apoptosis of human endothelial cells, FEBS Lett. 1996, 399, 71-74.
36. C. Born, Z. Zhang, M. Al-Rubeai, C. R. Thomas, Estimation of disruption of animal cells by laminar shear stress, Biotechnol. Bioeng. 1992, 40, 1004-1010.
37. C. G. Nevaril, E. C. Lynch, C. P. Alfrey, J. D. Hellums, Erythrocyte damage and destruction induced by shearing stress, J. Lab. Clin. Med. 1968, 71, 785-790.