Simple method for RNA preparation from cyanobacteria

Journal of Phycology

Volumn 42, Issue 5, 2006, Pages 1137-1141

  Kim, Byung Hyuk ^a   Oh, Hee Mock ^a   Lee, Young Ki ^a   Choi, Gang Guk ^a   Ahn, Chi Yong ^a   Yoon, Byung Dae ^a   Kim, Hee Sik ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Bead phenol chloroform method; Cyanobacteria; RNA extraction; Xanthogenate SDS phenol method

Indexed keywords

CHLOROFORM; CYANOBACTERIUM; DNA; ENZYME; LYSIS; PHENOL; RNA;

CYANOBACTERIA;

EID: 33748927801     PISSN: 00223646     EISSN: 15298817     Source Type: Journal    
DOI: 10.1111/j.1529-8817.2006.00263.x     Document Type: Article

References (20)

1. Allen, M. M. 1968. Simple conditions for growth of unicellular blue-green algae on plates. J. Phycol. 4:1-4.
2. Ausubel, F., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. 1995. Short Protocols in Molecular Biology. 3rd ed. Wiley, New York, USA, pp. 4, 10-11.
3. Bashyam, M. D. & Tyagi, A. 1994. An efficient and high-yielding method for isolation of RNA from mycobacteria. Biotechniques 17:834-6.
4. Billi, D., Caiola, M. G., Paolozzi, L. & Ghelardini, P. 1998. A method for DNA extraction from the desert cyanobacterium Chroococcidiopsis and its application to identification of ftsZ. Appl. Environ. Microbiol. 64:4053-6.
5. Cardinali, G., Liti, G. & Martina, A. 2000. Non-radioactive dot-blot DNA reassociation for unequivocal yeast identification. Int. J. Syst. Evol. Microbiol. 50:931-6.
6. Fiore, M. F., Moon, D. H., Tsai, S. M., Lee, H. & Trevors, J. T. 2000. Miniprep DNA isolation from unicellular and filamentous cyanobacteria. J. Microbiol. Methods 39:159-6.
7. Heller, M. J., Robinson, R. A., Burgart, L. J., TenEyck, C. J. & Wilke, W. W. 1992. DNA extraction by sonication: a comparison of fresh, frozen, and paraffin-embedded tissues extracted for use in polymerase chain reaction assays. Mod. Pathol. 5:203-6.
8. Holm, C., Meeks, W. D., Fangman, W. L. & Botstein, D. 1986. A rapid, efficient method for isolating DNA from yeast. Gene 42:169-73.
9. Kim, J., Park, Y., Yoon, B. & Oh, H. 1999. Establishment of axenic cultures of Anabaena flos-aquae and Aphanothece nidulans (cyanobacteria) by lysozyme treatment. J. Phycol. 35:865-9.
10. Laodie, B. M. & Ullmann, A. 1990. Virulence dependent and independent regulation of Bordetella pertussis cya operon. EMBO J. 9:999-1005.
11. Lippke, J. A., Strzempko, M. N., Raia, F. F., Simon, S. L. & French, C. K. 1987. Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate. Appl. Environ. Microbiol. 53:2588-9.
12. Madigan, M. T., Martinko, J. M. & Parker, J. 2003. Brock Biology of Microorganisms. 10th ed. Prentice Hall, New Jersey, USA, pp. 421-4.
13. Nakajima, M., Kitade, Y., Iitsuka, O., Fukuda, S. & Saga, N. 2000. Research note rapid extraction of high-quality genomic DNA from Porphyra yezoensis (Bangiales, Rhodophyta). Phycol. Res. 48:15-7.
14. Neilan, B. A., Jacobs, D. & Goodman, A. E. 1995. Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol. 61:3875-83.
15. Nuyts, S., Van Mellaert, L., Lambin, P. & Anne, J. 2001. Efficient isolation of total RNA from Clostridium without DNA contamination. J. Microbiol. Methods 44:235-8.
16. Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111:1-61.
17. Saleh-Lakha, S., Miller, M., Campbell, R. G., Schneider, K., Elahimanesh, P., Hart, M. M. & Trevors, J. T. 2005. Microbial gene expression in soil: methods, applications and challenges. J. Microbiol. Methods 63:1-19.
18. Santos, A. R., De Miranda, A. B., Sarno, E. N., Suffys, P. N. & Degrave, W. M. 1993. Use of PCR-mediated amplification of Mycobacterium leprae DNA in different types of clinical samples for the diagnosis of leprosy. J. Med. Microbiol. 39:298-304.
19. Tillett, D. & Neilan, B. A. 2000. Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria. J. Phycol. 36:251-8.
20. Varma, A. & Kwon-Chung, K. J. 1991. Rapid method to extract DNA from Cryptococcus neoformans. J. Clin. Microbiol. 29:810-2.