Valine pathway is more crucial than phenyl propanoid pathway in regulating capsaicin biosynthesis in Capsicum frutescens Mill.

Journal of Agricultural and Food Chemistry

Volumn 54, Issue 18, 2006, Pages 6660-6666

  Prasad, Bellur Chayapathy Narasimha ^a   Gururaj, Harishchandra Bhaskar ^a   Kumar, Vinod ^a   Giridhar, Parvatam ^a   Ravishankar, Gokare Ashwathnarayana ^a  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

Author keywords

8 methyl nonanoic acid; Capsaicin; Capsaicin synthase; Elicitation; Keto acyl synthase; Rhizopus oligosporus; RT PCR

Indexed keywords

8 METHYL NONENOIC ACID; 8-METHYL-NONENOIC ACID; BENZYLAMINE DERIVATIVE; CAPSAICIN; FATTY ACID; VALINE; VANILLYLAMINE;

ARTICLE; CELL CULTURE; CHEMISTRY; ENZYMOLOGY; METABOLISM; MYCELIUM; PEPPER; RHIZOPUS;

BENZYLAMINES; CAPSAICIN; CAPSICUM; CELLS, CULTURED; FATTY ACIDS; MYCELIUM; RHIZOPUS; VALINE;

ASPERGILLUS NIGER; CAPSICUM; CAPSICUM FRUTESCENS; OLIGOSPORUS; RHIZOPUS; RHIZOPUS MICROSPORUS VAR. OLIGOSPORUS;

EID: 33748891957     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf061040a     Document Type: Article

References (35)

1. Prasad, N. B. C.; Shrivastava, R.; Ravishankar, G. A. Capsaicin as multifaceted drug from Capsicum spp. Evidence-Based Int. Med. 2005, 2, 147-166.
2. Bennet, D. J.; Kirby, G. W. Constitution and biosynthesis of capsaicin. J. Chem. Soc. C 1968, 442.
3. Sukrasno, N.; Yeoman, M. M. Phenyl propanoid pathway metabolism during growth and development of Capsicum frutescens fruits. Phytochemistry 1993, 32, 839-844.
4. Ochoa-Alejo, N.; Gomez-Peralta, E. J. Activity of enzymes of involved in capsaicin biosynthesis in callus tissue and fruits of Chilli pepper (Capsicum annuum L.). J. Plant. Physiol. 1993, 141, 147-152.
5. Curry, J.; Aluru, M. R.; Mendoza, M.; Nevarez, J.; Melenderz, M.; O'Connell, M. A. Transcripts for possible capsaicinoid biosynthesis genes are differentially accumulated in pungent and non-pungent Capsicum spp. Plant Sci. 1999, 148, 47-57.
6. Kim, M.; Kim, S.; Kim, S.; Kim, B. D. Isolation of cDNA clones differentially accumulated in pungent peppers by suppression subtractive hybridization. Mol. Cells 2001, 11, 213-219.
7. Aluru, M. R.; Mazourek, M.; Landry, L. G.; Curry, J.; Jahn, M.; O'Connell, M. A. Differential expression of fatty acid synthesis genes, Acl, Fat and Kas in Capsicum fruit. J. Exp. Bot. 2003, 54, 1655-1664.
8. Pitta-Alverez, S. I.; Spollanksy, T. C.; Giuletti, A. M. The influence of abiotic and biotic elicitors on the production of and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb. Technol. 2000, 26, 252-258.
9. Brodelis, P.; Collinge, M. A.; Funk; G.; Marques, I. Studies on alkaloid formation in plant cell cultures after treatment with yeast elicitor. In Primary and Secondary Metabolism of Plant Cell Cultures; Kurz, W. G. W., Ed.; Springer-Verlag: Berlin, Germany, 1989; pp 190-199.
10. Buitelar, R. M.; Cesario, M. T.; Tamper, J. Elicitation of thiopene production by hairy root cultures of Tagetus patula. Enzyme Microb. Technol. 1992, 14, 2-7.
11. Vazquenz-Flota, F.; Moreno-Valenzuela, O.; Miranda-Ham, M. L.; Coello-Coello, J.; Loyala-Vargas, V. M. Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures, Medium optimization and elicitation. Plant Cell, Tissue Organ Cult. 1994, 38, 273-279.
12. Ciddi, V.; Srinivasan, V.; Shuler, M. L. Elicitation of Taxus sp. cell for production of taxol. Biotechnol. Lett. 1995, 17, 1343-1346.
13. Prasad, N. B. C.; Gururaj, H. B.; Kumar, V.; Giridhar, P; Parimalan, R.; Sharma, A.; Ravishankar, G. A. The influence of 8-methyl-nonenoic acid on capsaicin biosynthesis in in vivo and in vitro cell cultures of Capsicum spp. J. Agric. Food Chem. 2006, 54, 1854-1859.
14. Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 1962, 15, 473-497.
15. Johnson, T. S.; Ravishankar, G. A.; Venkataraman, L. Separation of capsaicin from phenyl propanoid compounds by high performance liquid chromatography to determine the biosynthetic status of cells and tissues of Capsicum frutescens Mill. in in vivo and in vitro. J. Agric. Food Chem. 1992, 40, 2461-2463.
16. Shimada, M.; Ohasi, H.; Higuchi, T. O-Methyltranseferases involved in the biosynthesis of lignins. Phytochemistry 1970, 9, 2463-2470.
17. Shimakata, T.; Stumf, P. K. Purification and characterization of keto acyl ACP synthatases from Spinacia oleracea leaves. Arch. Biochem. Biophys. 1983, 220, 39-45.
18. Iwai, K.; Suzuki, T.; Lee, K.; Kobashi, M.; Oka, S. In vivo and in vitro formation of dihydrocapsaicin in sweet pepper fruits, Capsicum annuum L. var. grossum. Agric. Biol. Chem. 1977, 41, 1877-1882.
19. Bradford, M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 1976, 72, 248-254.
20. Rozen, S.; Skaletsky, H. J. Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols, Methods in Molecular Biology; Krawetz, S. M. S., Ed.; Humana Press: Totowa, NJ, 2000; pp 365-386.
21. Tukey, J. W. Some selected quick and easy methods of statistical analysis. Trans. N.Y. Acad. Sci., Ser. II 1953, 16, 88-97.
22. Hahlbrock, K.; Bednarek, P.; Ciolkowski, I.; Hamberger, B.; Heise, A.; Liedgens, H.; Logemann, E.; Nürnberger, T.; Somssich, I. E.; Tan, J. Non-self recognition, transcriptional reprogramming, and secondary metabolite accumulation during plant/pathogen interactions. Proc. Natl. Acad. Sci. U.S.A. 2003, 25 (supplement 2), 14569-14576.
23. Dixon, R. A. Natural products and plant disease resistance. Nature 2001, 411, 843-847.
24. Tan, J.; Schneider, B.; Svatos, A.; Bednarek, P.; Liu, J.; Hahlbrock, K. Universally occurring phenyl propanoid and species-specific indolic metabolites in infected and uninfected Arabidopsis thaliana roots and leaves. Phytochemistry 2004, 65, 691-699.
25. Matamoros, L.; Argaiz, A.; Lopez, M. A. Individual and combined effects of vanillin and potassium sorbate on Penicillium digitatum and P. glabrum growth. J. Food Prot. 1999, 62, 540-542.
26. Zaldivar, J.; Martinez, A.; Ingram, L. O. Effect of selected aldehydes on the growth and fermentation of ethanologenic E. coli. Biotechnol. Bioeng. 1999, 65, 24-33.
27. Overhage, J.; Kresse, A. U.; Priefert, H.; Sommer, H.; Krammer, G.; Rabenhorst, J.; Steinbuchel, A. Molecular characterization of the genes pcaG and pcaH encoding protocatechuate 3,4-dioxygenase which are essential for vanillin catabolism in Pseudomonas spp. strain HR199. Appl. Environ. Microbiol. 1999, 65, 951-960.
28. Priefert, H.; Rabenhorst, J.; Steinbuchel, A. Biotechnological production of vanillin. Appl. Microbiol. Biotechnol. 2001, 56, 296-314.
29. Valton, N. J.; Mayer, M. J.; Narbad, A. Molecules of interest - Vanillin. Phytochemistry 2003, 63, 505-515.
30. Walsh, B. M.; Hoot, S. B. Phylogenetic relationships of Capsicum (Solanaceae) using DNA sequences from two noncoding regions: The chloroplast atpB-rbcL spacer region and nuclear waxy introns. Int. J. Plant Sci. 2001, 162, 1409-1418.
31. Andrews, J. Peppers: The Domesticated Capsicums; University of Texas Press: Austin, TX, 1984.
32. Choi, D.; Bostock, R. M.; Avdiushko, S.; Hildebrand, D. F. Lipid derived signals that discriminate wound and pathogen responsive isoprenoid pathway in plants. Methyl jasmonate and fungal elicitor arachidonic acid induce different 3-hydroxy-3-methyl glutaryl CoA reductase genes and antimicrobial isoprenoids in Solanum tuberosum L. Proc. Natl. Acad. Sci. U.S.A. 1994, 41, 2329-2333.
33. Menke, F. L. H.; Parchmann, S.; Mueller, M. J.; Kijne, J. W.; Memelink, J. Involvement of the octadecanoid pathway and protein phosphorylation in fungal elicitor induced expression of terpenoid indole alkaloid biosynthesis genes in Catharanthus roseus. Plant Physiol 1999, 119, 1289-1296.
34. Ellard-Ivey, M.; Douglas, C. J. Role of jasmonates in the elicitor and wound inducible expression of defense genes in parsley and transgenic tobacco. Plant Physiol. 1996, 112, 183-192.
35. Prasad, N. B. C.; Giridhar, P.; Kumar, V.; Ravishankar, G. A. A spray formulation for pungency enhancement in Capsicum fruits. Patent Pending U.S. application number 10/955786, PCT application number PCT/IB04/03197, 2003.