Metabolic changes in mesenchymal stem cells in osteogenic medium measured by autofluorescence spectroscopy

Stem Cells

Volumn 24, Issue 5, 2006, Pages 1213-1217

  Reyes, Johann M G ^a   Fermanian, Sara ^b   Yang, Fan ^b   Zhou, Shi You ^a   Herretes, Samantha ^a   Murphy, Douglas B ^c   Elisseeff, Jennifer H ^b   Chuck, Roy S ^a,d  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b Johns Hopkins University   (United States)

^c JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

Bone marrow; Differentiation; Fluorescence microscopy; Mesenchymal stem cells

Indexed keywords

FLAVOPROTEIN; PYRIDINE;

ANIMAL CELL; ARTICLE; AUTOFLUORESCENCE; BONE DEVELOPMENT; CELL CONTACT; CELL DENSITY; CELL DIFFERENTIATION; FLUOROMETRY; MESENCHYMAL STEM CELL; METABOLIC DISORDER; NONHUMAN; OXIDATION REDUCTION REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; BIOLOGICAL MARKERS; CELL PROLIFERATION; CELLS, CULTURED; CORE BINDING FACTOR ALPHA 1 SUBUNIT; CULTURE MEDIA, CONDITIONED; GOATS; MESENCHYMAL STEM CELLS; OSTEOGENESIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPECTROMETRY, FLUORESCENCE;

EID: 33748806476     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2004-0324     Document Type: Article

References (25)

1. Jaiswal RK, Jaiswal N, Bruder SP et al. Adult human mesenchymal stem cell differentiation to the osteogenic or adipogenic lineage is regulated by mitogen-activated protein kinase. J Biol Chem 2000;275:9645-9652.
2. Ryden M, Dicker A, Gotherstrom C et al. Functional characterization of human mesenchymal stem cell-derived adipocytes. Biochem Biophys Res Commun 2003;311:391-397.
3. Solchaga LA, Welter JF, Lennon DP et al. Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotype. Methods Mol Med 2004;100:53-68.
4. Meinel L, Karageorgiou V, Fajardo R et al. Bone tissue engineering using human mesenchymal stem cells: Effects of scaffold material and medium flow. Ann Biomed Eng 2004;32:112-122.
5. Qian L, Saltzman WM. Improving the expansion and neuronal differentiation of mesenchymal stem cells through culture surface modification. Biomaterials 2004;25:1331-1337.
6. Luo Y, Cai J, Ginis I et al. Designing, testing and validating a focused stem cell microarray for characterization of neural stem cells and progenitor cells. STEM CELLS 2003;21:575-587.
7. Bradford GB, Williams B, Rossi R et al. Quiescence, cycling, and turnover in the primitive hematopoietic stem cell compartment. Exp Hematol 1997;25:445-453.
8. Masters BR, Ghosh AK, Wilson J et al. Pyridine nucleotides and phosphorylation potential of rabbit corneal epithelium and endothelium. Invest Ophthalmol Vis Sci 1989;30:861-868.
9. Rocheleau JV, Head WS, Piston DW. Quantitative NAD(P)H/flavoprotein autofluorescence imaging reveals metabolic mechanisms of pancreatic islet pyruvate response. J Biol Chem 2004;279:31780-31787.
10. Tsubota K, Laing RA, Chiba K et al. Noninvasive metabolic analysis of preserved rabbit cornea. Arch Ophthalmol 1988;106:1713-1717.
11. Piston DW, Masters BR, Webb WW. Three-dimensionally resolved NAD(P)H cellular metabolic redox imaging of the in situ cornea with two photon excitation laser scanning microscopy. J Microsc 1995;178:20-27.
12. Yeh AT, Nassif N, Zoumi A et al. Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence. Opt Lett 2002;27:2082-2084.
13. Zoumi A, Yeh A, Tromberg BJ. Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence. Proc Natl Acad Sci U S A 2002;99:11014-11019.
14. Croce AC, Spano A, Locatelli D et al. Dependence of fibroblast autofluorescence properties on normal and transformed conditions. Role of metabolic activity. Photochem Photobiol 1999;69:364-374.
15. Zhang JC, Savage HE, Sacks PG et al. Innate cellular fluorescence reflects alterations in cellular proliferation. Lasers Surg Med 1997;20:319-331.
16. Smith J, Ladi E, Mayer-Proschel M et al. Redox state is a central modulator of the balance between self-renewal and differentiation in a dividing glial precursor cell. Proc Natl Acad Sci U S A 2000;97:10032-10037.
17. Williams CG, Kim TK, Taboas A et al. In vitro chondrogenesis of bone-marrow derived mesenchymal stem cells in a photopolymerizing hydrogel. Tissue Engineering 2003;9:679-688.
18. Jaiswal N, Haynesworth SE, Caplan AI et al. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 1997;64:295-312.
19. Murphy JM, Fink DJ, Hunziker EB et al. Stem cell therapy in a caprine model of osteoarthritis. Arthritis Rheum 2003;48:3464-3474.
20. Pittenger MF, Mackay AM, Beck SC et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143-147.
21. Barry FP, Murphy JM. Mesenchymal stem cells: Clinical applications and biological characterization. Int J Biochem Cell Biol 2004;36:568-584.
22. Yang F, Williams CG, Wang D et al. The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells. Biomaterials 2005;26:5991-5998.
23. McBeath R, Pirone DM, Nelson CM et al. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 2004;6:483-495.
24. Guo Y, Einhorn L, Kelley M et al. Redox regulation of the embryonic stem cell transcription factor Oct-4 by Thioredoxin. STEM CELLS 2004;22:259-264.
25. Bereiter-Hahn J, Munnich A, Woiteneck P. Dependence of energy metabolism on the density of cells in culture. Cell Struct Funct 1998;23:85-93.