In vitro antioxidative activity of pumpkin seed (Cucurbita pepo) protein isolate and its in vivo effect on alanine transaminase and aspartate transaminase in acetaminophen-induced liver injury in low protein fed rats

Phytotherapy Research

Volumn 20, Issue 9, 2006, Pages 780-783

  Nkosi, C Z ^a   Opoku, A R ^a   Terblanche, S E ^a  

^a UNIVERSITY OF ZULULAND   (South Africa)

Author keywords

Acetaminophen induced liver injury; Antioxidative properties; Cucurbita pepo; Low protein fed rats; Plasma enzymes; Pumpkin seed protein isolate

Indexed keywords

ALANINE AMINOTRANSFERASE; ASPARTATE AMINOTRANSFERASE; IRON; PARACETAMOL; POLYPHENOL; SCAVENGER; THIOL DERIVATIVE; VEGETABLE PROTEIN;

ANIMAL EXPERIMENT; ARTICLE; CHELATION; CONTROLLED STUDY; DRUG ACTIVITY; DRUG EFFECT; DRUG INTOXICATION; IN VITRO STUDY; IN VIVO STUDY; LIVER INJURY; MALE; NONHUMAN; PROTEIN ISOLATION; PROTEIN RESTRICTION; RAT; SQUASH;

ALANINE TRANSAMINASE; ANIMALS; ANTIOXIDANTS; ASPARTATE AMINOTRANSFERASES; CUCURBITA; FLAVONOIDS; HEPATITIS, ANIMAL; IRON CHELATING AGENTS; MALE; PHENOLS; PLANT EXTRACTS; PROTEIN DEFICIENCY; RATS; RATS, SPRAGUE-DAWLEY; SEEDS; SULFHYDRYL COMPOUNDS; TRANSAMINASES; XANTHINE OXIDASE;

CUCURBITA PEPO;

EID: 33748800713     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.1958     Document Type: Article

References (20)

1. Black M. 1984. Acetaminophen hepatotoxicity. Ann Rev Med 35: 577-593.
2. Cohen VH, Lyle J. 1966. A fluorometric assay for glutathione. Anal Biochem 14: 434-440.
3. Constantino L, Albasino A, Rastelli G, Benvenuti S. 1992. Activity of polyphenolics crude extracts on scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Med 58: 342-344.
4. Dahlin DC, Miwa GT, Lu AYH, Nelson SD. 1984. N-acetyl-p-benzoquinone imine: A cytochrome P-450-dependent oxidation product of acetaminophen. Proc Natl Acad Sci 18: 1327-1331.
5. Decker EA, Welch B. 1990. Role of ferritin as a lipid oxidation catalyst in muscle food. J Agric Food Chem 38: 674-677.
6. Golden MNH, Ramdath D. 1987. Free radicals in the pathogenesis of kwashiorkor. Proc Nutr Soc 46: 53-68.
7. Halliwell B. 1994. Free radicals and antioxidants. Lancet 344: 721-724.
8. Hoffmann KJ, Streeter AJ, Axworth DB, Baillie TA. 1985. Structural characterization of the major covalent adduct formed in vitro and in vivo between acetaminophen and mouse liver proteins. Mol Pharmacol 27: 566-573.
9. Hørder M, Rej R. 1983. Alanine aminotransferase. In Methods of Enzymatic Analysis, 3rd edn, vol 3, Bergmeyer HU (ed.). Verlag Chemie: Weinheim, 444-450.
10. Lin S-C, Yao C-J, Lin C-C, Lin Y-H. 1996. Hepatoprotective activity of Taiwan folk medicine: Eclipta prostata Linn. against various hepatotoxins induced acute hepatotoxicity. Phytother Res 10: 483-490.
11. McLean AEM. 1962. Hepatic failure in malnutrition. Lancet 2: 1229-1295.
12. Mitra SK, Venkatarangama MV, Sunderam R, Gopumadhavan S. 1998. Effect of HD-03, a herbal formulation, on the anti-oxidant defense system in rats. Phytother Res 12: 114-117.
13. Navarro MC, Montilla MP, Martin A, Jimenez J, Utrilla MP. 1992. Free radicals and antihepatotoxic activity of Rosmarinus tomentosus. Planta Med 59: 312-314.
14. Nemec A, Drobnič-Košorok M, Skitek M, Pavlica Z, Galac S, Butinar J. 2000. Total antioxidant capacity (TAC) values and their correlation with individual antioxidants in serum of healthy beagles. Acta Vet Brno 69: 297-303.
15. 4-induced liver injury in low-protein fed rats. Phytother Res 19: 341-345.
16. Opoku AR, Maseko NF, Terblanche SE. 2002. The in vitro antioxidative activity of some traditional Zulu medicinal plants. Phytother Res 16: S51-S56.
17. Potter DW, Hinson JA. 1986. Reactions of N-acetyl-p-benzoquinone imine with reduced glutathione, acetaminophen, and NADPH. Mol Pharmacol 30: 33-41.
18. Rej R, Hørder M. 1983. Aspartate aminotransferase. In Methods of Enzymatic Analysis, 3rd edn, vol 3, Bergmeyer HU (ed.). Verlag Chemie: Weinheim, 418-424.
19. Sodini G, Canella M. 1977. Acidic butanol removal of color-forming phenols from sunflower meal. J Agric Food Chem 25: 822-825.
20. Swain T, Hillis WE. 1959. Phenolic constituents of Prunus domestica. J Sd Food Agric 10: 63-68.