Reduced expression of the connexin26 gene and its aberrant DNA methylation in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine

Molecular Carcinogenesis

Volumn 45, Issue 9, 2006, Pages 710-714

  Shimizu, Kyoko ^a   Shimoichi, Yozo ^a   Hinotsume, Daisei ^a   Itsusaki, Yumi ^a   Fuji, Hiromasa ^b   Honoki, Kanya ^b   Tsujiuchi, Toshifumi ^a  

^a KINKI UNIVERSITY   (Japan)

^b NARA MEDICAL UNIVERSITY   (Japan)

Author keywords

Connexin26; DNA methylation; Lung adenocarcinoma; Rat

Indexed keywords

BIS(2 HYDROXYPROPYL)NITROSAMINE; CONNEXIN 26; GAP JUNCTION PROTEIN; ION CHANNEL; TUMOR SUPPRESSOR PROTEIN;

5' UNTRANSLATED REGION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA METHYLATION; GAP JUNCTION; GENE EXPRESSION; LUNG ADENOCARCINOMA; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FAMILY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SEQUENCE ANALYSIS;

ADENOCARCINOMA; ANIMALS; CARCINOMA, NON-SMALL-CELL LUNG; CONNEXINS; DNA METHYLATION; GENE EXPRESSION REGULATION, NEOPLASTIC; LUNG NEOPLASMS; MALE; NITROSAMINES; RATS; RATS, WISTAR;

RATTUS NORVEGICUS;

EID: 33748789834     PISSN: 08991987     EISSN: 10982744     Source Type: Journal    
DOI: 10.1002/mc.20207     Document Type: Article

References (28)

1. Kumar NM, Gilula NB. The gap junction communication channel. Cell 1996;84:381-388.
2. Yamasaki H, Naus CC. Role of Connexin genes in growth control. Carcinogenesis 1996;17:1199-1213.
3. Bruzzone R, White TW, Paul DL. Connections with Connexins: The molecular basis of direct intercellular signaling, Eur J Biochem 1996;238:1-27.
4. Locke D. Gap junctions in normal and neoplastic mammary glands. J Pathol 1998;186:343-349.
5. Trosko JE, Ruch RJ. Cell-cell communication in carcinogenesis. Front Biosci 1998;3:D208-D236.
6. Laird DW, Fistouris P, Batist G, et al. Deficiency of Connexin 43 gap junctions is an independent marker for breast tumours. Cancer Res 1999;59:4101-4110.
7. Hirschi KK, Xu C, Tsukamoto T, Sager R. Gap junction genes Cx26 and Cx43 individually suppress the cancer phenotype of human mammary carcinoma cells and restore differentiation potential. Cell Growh Diff 1996;7:861-870.
8. Muramatu A, Iwai M, Morikawa T, et al. Influence of transfection with Connexin 26 gene in malignant potential of human hepatoma cells. Carcinogenesis 2002;23:351-358.
9. Bry C, Maass K, Miyoshi K, et al. Loss of Connexin 26 in mammary epithelium during early nut not during late pregnancy results in unscheduled apoptosis and impaired development. Dev Biol 2004;267:418-429.
10. Li GY, Lin HH, Zheng JT, Kiang DT. Gapjunction Cx26 gene modulation by phorbal ester in benign and malignant human mammary cells. Gene 1998;209:139-147.
11. Chen Y, Huhn D, Knosel T, et al. Downregulation of Connexin 26 in human lung cancer is related to promoter methylation. Int J Cancer 2005;113:14-21.
12. Tan LW, Bianco T, Dobrovic A. Variable promoter region CpG island methylation of the putative tumor suppressor gene Connexin 26 in breast cancer. Carcinogenesis 2002;23:231-236.
13. Hellman P, Grmmer R, Schirrmacher K, et al. Transfection with different Connexin genes alters growth and differentiation of human choriocarcinoma cells. Exp Cell Res 1999; 246:480-490.
14. Konishi Y, Denda A, Kondo H. Takahashi S. Lung carcinomas induced by oral administration of N-bis(2-hydroxypropyl)nitrosamine in rats. Jpn J Cancer Res 1976;67:773-780.
15. Konishi Y, Kondo H, Denda A, Takahashi S, Inui S. Lung carcinomas induced by oral administration of N-bis (2-hydroxypropyl)nitrosamine in rats. In: Severi I, editor. Tumors of Early Life in Man and Animals. Perugia Quandernnaial International Conference of Cancer. Perugia: Division of Cancer Research, Perugia University, 1978. p 637-649.
16. Kitada H, Tsutsumi M, Tsujiuchi T, et al. Frequent mutations of Ki-ras but no mutations of Ha-ras and p53 in lung lesions induced by N-nitrosobis(2-hydroxypropyl)amine in rats. Mol Carcinog 1996;15:276-283.
17. Tsujiuchi T, Sasaki Y, Tsustumi M, Konishi Y. Mutations of the smad2 and smad4 genes in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl) amine in rats. Mol Carcinog 2000;29:87-91.
18. Tsujiuchi T, Sasaki Y, Tsustumi M, Konishi Y. Mutations and reduced expression of the transforming growth factor-β receptor II gene in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine. Jpn J Cancer Res 2000; 91:1090-1095.
19. Tsujiuchi T, Sasaki Y, Murata N, et al. FHIT alterations in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in rats. Carcinogenesis 2001;22:2017-2022.
20. Tsujiuchi T, Sasaki Y, Oka Y, et al. Alterations of the Dutt1/Robo1 gene in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine in rats. Mol Carcinog 2004;40:241-246.
21. Shimizu K, Kato A, Hinotsume D, et al. Reduced expressions of Foxp1 and Rassf1a genes in lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl) amine in rats. Cancer Lett 2006; in press.
22. Shimizu K, Kato A, Shigemura M, et al. Aberrant methylation patterns of the Rassf1a gene in rat lung adenocarcinomas induced by N-nitrosobis(2- hydroxypropyl)amine. Mol Carcinog 2006;45:112-117.
23. Kato A, Shimizu K, Shimoichi Y, et al. Aberrant DNA methylation of E-cadherin and p16 genes in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine. Mol Carcinog 2006;45:106-111.
24. Cohen-Salmon M, Ott T, Michel V, et al. Targeted ablation of Connexin26 in the inner ear epithelial gap junction network causes hearing impairment and cell death. Curr Biol 2002; 12:1106-1111.
25. Nishimura M, Saito T, Yamasaki H, Kudo R. Suppression of gap junctonal intercellular communication via 5′ CpG island methylation in promoter region of E-cadherin gene in endometrial cancer cells. Carcinogenesis 2003;24:1615-1623.
26. Takeichi M. Cadherin cell adhesion receptor as a morphogenetic regulator. Science 1991;251:1451-1455.
27. Yoshimurta K, Kanai Y, Ochiai A, et al. Silencing of the E-cadherin invasion-suppressor gene by CpG methylation in human carcinomas. Proc Natl Acad Sci USA 1995;92:7416-7419.
28. Kanai Y, Ushijima S, Hui AM, et al. The E-cadherin gene is silenced by CpG methylation in human hepatocellular carcinomas. Int J Cancer 1997;71:355-359.