Development of an HPLC-PAD-MS assay for the identification and quantification of major phenolic edelweiss (Leontopodium alpium Cass.) constituents

Phytochemical Analysis

Volumn 17, Issue 5, 2006, Pages 291-298

  Schwaiger, Stefan ^a   Seger, Christoph ^a   Wiesbauer, Bettina ^a   Schneider, Peter ^a   Ellmerer, Ernst P ^a   Sturm, Sonja ^a   Stuppner, Hermann ^a  

^a UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Asteraceae; Edelweiss; Glucaric acid; HPLC PAD MS; Leontopodic acid; Leontopodium alpinum

Indexed keywords

ANTENNAS; CARBOHYDRATES; COLUMN CHROMATOGRAPHY; FLAVONOIDS; MASS SPECTROMETRY; PLANTS (BOTANY);

ANALYTES; APIGENIN; ASTERACEAE; EDELWEISS; GLUCARIC ACID; HPLC PADS; HPLC-PAD-MS; LEONTOPODIC ACID; LEONTOPODIUM ALPINUM; NATURAL PRODUCTS;

NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

3,5 DICAFFEOYLYQUINIC ACID; 6 HYDROXYLUTEOLIN 7 O BETA DEXTRO GLUCOSIDE; APIGENIN; APIGENIN 7 O BETA DEXTRO GLUCOSIDE; CHLOROGENIC ACID; CHRYSOERIOL 7 O BETA DEXTRO GLUCOSIDE; LEONTOPODIC ACID; LEONTOPODIC ACID B; LEONTOPODIUM ALPIUM EXTRACT; LUTEOLIN; LUTEOLIN 3' O BETA DEXTRO GLUCOSIDE; LUTEOLIN 4' O BETA DEXTRO GLUCOSIDE; LUTEOLIN 7 O BETA DEXTRO GLUCOSIDE; LUTEOLIN 7,4' DI O BETA DEXTRO GLUCOSIDE; NATURAL PRODUCT; PHENOL DERIVATIVE; PLANT EXTRACT; QUERCETIN 3 O BETA DEXTRO GLUCOSIDE; UNCLASSIFIED DRUG;

ARTICLE; ASTERACEAE; COLUMN CHROMATOGRAPHY; DRUG IDENTIFICATION; DRUG ISOLATION; DRUG STRUCTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LEONTOPODIUM ALPIUM; MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PLANT LEAF; PLANT STEM; QUANTITATIVE ANALYSIS; SEPARATION TECHNIQUE; ULTRAVIOLET SPECTROSCOPY;

ASTERACEAE; LEONTOPODIUM; LEONTOPODIUM ALPINUM;

EID: 33748782751     PISSN: 09580344     EISSN: 10991565     Source Type: Journal    
DOI: 10.1002/pca.917     Document Type: Article

References (15)

1. Di Modica G, Tira S. 1963. Chromatographic comparison of flavonoid pigments from Inuleae. Ann Chim 53: 764-773.
2. Dobner MJ, Schwaiger S, Jenewein IH, Stuppner H. 2003. Antibacterial activity of Leontopodium alpinum (edelweiss). J Ethnopharmac 89: 301-303.
3. Handl-Mazzetti H. 1928. Systematische Monographie der Gattung Leontopodium. Beih Bot Centralbl 44: 1-178.
4. Hennessy D, Hook I, Sheridan H, McGee A. 1989. Hydroxycinnamic acid esters from cell suspension cultures and plants of Leontopodium alpinum. Phytochemistry 28: 489-490.
5. Kellam SJ, Mitchell KA, Blunt JW, Munro MHG, Walker JRL. 1993. Luteolin and 6-hydroxyluteoline glycosides from Hebe stricta. Phytochemistry 33: 867-869.
6. Lewis DH, Bloor SJ, Schwinn KE, 1998. Flavonoid and carotenoid pigments in flower tissue of Sandersonia aurantiaca Hook. Scientia Horticulturae 72: 179-192.
7. Li S, Fritz JS. 2002. Organic modifiers for the separation of organic acids and bases by liquid chromatography. J. Chromatogr. A 964: 91-98.
8. Nawwar MAM, Hussein SAM, Merfort I. 1994. Leaf phenolics of Punica granatum. Phytochemistry 37: 1175-1177.
9. Pauli GF, Poetsch F, Nahrstedt A. 1998. Structure assignment of natural quinic acid - derivatives using proton nuclear magnetic resonance techniques. Phytochem. Anal. 9: 177-185.
10. Pieroni A, Heimler D, Pieters L, van Poel B, Vlietinck AJ. 1996. In vitro anti-complementary activity of flavonoids from olive (Olea europae L.) leaves. Pharmazie 51: 765-768.
11. Schwaiger S, Cervellati R, Seger C, Ellmerer EP, About N, Renimel I, Godenir C, Andréd P, Gafner F, Stuppner H. 2005. Leontopodic acid - a novel highly substituted glucaric acid derivative from edelweiss (Leontopodium alpinum Cass.) and its antioxidative and DNA protecting properties. Tetrahedron 61: 4621-4630.
12. 13C NMR signal assignment of benzylisoquinoline alkaloids from Fumaria officinalis L. (Papaveraceae). Magn. Reson. Chem. 42: 882-886.
13. Slimestad R, Andersen OM, Francis GW, Marston A, Hostettmann K. 1995. Syringentin-3-O-(6″-acetyl)-β-glucopyranoside and other flavonols from the needles of Norway spruce, Picea abies. Phytochemistry 40: 1537-1542.
14. Waksmundzka-Hajnos M. 1998. Chromatographic separations of aromatic carboxylic acids. J. Chromatogr. B 717: 93-118.
15. Yinrong L, Yeap FL. 2000. Flavonoid and phenolic glycosides from Salvia offcinalis. Phytochemistry 55: 263-267.