Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by clostridium difficile

Applied and Environmental Microbiology

Volumn 72, Issue 9, 2006, Pages 6062-6069

  Kim, Jihoe ^a   Darley, Daniel ^a   Selmer, Thorsten ^a   Buckel, Wolfgang ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; BACTERIA; CATALYSIS; FERMENTATION; MUTAGENESIS; REDUCTION; SUBSTRATES;

ANHYDRIDES; CATALYTIC RESIDUE; COA TRANSFERASE; THIOESTER SUBSTRATES;

ENZYME KINETICS;

2 HYDROXY 4 METHYLVALERATE DEHYDROGENASE; 2 OXOISOCAPROIC ACID; AMINO ACID DERIVATIVE; COENZYME A TRANSFERASE; ENZYME INHIBITOR; HYBRID PROTEIN; HYDROXYLAMINE; ISOCAPROATE; LEUCINE; OXIDOREDUCTASE; PROTEIN LDHA; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; SODIUM BOROHYDRIDE; THIOESTER; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; ENZYME ACTIVITY; FECAL COLIFORM; FERMENTATION;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENOME; CLOSTRIDIUM DIFFICILE; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE OVEREXPRESSION; HADA GENE; LDHA GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN FAMILY; SITE DIRECTED MUTAGENESIS;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYTIC DOMAIN; CLONING, MOLECULAR; CLOSTRIDIUM DIFFICILE; COENZYME A-TRANSFERASES; DNA, BACTERIAL; GENES, BACTERIAL; HEXANOIC ACIDS; HUMANS; KINETICS; LEUCINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM DIFFICILE; ESCHERICHIA COLI;

EID: 33748771961     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00772-06     Document Type: Article

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