Ca2+-binding allergens from olive pollen exhibit biochemical and immunological activity when expressed in stable transgenic Arabidopsis

FEBS Journal

Volumn 273, Issue 19, 2006, Pages 4425-4434

  Ledesma, Amalia ^a   Moral, Verónica ^b   Villalba, Mayte ^a   Salinas, Julio ^b   Rodríguez, Rosalía ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b INIA   (Spain)

Author keywords

Allergen; Ole e 3; Ole e 8; Olive pollen; Plant expression

Indexed keywords

ANTISERUM; COMPLEMENTARY DNA; IMMUNOGLOBULIN G; OLE E 3 POLLEN; OLE E 3 RECOMBINANT PROTEIN; OLE E 8 POLLEN; OLE E 8 RECOMBINANT PROTEIN; POLLEN ANTIGEN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; ALLERGEN; CALCIUM; CALCIUM BINDING PROTEIN; OLE E 8 PROTEIN, OLIVE; OLE3 PROTEIN, OLEA EUROPAEA; VEGETABLE PROTEIN;

ARABIDOPSIS; ARTICLE; CALCIUM BINDING; CHEMICAL ANALYSIS; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ESCHERICHIA COLI; GENOME; IMMUNOREACTIVITY; MASS SPECTROMETRY; NONHUMAN; OLIVE; PLANT GENETICS; POLLEN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN CONFORMATION; TRANSGENIC PLANT; AMINO ACID SEQUENCE; BIOSYNTHESIS; CHEMISTRY; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; OLIVE TREE;

ARABIDOPSIS; ARABIDOPSIS THALIANA; ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS; PICHIA PASTORIS;

ALLERGENS; AMINO ACID SEQUENCE; ARABIDOPSIS; CALCIUM; CALCIUM-BINDING PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; OLEA; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; POLLEN; RECOMBINANT PROTEINS;

EID: 33748747665     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2006.05417.x     Document Type: Article

References (38)

1. Kay AB (1997) Concepts of allergy and hypersensitivity. Allergy and Allergic Diseases (Kay AB, ed.), p. 24. Blackwell, Oxford.
2. Valenta R, Ball T, Focke M, Linhart B, Mothes N, Niederberger V, Spitzauer S, Swoboda I, Vrtala S, Westritschnig K et al. (2004) Immunotherapy of allergic disease. Adv Immunol 82, 105-153.
3. Bousquet J, Lockey R & Malling HJ (1998) Allergen immunotherapy: therapeutic vaccines for allergic diseases. A WHO position paper. J Allergy Clin Immunol 102, 558-562.
4. Valenta R & Kraft D (2004) Recombinant allergens: from production and characterization to diagnosis, treatment, and prevention of allergy. Methods 32, 207-208.
5. Ma JK, Drake PM & Christou P (2003) The production of recombinant pharmaceutical proteins in plants. Nat Rev Genet 4, 794-805.
6. Streatfield SJ & Howard JA (2003) Plant-based vaccines. Int J Parasitol 33, 479-493.
7. Krebitz M, Wiedermann U, Essl D, Steinkellner H, Wagner B, Turpen TH, Ebner C, Scheiner O & Breiteneder H (2000) Rapid production of the major birch pollen allergen Bet v, 1 in Nicotiana benthamiana plants and its immunological in vitro and in vivo characterization. FASEB J 14, 1279-1288.
8. Krebitz M, Wagner B, Ferreira F, Peterbauer C, Campillo N, Witty M, Kolarich D, Steinkellner H, Scheiner O & Breiteneder H (2003) Plant-based heterologous expression of Mal d 2, a thaumatin-like protein and allergen of apple (Malus domestica), and its characterization as an antifungal protein. J Mol Biol 329, 721-730.
9. Breiteneder H, Krebitz M, Wiedermann U, Wagner B, Essl D, Steinkellner H, Turpen TH, Ebner C, Buck D, Niggemann B et al. (2001) Rapid production of recombinant allergens in Nicotiana benthamiana and their impact on diagnosis and therapy. Int Arch Allergy Immunol 124, 48-50.
10. 2-mediated responses. Proc Natl Acad Sci USA 102, 17525-17530.
11. Bousquet J, Cour P, Guerin B & Michel FB (1985) Allergy in Mediterranean area. I. Pollen counts and pollinosis in Montpellier. Clin Allergy 14, 249-258.
12. Macchia L, Caiaffa MF, D'Amato G & Tursi A (1991) Allergenic significance of Oleaceae pollen. In Allergic Pollen and Pollinosis in Europe (D'Amato G, Spieksma FThM & Bonini S, eds), pp. 87-93. Blackwell Scientific, Oxford.
13. Rodríguez R, Villalba M, Batanero E, González EM, Monsalve RI, Huecas S, Tejera ML & Ledesma A (2002) Allergenic diversity of the olive pollen. Allergy 57 (Suppl. 71), 6-16.
14. Barral P, Batanero E, Palomares O, Quiralte J, Villalba M & Rodríguez R (2004) A major allergen from pollen defines a novel family of plant proteins and shows intra- and interspecies cross-reactivity. J Immunol 172, 3644-3651.
15. Batanero E, Villalba M, Ledesma A, Puente XS & Rodríguez R (1996) Ole e 3, an olive-tree allergen, belongs to a widespread family of pollen proteins. Eur J Biochem 241, 772-778.
16. 2+-binding proteins (polcalcins) involved in allergy. Eur J Biochem 258, 454-459.
17. Valenta R, Hayek B, Seiberler S, Bugajska-Schretter A, Niederberger V, Twardosz A, Natter S, Vangelista L, Pastore A, Spitzauer S et al. (1998) Calcium-binding allergens: from plants to man. Int Arch Allergy Immunol 117, 160-166.
18. 2+-binding protein from olive pollen with allergenic activity. FEBS Lett 466, 192-196.
19. Ledesma A, Villalba M, Vivanco F & Rodríguez R (2002) Olive pollen allergen Ole e 8: identification in mature pollen and presence of Ole e 8-like proteins in different pollens. Allergy 57, 40-43.
20. Baulcombe DC, Saunders GR, Bevan MW, Mayo MA & Harrison BD (1986) Expression of biologically active viral satelite RNA from nuclear genome of transformed plants. Nature 321, 446-449.
21. Gómez N, Carrillo C, Salinas J, Parra F, Borca MV & Escribano JM (1998) Expression of immunogenic glycoprotein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants. Virology 249, 352-358.
22. Gil F, Brun A, Wigdorovitz A, Catala R, Martínez-Torrecuadrada JL, Casal I, Salinas J, Borca MV & Escribano JM (2001) High-yield expression of a viral peptide vaccine in transgenic plants. FEBS Lett 488, 13-17.
23. Ikura M (1996) Calcium binding and conformational response in EF-hand proteins. Trends Biochem Sci 21, 14-17.
24. 2+-binding motifs involved in the immunoglobulin E-binding of allergens? Olive pollen allergens as model of study. Clin Exp Allergy 32, 1476-1483.
25. Tacket CO, Mason HS, Losonsky G, Estes MK, Levine MM & Arntzen CJ (2000) Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes. J Infect Dis 182, 302-305.
26. Lamphear BJ, Streatfield SJ, Jilka JM, Brooks CA, Barker DK, Turner DD, Delaney DE, Garcia M, Wiggins B, Woodard SL et al. (2002) Delivery of subunit vaccines in maize seed. J Control Release 85, 169-180.
27. Streatfield SJ, Lane JR, Brooks CA, Barker DK, Poage ML, Mayor JM, Lamphear BJ, Drees CF, Jilka JM, Hood EE et al. (2003) Corn as a production system for human and animal vaccines. Vaccine 21, 812-815.
28. low T cell-dependent mechanism. J Immunol 171, 2116-2126.
29. Hsu C, Lin S, Liu F, Su W & Yeh S (2004) Oral administration of a mite allergen expressed by zucchini yellow mosaic virus in cucurbit species downregulates allergen-induced airway inflammation and IgE synthesis. J Allergy Clin Immunol 113, 1079-1185.
30. Haughn G & Somerville C (1986) Sulfonylurea-resistant mutants of Arabidopsis thaliana. Mol Gen Genet 204, 430-434.
31. Deblaere R, Bytebier B, De Greve H, Deboeck F, Schell J, Van Montagu M & Leemans J (1985) Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Res 13, 4777-4788.
32. Clough SJ & Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16, 735-743.
33. Murashige Y & Skoog F (1982) A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol 15, 473-497.
34. Logeman J, Schell J & Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163, 16-20.
35. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring. Harbor Laboratory Press, Cold Spring Harbor, NY.
36. Lowry OH, Rosebrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-275.
37. Sechi S & Chait BT (1998) Modification of cysteine residues by alkylation. A tool in peptide mapping and protein identification. Anal Chem 70, 5150-5158.
38. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.