A capillary cytometer method to quantitate viable virus particles based on early detection of viral antigens and cellular events within single cells

Journal of Virological Methods

Volumn 137, Issue 2, 2006, Pages 213-218

  Pheasey, Nigel ^a,b   John, Justin ^b   Love, Colin ^b   Coffin, Rob ^a,b   Ward, John M ^a   Boushaba, Rihab ^a   Hoare, Mike ^a   Levy, M Susana ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b BioVex Ltd   (United Kingdom)

Author keywords

Capillary cytometer; Infectivity; Plaque forming units; Viable virus quantitation

Indexed keywords

POLYCLONAL ANTIBODY; VIRUS ANTIGEN;

ACCURACY; ANIMAL CELL; ANTIGEN DETECTION; ANTIGEN EXPRESSION; ARTICLE; AUTOMATION; CAPILLARY CYTOMETER; CELL MEMBRANE; CLINICAL PROTOCOL; CONTROLLED STUDY; HERPES SIMPLEX VIRUS 1; INCUBATION TEMPERATURE; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE; VIRUS PARTICLE; VIRUS TITRATION;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CELL LINE; CRICETINAE; FLOW CYTOMETRY; HERPESVIRUS 1, HUMAN; PLAQUE ASSAY; STATISTICS; STATISTICS AS TOPIC; VIRUSES;

HUMAN HERPESVIRUS 1; PSIDIUM GUAJAVA; REPLICATION COMPETENT VIRUSES;

EID: 33748683961     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.06.013     Document Type: Article

References (18)

1. Boekh M., and Boivin G. Quantitation of cytomegalovirus: methodological aspects and clinical applications. Clin. Microb. Rev. 11 3 (1998) 533-554
2. Bordignon J., Pires Ferreira S.C., Medeiros Caporale G.M., Carrieri M.L., Kotait I., Lima H.C., and Zanetti C.R. Flow cytometry assay for intracellular rabies virus detection. J. Virol. Meth. 105 (2002) 181-186
3. Buckland B.C. The process development challenge for a new vaccine. Nat. Med. 11 (2005) S16-S19
4. Elmendorf S., McSharry J., Laffin J., Fogleman D., and Lehman J.M. Detection of an early cytomegalovirus antigen with two-colour quantitative flow cytometry. Cytometry 9 (1998) 254-260
5. Gompels U.A., and Minson A.C. Antigenic properties and cellular localization of herpes simplex virus glycoprotein H synthesized in a mammalian cell expression system. J. Virol. 63 11 (1989) 4744-4755
6. Harland, J., Brown, S.M., 1998. HSV growth preparation and assay. In: Brown, S.M., Maclean, A.R. (Ed.), Methods in Molecular Medicine, vol. 10, Herpes Simplex Virus Protocols. Human Press Inc., Totowa, NJ.
7. Hi-Gung B., Nitsche A., Teichmann A., Biel S.S., and Niedrig M. Detection of yellow fever virus: a comparison of quantitative real-time PCR and plaque assay. J. Virol. Meth. 110 (2003) 185-191
8. Jennings S.R., Lippe P.A., Pauza K.J., Spear P.G., Pereira L., and Tevethia S.S. Kinetics of expression of herpes simplex virus type-1 specific glycoprotein species on the surfaces of infected murine, simian, and human cells: flow cytometric analysis. J. Virol. 61 1 (1987) 104-112
9. Liu B.L., Robinson M., Han Z.Q., Branston R.H., English C., Reay P., McGrath Y., Thomas S.K., Thornton M., Bullock P., Love C.A., and Coffin R.S. ICP34.5 deleted herpes simplex virus with enhanced oncolytic, immune stimulating, and anti-tumour properties. Gene Ther. 10 4 (2003) 292-303
10. Loftian P., Levy M.S., Coffin R.S., Fearn T., and Ayazi-Shamlou P. The impact of process conditions on the centrifugal recovery of a disabled herpes simplex virus. Biotechnol. Prog. 19 (2003) 209-215
11. McSharry J., Costantino R., McSharry M.B., Venezia R.A., and Lehman J.M. Rapid detection of herpes simplex virus in clinical samples by flow cytometry after amplification in tissue culture. J. Clin. Microbiol. 28 8 (1990) 1864-1866
12. Miller M., and Howell C.L. Rapid detection and identification of herpes simplex virus in cell culture by a direct immunoperoxidase staining procedure. J. Clin. Microbiol. 18 3 (1983) 550-553
13. Neumann, J., Eis-Hubinger, and A.M., Koch, N., 2003. Herpes simplex virus type-1 targets the MHC class II processing pathways for immune invasion. J. Immunol. 171, 3075-3083.
14. Roback J.D., Barclay S., and Hillyer D. Improved method for fluorescence cytometric immunohematology testing. Transfusion 40 (2004) 513-520
15. 2) in virion infectivity. J. Virol. 29 3 (1979) 1149-1158
16. Scmidt N.J., Dennis J., Devlin V., Gallo D., and Mills J. Comparison of immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens. J. Clin. Microbiol. 18 2 (1983) 445-448
17. Thomas C.E., Ehrhardt A., and Kay M.A. Progress and problems with the use of viral vectors for gene therapy. Nat. Rev.: Genet. 4 (2004) 346-358
18. Thomas S.K., Lilley C.E., Latchman D.S., and Coffin R.S. Equine herpesvirus 1 gene 12 can substitute for vmw65 in the growth of herpes simplex virus (HSV) type-1, allowing the generation of optimized cell lines for the propagation of HSV vectors with multiple immediate-early gene defects. J. Virol. 73 9 (1999) 7399-7409