Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus

Journal of Veterinary Science

Volumn 7, Issue 3, 2006, Pages 249-255

  Muleke, Charles I ^a   Ruofeng, Yan ^a   Lixin, Xu ^a   Yanming, Sun ^a   Xiangrui, Li ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cysteine protease activity; Haemonchus contortus; Recombinant HC58 protein; Synthetic peptide substrates

Indexed keywords

CAPRA HIRCUS; HAEMONCHUS CONTORTUS;

EID: 33748645776     PISSN: 1229845X     EISSN: 1229845X     Source Type: Journal    
DOI: 10.4142/jvs.2006.7.3.249     Document Type: Article

References (20)

1. Baig S, Damian RT, Peterson DS. A novel cathepsin B active site motif is shared by helminth blood feeders. Exp Parasitol 2002, 101, 83-89.
2. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72, 248-254.
3. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987, 162, 156-159.
4. Geldhof P, Claerebout E, Knox DP, Jagneessens J, Vercruysse J. Proteinases released in vitro by the parasitic stages of the bovine abomasal nematode Ostertagia ostertagi. Parasitology 2000, 121, 639-647.
5. Hartman D, Donald DR, Nikolaou S, Savin KW, Hasse D, Presidente PJA, Newton SE. Analysis of developmentally regulated genes of the parasite Haemonchus contortus. Int J Parasitol 2001, 31, 1236-1245.
6. Hotez PJ, Cerami A. Secretion of a proteolytic anticoagulant by Ancylostoma hookworms. J Exp Med 1983, 157, 1594-1603.
7. Jasmer DP, Roth J, Myler PJ. Cathepsin B-like cysteine proteases and Caenorhabditis elegans homologues dominate gene products expressed in adult Haemonchus contortus intestine. Mol Biochem Parasitol 2001, 116, 159-169.
8. Jasmer DP, Mitreva MD, McCarter JP. mRNA sequences for Haemonchus contortus intestinal cathepsin B-like cysteine proteases display an extreme in abundance and diversity compared with other adult mammalian parasitic nematodes. Mol Biochem Parasitol 2004, 137, 297-305.
9. Knox DP, Redmond DL, Jones DG. Characterization of proteinases in extracts of adult Haemonchus contortus, the ovine abomasal nematode. Parasitology 1993, 106, 395-404.
10. Lourenco EV, Pereira SR, Faca VM, Coelho-Castelo AAM, Mineo JR, Roque-Barreira MC, Greene LJ, Panunto-Castelo A. Toxoplasma gondii micronemal protein MIC1 is a lactose-binding lectin. Glycobiology 2001, 11, 541-547.
11. Newton SE, Meeusen EN. Progress and new technologies for developing vaccines against gastrointestinal nematode parasites of sheep. Parasite Immunol 2003, 25, 283-296.
12. Newlands GFJ, Skuce PJ, Knox DP, Smith WD. Cloning and expression of cystatin, a potent cysteine protease inhibitor from the gut of Haemonchus contortus. Parasitology 2001, 122, 371-378.
13. Pratt D, Armes LG, Hageman R, Reynolds V, Boisvenue RJ, Cox GN. Cloning and sequence comparisons of four distinct cysteine proteases expressed by Haemonchus contortus adult worms. Mol Biochem Parasitol 1992, 51, 209-218.
14. Sajid M, Mckerrow JH, Hansell E, Mathieu MA, Lucas KD, Hsieh I, Greenbaum D, Bogyo M, Salter JP, Lim KC, Franklin C, Kim JH, Caffrey CR. Functional expression and characterization of Schistosoma mansoni cathepsin B and its trans-activation by an endogenous asparaginyl endopeptidase. Mol Biochem Parasitol 2003, 131, 65-75.
15. Sajid M, Mckerrow JH. Cysteine proteases of parasitic organisms. Mol Biochem Parasitol 2002, 120, 1-21.
16. Shompole S, Jasmer DP. Cathepsin B-like cysteine proteases confer intestinal cysteine protease activity in Haemonchus contortus. J Biol Chem 2001, 276, 2928-2934.
17. Skuce PJ, Redmond DL, Liddell S, Stewart EM, Newlands GFJ, Smith WD, Knox DP. Molecular cloning and characterization of gut-derived cysteine proteinases associated with a host protective extract from Haemonchus contortus. Parasitology 1999, 119, 405-412.
18. Stear MJ, Bishop SC, Doligalska M, Duncan JL, Holmes PH, Irvine J, McCririe L, McKeller QA, Sinski E, Murray M. Regulation of egg production, worm burden, worm length and worm fecundity by host responses in sheep infected with Ostertagia circumcincta. Parasite Immunol 1995, 17, 643-652.
19. Tort J, Brindley PJ, Knox D, Wolfe KH, Dalton JP. Proteinases and associated genes of parasitic helminths. Adv Parasitol 1999, 43, 161-266.
20. Williamson AL, Brindley PJ, Knox PK, Hotez PJ, Loukas A. Digestive proteases of blood-feeding nematodes. Trends Parasitol 2003, 19, 417-423.