Development of a real-time, differential RT-PCR TaqMan® assay for lyssavirus genotypes 1, 5 and 6

Developments in Biologicals

Volumn 126, Issue , 2006, Pages 227-236

  Wakeley, P R ^a   Johnson, N ^a   McElhinney, L M ^a   Marston, D ^a   Sawyer, J ^a   Fooks, A R ^a  

^a VETERINARY LABORATORIES AGENCY   (United Kingdom)

Author keywords

Genotyping; Rabies virus; TaqMan PCR

Indexed keywords

ANIMAL; ANIMAL DISEASE; BAT; CONFERENCE PAPER; GENETICS; GENOTYPE; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; RABIES; RABIES VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIROLOGY;

ANIMALS; CHIROPTERA; GENOTYPE; HUMANS; LYSSAVIRUS; RABIES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 33748597088     PISSN: 14246074     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Conference Paper

References (9)

1. Heaton, PR, Johnstone P, McElhinney LM, Cowley R, O'Sullivan E, Whitby JE: Heminested PCR assay for the detection of six genotypes of rabies and rabies-related viruses. J Clin Microbiol 1997;35:2762-2766.
2. th edition, in Meslin FX, Kaplan MM, Koprowski H (eds): World Health Organization. Geneva, Switzerland, 1996, pp 88-93.
3. th edition, in Meslin FX, Kaplan MM, Koprowski H (eds): World Health Organization, Geneva, Switzerland, 1996, pp 96-104.
4. th edition, in Meslin FX, Kaplan MM, Koprowski H (eds): World Health Organization, Geneva, Switzerland, 1996, pp 80-87.
5. Fooks AR, McElhinney LM, Marston DA, Selden D, Joliffe TA, Wakeley PR, Johnson N, Brookes S: Identification of a European bat lyssavirus type 2 in Daubenton's bat found in Staines, Surrey, UK. Vet Rec 2004;155:434-435.
6. Livak KJ, Scmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001;25:402-408.
7. Fooks AR, Selden D, Brookes S, Johnson N, Marston DA, Joliffe TA, Wakeley PR, McElhinney LM: Identification of a European bat lyssavirus type 2 in Daubenton's bat found in Lanchashire. Vet Rec 2004;155:606-607.
8. Solomon T, Marston D, Mallewa M, Felton T, Shaw S, McElhinney LM, Das K, Mansfield K, Wainwright J, Kwong GN, Fooks AR: Paralytic rabies after a two week holiday in India. BMJ. 2005;331:501-503.
9. Black EM, Lowings JP, Smith J, Heaton PR, McElhinney LM: A rapid RT-PCR method to differentiate six established genotypes of rabies and rabies-related viruses using TaqMan® technology. J Virol Methods 2002;105:25-35.