Examination of stability of bone marrow blood RNA in the PAXgene tube

Laboratory Hematology

Volumn 12, Issue 3, 2006, Pages 143-147

  Yamamoto, Toshiharu ^a   Sekiyama, Akiko ^a   Sekiguchi, Hironobu ^a   Yoshida, Tsutomu ^a   Miyagi, Yohei ^a  

^a KANAGAWA CANCER CENTER   (Japan)

Author keywords

Degradation; EDTA tube; PAXgene tube; Quantitative RT PCR; RNA

Indexed keywords

BCR ABL PROTEIN; COMPLEMENTARY DNA; MESSENGER RNA; PORPHOBILINOGEN DEAMINASE; RIBOSOME RNA; RNA; RNA 18S; RNA 28S;

ARTICLE; BLOOD SAMPLING; BONE MARROW EXAMINATION; CLINICAL ARTICLE; CONTROLLED STUDY; ELECTROPHORESIS; FUSION GENE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; LEUKEMIA REMISSION; MINIMAL RESIDUAL DISEASE; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; RNA EXTRACTION; RNA ISOLATION; RNA STABILITY; TUMOR SUPPRESSOR GENE;

BLOOD SPECIMEN COLLECTION; BONE MARROW CELLS; BONE MARROW EXAMINATION; HUMANS; LEUKEMIA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA STABILITY; RNA, MESSENGER; RNA, NEOPLASM;

EID: 33748582640     PISSN: 10802924     EISSN: None     Source Type: Journal    
DOI: 10.1532/LH96.05021     Document Type: Article

References (9)

1. van der Velden VH, Hochhaus A, Cazzaniga G, Szczepanski T, Gabert J, van Dongen JJ. Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects. Leukemia. 2003;17:1013-1034.
2. Rainen L, Oelmueller U, Jungensen S, et al. Stabilization of mRNA expression in whole blood samples. Clin Chem. 2002;48:1883-1890.
3. Muller MC, Merx K, Weisser A, et al. Improvement of molecular monitoring of residual disease in leukemias by beside RNA stabilization. Leukemia. 2002;16:2395-2399.
4. Thach DC, Lin B, Walter E, et al. Assessment of two methods for handling blood in collection tubes with RNA stabilizing agent for surveillance of gene expression profiles with high density microarrays. J Immunol Methods. 2003;3238:269-279.
5. Stordeur P, Zhou L, Byl B, et al. Immune monitoring in whole blood using real-time PCR. J Immuno Methods. 2003;276:69-77.
6. Tobal K, Newton J, Macheta M, et al. Molecular quantitation of minimal residual disease in acute myeloid leukemia with t(8;21) can identify patients in durable remission and predict clinical relapse. Blood. 2000;95:815-819.
7. Tobal K, Moore H, Macheta M, Yin JA. Monitoring minimal residual disease and predicting relapse in APL by quantitating PML-RARalpha transcripts with a sensitive competitive RT-PCR method. Leukemia. 2001;15:1060-1065.
8. Miller CL, Diglisic S, Leister F, Webster M, Yolken RH. Evaluating RNA status for RT-PCR in extracts of postmortem human brain tissue. Biotechniques. 2004;36:628-633.
9. Auer H, Lyianarachchi S, Newsom D, Klisovic MI, Marcucci G, Kornacker K. Chipping away at the chip bias: RNA degradation in microarray analysis. Nat Genet. 2003;35:292-293.