Antimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells

Biochimica et Biophysica Acta - General Subjects

Volumn 1760, Issue 9, 2006, Pages 1323-1332

  Diz, Mariângela S S ^a   Carvalho, André O ^a   Rodrigues, Rosana ^a   Neves Ferreira, Ana Gisele C ^b   Da Cunha, Maura ^c   Alves, Elias Walter ^a   Okorokova Façanha, Anna L ^a   Oliveira, Marco Antônio ^a,c   Perales, Jonas ^b   Machado, Olga L T ^a   Gomes, Valdirene M ^a  

^a UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

^c INSTITUTO DE PESQUISA E DESENVOLVIMENTO   (Brazil)

Author keywords

Candida albicans; Capsicum annuum; Lipid transfer protein; Membrane permeabilization; Plant antimicrobial peptide; Saccharomyces cerevisiae

Indexed keywords

2 DIETHYLAMINOETHANOL; CYSTEINE DERIVATIVE; DYE; LIPID TRANSFER PROTEIN; PHOSPHATE; POLYPEPTIDE ANTIBIOTIC AGENT; SEPHAROSE; SYTOX GREEN; UNCLASSIFIED DRUG; ACID; ANTIFUNGAL AGENT; GLUCOSE; PEPTIDE; VEGETABLE PROTEIN;

ACIDIFICATION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CANDIDA ALBICANS; CELL MEMBRANE PERMEABILITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CULTURE MEDIUM; EXTRACTION; FUNGICIDAL ACTIVITY; FUNGUS HYPHAE; ION EXCHANGE CHROMATOGRAPHY; MORPHOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPPER; PH; PLANT SEED; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SACCHAROMYCES CEREVISIAE; SCANNING ELECTRON MICROSCOPY; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY; YEAST CELL; BIOSYNTHESIS; CELL PROLIFERATION; CHEMISTRY; CYTOLOGY; DRUG EFFECT; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; YEAST;

CANDIDA ALBICANS; CAPSICUM ANNUUM; CAPSICUM ANNUUM VAR. ANNUUM; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

ACIDS; AMINO ACID SEQUENCE; ANTIFUNGAL AGENTS; CAPSICUM; CELL MEMBRANE PERMEABILITY; CELL PROLIFERATION; CULTURE MEDIA; GLUCOSE; HYDROGEN-ION CONCENTRATION; MICROSCOPY, ELECTRON, SCANNING; MOLECULAR SEQUENCE DATA; PEPTIDES; PLANT PROTEINS; SEEDS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; YEASTS;

EID: 33748540703     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbagen.2006.04.010     Document Type: Article

References (39)

1. Broekaert W.F., Cammue B.P.A., De Bolle M.F.C., Thevissen K., De Samblanx G., and Osborn R.W. Antimicrobial peptides from plants. Crit. Rev. Plant Sci. 16 (1997) 297-323
2. Cammue B.P.A., De Bolle M.G.C., Terras F.R.G., Proost P., Van Damme J., Rees S.B., Vanderleyden J., and Broekaert W.F. Isolation and characterization of a novel class of plant antimicrobial peptides from Mirabilis jalapa L. seeds. J. Biol. Chem. 267 (1992) 2228-2233
3. Broekaert W.F., Marrien W., Terras F.R.G., De Bolle M.F.C., Proost P., Van Damme J., Dillen L., Claeys M., Ress S.B., Vanderleyden J., and Cammue B.P.A. Antimicrobial peptides from Amaranthus caudatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin binding proteins. Biochemistry 31 (1992) 4308-4314
4. Duvick J.P., Rood T., Rao A.G., and Marshak D.R. Purification and characterization of a novel antimicrobial peptide from maize (Zea mays L.) kernels. J. Biol. Chem. 267 (1992) 18814-18820
5. Garcia-Olmedo F., Rodriguez-Palenzuela P., Hernandez-Lucas C., Ponz F., Maraña C., Carmona M.J., Lopez-Fando J.J., Fernandez J.A., and Carbonero P. The thionins: a protein family that includes purothionins, viscotoxins and cambrins. Plant Mol. Cell Biol. 6 (1989) 31-60
6. Cammue B.P.A., Thevissen K., Hendriks M., Eggermont K., Goderis I.J., Proost P., Van Damme J., Osborn R.W., Guerbette F., Kader J.-C., and Broekaert W.F. A potent antimicrobial protein from onion (Allium cepa L.) seeds showing sequence homology to plant lipid transfer proteins. Plant Physiol. 109 (1995) 445-455
7. Terras F.R.G., Schoofs H.M.E., De Bolle M.F.C., Van Leuven F., Ress S.B., Vanderleyden J., Cammue B.P.A., and Broekaert W.F. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 267 (1992) 15301-15309
8. Terras F.R.G., Eggermont K., Kovaleva V., Raikhel N.V., Osborn R.W., Kester A., Rees S.B., Torrekens S., Van Leuven F., Vanderleyden J., Cammue B.P.A., and Broekaert W.F. Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7 (1995) 573-588
9. Broekaert W.F., Terras F.R.G., Cammue B.P.A., and Osborn R.W. Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol. 108 (1995) 1353-1358
10. Kader J.-C. Lipid-transfer protein in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 (1996) 627-654
11. Tsuboi S., Osafune T., Tsugeki R., Nishimura M., and Yamada M. Nonspecific lipid transfer protein in castor bean cotyledons cells: subcellular localization and a possible role in lipid metabolism. J. Biochem. 111 (1992) 500-508
12. Pyee J., Yu H., and Kolattukudy P.E. Identification of a lipid transfer protein as the major protein in the surface wax of broccoli (Brassica oleracea) leaves. Archives Biochem. Biophys. 311 (1994) 460-468
13. Carvalho A.O., Machado O.L.T., Cunha M., Santos I.S., and Gomes V.M. Antimicrobial peptides and immunolocalization of a LTP in Vigna unguiculata seeds. Plant Physiol. Biochem. 39 (2001) 137-146
14. Carvalho A.O., Teodoro C.E.S., Da Cunha M., Okorokova-Façanha A.L., Okorokov L.A., Fernandes K.V.S., and Gomes V.M. Intracellular localization of a lipid transfer protein in Vigna unguiculata seeds. Physiol. Plant. 122 (2004) 328-336
15. Terras F.R.G., Schoofs H.M.E., De Bolle M.F.C., Van Leuven F., Ress S.B., Vanderleyden J., Cammue B.P.A., and Broekaert W.F. Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds. J. Biol. Chem. 267 (1992) 15301-15309
16. Molina A., Segura A., and García-Olmedo F. Lipid transfer protein (ns LTP) from barley and maize leaves are potent inhibitors of bacterial and fungal pathogens. FEBS 316 (1993) 119-122
17. Segura A., Moreno M., and García-Olmedo F. Purification and antipathogenic activity of lipid transfer protein (LTPs) from the leaves of Arabidopsis and spinach. FEBS 332 (1993) 243-246
18. 1) of Triticum aestivum seeds. Relationships with their in vitro antifungal properties. Plant Sci. 138 (1998) 121-135
19. Gonorazky A.G., Regente M.C., and de la Canal L. Stress induction and antimicrobial properties of a lipid transfer protein in germinating sunflower seeds. J. Plant Physiol. 162 (2005) 618-624
20. Velazhahan R., Radhajeyalakshmi R., Thangavelu R., and Muthukrishnan S. An antifungal protein purified from pearl millet seeds shows sequence homology to lipid transfer proteins. Biol. Plant. 44 (2001) 417-421
21. Gomes V.M., Okorokov L.A., Rose T.L., Fernandes K.V.S., and Xavier-Filho J. Legume Vicilins (7S storage globulin) and glucose stimulated acidification of medium by yeast cell. Biochim. Biophys. Acta 1379 (1998) 207-216
22. Riva E.M., Rodrigues R., Pereira M.G., Sudré C.P., Karasawa M., and Amaral Jr. A.T. Inheritance of bacterial spot disease in Capsicum annuum L. Crop Breed. Appl. Biotechnol. 4 (2004) 490-494
23. Schagger H., and Von Jagow G. Tricine-sodium dodecylsulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166 (1987) 368-379
24. Towbin H., Staehelin T.E., and Gordon J. Eletrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U. S. A. 76 (1979) 4350-4354
25. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215 (1990) 403-410
26. Broekaert W.F., Terras F.R.G., Cammue B.P.A., and Vanderleyden J. An automated quantitative assay for fungal growth inhibition. FEMS Microbiol. Lett. 69 (1990) 55-60
27. Thevissen K., Terras F.R.G., and Broekaert W.F. Permeabilization of fungal membranes by plant defensins inhibits fungal growth. Appl. Envirom. Microbiol. 65 (1999) 5451-5458
28. Do H.M., Lee S.C., Jung H.W., Sohn K.H., and Hwang B.K. Differential expression and in situ localization of a pepper defensin (CADEF1) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsicum annuum. Plant Sci. 166 (2004) 1297-1305
29. Park C.J., Shin R., Park J.M., Lee G.J., Youl J.S., and Paek K.H. Induction of pepper cDNA enconding a lipid transfer protein during the resistance response to tobacco mosaic virus. Plant Mol. Biol. 48 (2002) 243-254
30. Oh B.J., Ko M.K., Kostenyuk I., Shin B., and Kim K.S. Coexpression of a defensin gene and thionin-like gene via different signal transduction pathways in pepper and colletotrichum gloesporioides interactions. Plant Mol. Biol. 41 (1999) 313-319
31. Lee Y.M., Wee H.S., Ahn I.P., Lee Y.H., and An C.S. Molecular Characterization of a cDNA for a cysteine-rich antifungal protein from Capsicum annuum. J. Plant Biol. 47 (2004) 375-382
32. Kader J.C. Lipid-transfer proteins: a puzzling family of plant proteins. Trends Plant Sci. 2 (1997) 66-70
33. Osborn R.W., De Samblanx G.W., Thevissen K., Goderis I., Torrenkens S., Van Leuven F., Attenborough S., Ress S.B., and Broekaert W.F. Isolation and characterization of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 368 (1995) 257-262
34. Sitaram N., and Nagaraj R. Interaction of antimicrobial peptides with biological and model membranes: structural and charge requirements for activity. Biochim. Biophys. Acta, Biomembr. 1462 (1999) 29-54
35. Thevissen K., Osborn R.W., Acland D.P., and Broekaert W.F. Specific, high affinity binding sites from an antifungal plant defensin on Neurospora crassa hyphae and microsomal membranes. J. Biol. Chem. 272 (1997) 32176-32181
36. Terras F.R.G., Ergermont K., Kovaleva V., Raikhel N.V., Osbourn R.W., Kester A., Rees S.B., Torrekens S., Van Leuden F., Vanderleyden J., Cammue B.P.A., and Broekaert W.F. Small cystein-rich antifungal proteins from radish: their role in host defense. Plant Cell 7 (1995) 573-588
37. Gallo R.L., Murakami M., Ohtake T., and Zaiou M. Biology and clinical relevance of naturally occurring antimicrobial peptides. J. Allergy Clin. Immunol. 110 (2002) 823-831
38. Soll D.R. Candida albicans. In: Szaniszlo P.J. (Ed). Fungal dimorphism with emphasis on fungi pathogenic for humans (1985), Plenum, New York 167-195
39. + transport, salt tolerance and morphogenesis. Microbiology 146 (2000) 1035-1044