Sampling strategy for intraoral detection of periodontal pathogens before and following periodontal therapy

Journal of Periodontology

Volumn 77, Issue 8, 2006, Pages 1323-1332

  Beikler, Thomas ^a   Schnitzer, Stefan ^b   Abdeen, Ghiath ^b   Ehmke, Benjamin ^b   Eisenacher, Martin ^b   Flemmig, Tmomas F ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Analysis; Bacteria; Microbiology; Periodontal diseases microbiology; Testing

Indexed keywords

BACTERIAL DNA;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ADULT; AGED; ARTICLE; BACTERIAL COLONIZATION; BACTERIUM CARRIER; BACTERIUM DETECTION; CHEEK MUCOSA; CHRONIC DISEASE; CLINICAL ARTICLE; CONTROLLED STUDY; EIKENELLA CORRODENS; FEMALE; GINGIVA; HUMAN; MALE; MICROBIOLOGICAL EXAMINATION; MOUTH FLORA; PERIODONTITIS; POLYMERASE CHAIN REACTION; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; PREVOTELLA NIGRESCENS; RELIABILITY; SALIVA ANALYSIS; TANNERELLA FORSYTHENSIS; THROAT CULTURE; TONGUE; TONSIL; TOOTH PLAQUE; TREPONEMA DENTICOLA; ANAEROBIC BACTERIUM; BACTERIAL COUNT; HETEROZYGOTE; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; MOUTH MUCOSA; PROBABILITY; SALIVA;

BACTERIA, ANAEROBIC; CARRIER STATE; CHRONIC DISEASE; COLONY COUNT, MICROBIAL; DENTAL PLAQUE; DNA, BACTERIAL; FEMALE; HUMANS; MALE; MIDDLE AGED; MOUTH MUCOSA; PERIODONTITIS; POLYMERASE CHAIN REACTION; PROBABILITY; SALIVA;

EID: 33748522747     PISSN: 00223492     EISSN: None     Source Type: Journal    
DOI: 10.1902/jop.2006.050204     Document Type: Article

References (31)

1. Wolff LF, Aeppli DM, Pihlström B, et al. Natural distribution of 5 bacteria associated with periodontal disease. J Clin Periodontol 1993;20:699-706.
2. Ali RW, Velcescu C, Jivanescu MC, Lofthus B, Skaug N. Prevalence of 6 putative periodontal pathogens in subgingival plaque samples from Romanian adult periodontitis patients. J Clin Periodontol 1996;23: 133-139.
3. van Winkelhoff AJ, Loos BG, van der Reijden WA, van der Velden Ü. Porphyromonas gingivalis. Bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction. J Clin Periodontol 2002;29:1023-1028.
4. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25:134-144.
5. Beikler T, Prior K, Ehmke B, Flemmig TF. Specific antibiotics in the treatment of periodontitis - a proposed strategy. J Periodontol 2004;75:169-175.
6. Beikler T, Abdeen G, Schnitzer S, et al. Microbiological shifts in intra- and extraoral habitats following mechanical periodontal therapy. J Clin Periodontol 2004; 31:777-783.
7. Quirynen M, De Soete M, Dierickx K, van Steenberghe D. The intraoral translocation of periodontopathogens jeopardises the outcome of periodontal therapy. A review of the literature. J Clin Periodontol 2001;28: 499-507.
8. Bollen CM, Mongardini C, Papaioannou W, van Steenberghe D, Quirynen M. The effect of a one-stage full-mouth disinfection on different intra-oral niches. Clinical and microbiological observations. J Clin Periodontol 1998;25:56-66.
9. Koshy G, Corbet EF, Ishikawa I. A full-mouth disinfection approach to nonsurgical periodontal therapy - Prevention of reinfection from bacterial reservoirs. Periodontol 2000 2004;36:166-178.
10. Quirynen M, Mongardini C, Pauwels M, Bollen CM, Van Eldere J, van Steenberghe D. One stage full- versus partial-mouth disinfection in the treatment of chronic adult or generalized early-onset periodontitis. II. Longterm impact on microbial load. J Periodontol 1999; 70:646-656.
11. Slots J, Jorgensen MG. Effective, safe, practical and affordable periodontal antimicrobial therapy: Where are we going, and are we there yet? Periodontol 2000 2002;28:298-312.
12. American Academy of Periodontology. Systemic antibiotics in periodontics (position paper). J Periodontol 1996;67:831-838.
13. Tonjum T, Haas R. Identification of Actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays. J Clin Microbiol 1993;31:1856-1859.
14. Bodinka A, Schmidt H, Henkel B, Flemmig TF, Klaiber B, Karch H. Polymerase chain reaction for the identification of Porphyromonas gingivalis collagenase genes. Oral Microbiol Immunol 1994;9:161-165.
15. Slots J, Ashimoto A, Flynn MJ, Li G, Chen C. Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction. Clin Infect Dis 1995; 20(Suppl. 2):S304-S307.
16. Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol 1996;11:266-273.
17. Kwok S, Higuchi R. Avoiding false positives with PCR. Nature 1989;339:237-238.
18. Takamatsu N, Yano K, He T, Umeda M, Ishikawa I. Effect of initial periodontal therapy on the frequency of detecting Bacteroides forsythus, Porphyromonas gingivalis, and Actinobacillus actinomycetemcomitans. J Periodontol 1999;70:574-580.
19. Mombelli A, McNabb H, Lang NP. Black-pigmenting Gram-negative bacteria in periodontal disease. II. Screening strategies for detection of P. gingivalis. J Periodontal Res 1991;26:308-313.
20. Mantilla Gomez S, Danser MM, Sipos PM, Rowshani B, van der Velden U, van der Weijden GA. Tongue coating and salivary bacterial counts in healthy/ gingivitis subjects and periodontitis patients. J Clin Periodontol 2001;28:970-978.
21. Rams TE, Babalola OO, Slots J. Subgingival occurrence of enteric rods, yeasts and staphylococci after systemic doxycycline therapy. Oral Microbiol Immunol 1990;5:166-168.
22. Pavicic MJ, van Winkelhoff AJ, Douque NH, Steures RW, de Graaff J. Microbiological and clinical effects of metronidazole and amoxicillin in Actinobacillus actinomycetemcomitans-associated periodontitis. A 2-year evaluation. J Clin Periodontol 1994; 21:107-112.
23. van Winkelhoff AJ, Tijhof CJ, de Graaff J. Microbiological and clinical results of metronidazole plus amoxicillin therapy in Actinobacillus actinomycetemcomitans-associated periodontitis. J Periodontol 1992; 63:52-57.
24. Shiloah J, Patters MR, Dean JW III, Bland P, Toledo G. The prevalence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Bacteroides forsythus in humans 1 year after 4 randomized treatment modalities. J Periodontol 1998;69:1364-1372.
25. Ximenez-Fyvie LA, Haffajee AD, Socransky SS. Microbial composition of supra- and subgingival plaque in subjects with adult periodontitis. J Clin Periodontol 2000;27:722-732.
26. Eger T, Zoller L, Muller HP, Hoffmann S, Lobinsky D. Potential diagnostic value of sampling oral mucosal surfaces for Actinobacillus actinomycetemcomitans in young adults. Eur J Oral Sci 1996;104:112-117.
27. Umeda M, Contreras A, Chen C, Bakker I, Slots J. The utility of whole saliva to detect the oral presence of periodontopathic bacteria. J Periodontol 1998;69: 828-833.
28. Muller HP, Eickholz P, Heinecke A, Pohl S, Muller RF, Lange DE. Simultaneous isolation of Actinobacillus actinomycetemcomitans from subgingival and extra-crevicular locations of the mouth. J Clin Periodontol 1995;22:413-419.
29. Christersson LA, Fransson CL, Dunford RG, Zambon JJ. Subgingival distribution of periodontal pathogenic microorganisms in adult periodontitis. J Periodontol 1992;63:418-425.
30. Haffajee AD, Socransky SS. Effect of sampling strategy on the false-negative rate for detection of selected subgingival species. Oral Microbiol Immunol 1992;7: 57-59.
31. Gunsolley JC, Chinchilli VN, Savitt ED, et al. Analysis of site specific periodontal bacteria sampling schemes. J Periodontol 1992;63:507-514.