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15N distances by this treatment as a three-spin system were found to be 3.46 and 4.12 Å, respectively. These data are obviously within the claimed level of error as ±0.05 Å evaluated by the present two-spin system.
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85087582863
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note
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27,31,32 This is not the case, however, when the two peaks crossed over at certain Ψ angles.
-
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44
-
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33748500901
-
-
note
-
This does not necessarily mean that the accurate REDOR parameters are available with a longer π pulse such as 50 μs even if the condition of 10% of the rotor cycle is satisfied. This is because utilization of a longer π pulse may cause an additional error caused by insufficient excitation range for the entire spin system.
-
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-
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45
-
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33748513341
-
-
note
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2 value for the carbonyl carbon was 27.4 ms under the present spectrometer condition.
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46
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0028804827
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33748482052
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36
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54
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33748492829
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note
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2).
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33748514244
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The broadened shoulder peak was unequivocally assigned to the Cγ carbon in view of the present X-ray diffraction and MD simulation data
-
The broadened shoulder peak was unequivocally assigned to the Cγ carbon in view of the present X-ray diffraction and MD simulation data.
-
-
-
-
58
-
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33748498538
-
-
note
-
43 Therefore, the force field used here is suitable for determination of the conformations of the peptide, which is very flexible.
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