The effect of the single substitution of arginine within the RGD tripeptide motif of a modified neurotoxin dendroaspin on its activity of platelet aggregation and cell adhesion

Cell Communication and Adhesion

Volumn 13, Issue 3, 2006, Pages 171-183

  Lu, Xinjie ^a   Davies, Joanna ^a   Lu, Dong ^a   Xia, Min ^a   Wattam, Beth ^a   Shang, Dazhuang ^a   Sun, Yingqing ^a,b   Scully, Mike ^a   Kakkar, Vijay ^a  

^a THROMBOSIS RESEARCH INSTITUTE   (United Kingdom)

^b BURNHAM INSTITUTE   (United States)

Author keywords

5 1; IIb 3; V 3; Dendroaspin; Integrin

Indexed keywords

ADENOSINE DIPHOSPHATE; ALANINE; ARGININE; ARGINYLGLYCYLASPARTIC ACID; BETA1 INTEGRIN; BETA3 INTEGRIN; DENDROASPIN; FIBRINOGEN; FIBRONECTIN; GLUTAMINE; HISTIDINE; INTEGRIN; LYSINE; MUTANT PROTEIN; NEUROTOXIN; UNCLASSIFIED DRUG; VERY LATE ACTIVATION ANTIGEN 5; VITRONECTIN RECEPTOR;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CELL ADHESION; CELL ASSAY; CONTROLLED STUDY; DRUG TARGETING; ERYTHROLEUKEMIA; HUMAN; HUMAN CELL; MELANOMA CELL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN MOTIF; THROMBOCYTE AGGREGATION INHIBITION;

ADENOSINE DIPHOSPHATE; AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; ANIMALS; ARGININE; CELL ADHESION; CELL LINE, TUMOR; ELAPID VENOMS; ELAPIDAE; FIBRINOGEN; FIBRONECTINS; HUMANS; MOLECULAR WEIGHT; MUTANT PROTEINS; MUTATION; NEUROTOXINS; OLIGOPEPTIDES; PLATELET AGGREGATION;

DENDROASPIS;

EID: 33748465915     PISSN: 15419061     EISSN: 15435180     Source Type: Journal    
DOI: 10.1080/15419060600726183     Document Type: Article

References (37)

1. Ruoslahti, E. (1996) RGD and other recognition sequences for integrins Annu Rev Cell Dev Biol, 12, pp. 697-715. http://www4.infotrieve.com/newmedline/ detail.asp?NameID=8970741&Session=&searchQuery=Annu+Rev+Cell+Dev+ Biol%5BJournal+Name%5D+AND+12%5BVolume%5D+AND+697%5BPage+Number%5D+AND+ 1996%5BPublication+Date%5D+AND+Ruoslahti%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=1081-0706&vol=12&iss= &firstpage=697 http://dx.doi.org/10.1146%2Fannurev.cellbio.12.1.697
2. Marcinkiewicz, C. (2005) Functional characteristic of snake venom disintegrins: Potential therapeutic implication Curr Pharm Des, 11, pp. 815-827. http://www4.infotrieve.com/newmedline/detail.asp?NameID= 15777236&Session=&searchQuery=Curr+Pharm+Des%5BJournal+Name%5D+ AND+11%5BVolume%5D+AND+815%5BPage+Number%5D+AND+2005%5BPublication+ Date%5D+AND+Marcinkiewicz%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=1381-6128&vol= 11&iss=&firstpage=815 http://dx.doi.org/10.2174%2F1381612053381765
3. Lu, X and Deadman, J and Williams, J and Kakkar, V and Rahman, S. (1993) Synthetic RGD peptides derived from the adhesive domains of snake-venom proteins: Evaluation as inhibitors of platelet aggregation Biochem J, 296, pp. 21-24. http://www4.infotrieve.com/newmedline/ detail.asp?NameID=8250845&Session=&searchQuery=Biochem+J% 5BJournal+Name%5D+AND+296%5BVolume%5D+AND+21%5BPage+Number%5D+AND+ 1993%5BPublication+Date%5D+AND+Lu%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0264-6021&vol= 296&iss=&firstpage=21
4. Scarborough, R and Rose, J and Hsu, M and Phillips, D and Fried, V and Campbell, A and Nannizzi, L and Charo, I and Barbourin, A. (1991) GPIIb-IIIa-specific integrin antagonist from the venom of Sistrurus m. barbouri J Biol Chem, 266, pp. 9359-9362. http://www4.infotrieve.com/ newmedline/detail.asp?NameID=2033037&Session=&searchQuery= J+Biol+Chem%5BJournal+Name%5D+AND+266%5BVolume%5D+AND+9359%5BPage+ Number%5D+AND+1991%5BPublication+Date%5D+AND+Scarborough%5BAuthor+ Name%5D&count=1 http://www.csa.com/htbin/
5. Takagi, J. (2004) Structural basis for ligand recognition by RGD (Arg-Gly-Asp)-dependent integrins Biochem Soc Trans, 32, pp. 403-406. http://www4.infotrieve.com/newmedline/detail.asp?NameID=15157147& Session=&searchQuery=Biochem+Soc+Trans%5BJournal+Name%5D+AND+32% 5BVolume%5D+AND+403%5BPage+Number%5D+AND+2004%5BPublication+Date%5D+ AND+Takagi%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0300-5127&vol= 32&iss=&firstpage=403 http://dx.doi.org/10.1042%2FBST0320403
6. Sanger, F and Nicklen, S and Coulson, A. (1977) DNA sequencing with chain-terminating inhibitors Proc Natl Acad Sci USA, 74, pp. 5463-5467. http://www4.infotrieve.com/newmedline/detail.asp?NameID=271968& Session=&searchQuery=Proc+Natl+Acad+Sci+USA%5BJournal+Name%5D+ AND+74%5BVolume%5D+AND+5463%5BPage+Number%5D+AND+1977%5BPublication+ Date%5D+AND+Sanger%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0027-8424&vol=74&iss= &firstpage=5463 http://dx.doi.org/10.1073%2Fpnas.74.12.5463
7. Lu, X and Lu, D and Scully, M and Kakkar, V. (2003) Modulation of integrin-binding selectivity by mutation within the RGD-loop of snake venom proteins: A novel drug development approach Curr Med Chem Cardiovasc Hematol Agents, 1, pp. 189-196. http://www4.infotrieve.com/newmedline/detail.asp?NameID=15320698& Session=&searchQuery=Curr+Med+Chem+Cardiovasc+Hematol+Agents% 5BJournal+Name%5D+AND+1%5BVolume%5D+AND+189%5BPage+Number%5D+AND+ 2003%5BPublication+Date%5D+AND+Lu%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=&vol=1&iss= &firstpage=189 http://dx.doi.org/10.2174%2F1568016033477522
8. Eto, K and Huet, C and Tarui, T and Kupriyanov, S and Liu, H and Puzon-McLaughlin, W and Zhang, X and Sheppard, D and Engvall, E and Takada, Y. (2002) Functional classification of ADAMs based on a conserved motif for binding to integrin alpha 9 beta 1: Implications for sperm-egg binding and other cell interactions J Biol Chem, 277, pp. 17804-17810. http://www4.infotrieve.com/newmedline/detail.asp?NameID= 11882657&Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+ AND+277%5BVolume%5D+AND+17804%5BPage+Number%5D+AND+2002%5BPublication+ Date%5D+AND+Eto%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0884-3996&vol= 277&iss=&firstpage=17804 http://dx.doi.org/10.1074%2Fjbc.M200086200
9. Arnaout, M. (2002) Integrin structure: New twists and turns in dynamic cell adhesion Immunol Rev, 186, pp. 25-40. http://www.csa.com/ htbin/linkabst.cgi?issn=0105-2896&vol=186&iss=&firstpage=25 http://dx.doi.org/10.1034%2Fj.1600-065X.2002.18612.x
10. Seow, K and Xiong, J and Arnaout, M and Welge, J and Rippmann, F and Goodman, S. (2002) Divalent cations and the relationship between alpha A and beta A domains in integrins Biochem Pharmacol, 64, pp. 805-812. http://www4.infotrieve.com/newmedline/detail.asp?NameID=12213573& Session=&searchQuery=Biochem+Pharmacol%5BJournal+Name%5D+AND+ 64%5BVolume%5D+AND+805%5BPage+Number%5D+AND+2002%5BPublication+Date%5D+ AND+Seow%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/ linkabst.cgi?issn=0006-2952&vol=64&iss=&firstpage=805 http://dx.doi.org/10.1016%2FS0006-2952%2802%2901142-5
11. Williams, J and Lu, X and Rahman, S and Keating, C and Kakkar, V. (1992) Dendroaspin: A potent integrin receptor inhibitor from the venoms of Dendroaspis viridis and D. jamesonii Biochem Soc Trans, 21, pp. 73S. http://www.csa.com/htbin/linkabst.cgi?issn=0300-5127&vol=21&iss= &firstpage=73S
12. Lu, X and Sun, Y and Shang, D and Wattam, B and Egglezou, S and Hughes, T and Hyde, E and Scully, M and Kakkar, V. (2001) Evaluation of the role of proline residues flanking the RGD motif of dendroaspin, an inhibitor of platelet aggregation and cell adhesion Biochem J, 355, pp. 633-638. http://www4.infotrieve.com/newmedline/detail.asp?NameID=11311124& Session=&searchQuery=Biochem+J%5BJournal+Name%5D+AND+355% 5BVolume%5D+AND+633%5BPage+Number%5D+AND+2001%5BPublication+Date%5D+ AND+Lu%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/ linkabst.cgi?issn=0264-6021&vol=355&iss=&firstpage=633
13. Lu, X and Rahman, S and Kakkar, V and Authi, K. (1996) Substitutions of proline 42 to alanine and methionine 46 to asparagine around the RGD domain of the neurotoxin dendroaspin alter its preferential antagonism to that resembling the disintegrin elegantin J Biol Chem, 271, pp. 289-294. http://www4.infotrieve.com/newmedline/detail.asp?NameID= 8550575&Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+ AND+271%5BVolume%5D+AND+289%5BPage+Number%5D+AND+1996%5BPublication+ Date%5D+AND+Lu%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/ linkabst.cgi?issn=0884-3996&vol=271&iss=&firstpage=289 http://dx.doi.org/10.1074%2Fjbc.271.1.289
14. Sambrook, J and Fritsch, E and Maniatis, T.(1989) Molecular cloning: A laboratory manual, 2nd Ed. Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press.
15. Ruggeri, Z. (2002) Platelets in atherothrombosis Nature Med., 8, pp. 1227-1234. http://www4.infotrieve.com/newmedline/detail.asp?NameID= 12411949&Session=&searchQuery=Nature+Med%2E%5BJournal+Name% 5D+AND+8%5BVolume%5D+AND+1227%5BPage+Number%5D+AND+2002%5BPublication+ Date%5D+AND+Ruggeri%5BAuthor+Name%5D&count=1 http://www.csa.com/ htbin/linkabst.cgi?issn=1078-8956&vol=8&iss=&firstpage=1227 http://dx.doi.org/10.1038%2Fnm1102-1227
16. Sudhakar, A and Sugimoto, H and Yang, C and Lively, J and Zeisberg, M and Kalluri, R. (2003) Human tumstatin and human endostatin exhibit distinct antiangiogenic activities mediated by alpha v beta 3 and alpha 5 beta 1 integrins Proc Natl Acad Sci USA, 100, pp. 4766-4771. http://www4.infotrieve.com/newmedline/detail.asp?NameID=12682293& Session=&searchQuery=Proc+Natl+Acad+Sci+USA%5BJournal+Name%5D+AND+ 100%5BVolume%5D+AND+4766%5BPage+Number%5D+AND+2003%5BPublication+ Date%5D+AND+Sudhakar%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0027-8424&vol=100& iss=&firstpage=4766 http://dx.doi.org/10.1073%2Fpnas.0730882100
17. Xiao, T and Takagi, J and Coller, B and Wang, J and Springer, T. (2004) Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics Nature, 432, pp. 59-67. http://www4.infotrieve.com/newmedline/detail.asp?NameID=15378069& Session=&searchQuery=Nature%5BJournal+Name%5D+AND+432%5BVolume% 5D+AND+59%5BPage+Number%5D+AND+2004%5BPublication+Date%5D+AND+Xiao% 5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/ linkabst.cgi?issn=0028-0887&vol=432&iss=&firstpage=59 http://dx.doi.org/10.1038%2Fnature02976
18. Xiong, J and Stehle, T and Zhang, R and Joachimiak, A and Frech, M and Goodman, S and Arnaout, M. (2002) Crystal structure of the extracellular segment of integrin alpha V beta 3 in complex with an Arg-Gly-Asp ligand Science, 296, pp. 151-155. http://www4.infotrieve.com/newmedline/ detail.asp?NameID=11884718&Session=&searchQuery=Science% 5BJournal+Name%5D+AND+296%5BVolume%5D+AND+151%5BPage+Number%5D+AND+ 2002%5BPublication+Date%5D+AND+Xiong%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0294-0337&vol=296& iss=&firstpage=151 http://dx.doi.org/10.1126%2Fscience.1069040
19. Cluzel, C and Saltel, F and Lussi, J and Paulhe, F and Imhof, B and Wehrle-Haller, B. (2005) The mechanisms and dynamics of {alpha}v{beta}3 integrin clustering in living cells J Cell Biol, 171, pp. 383-392. http://www4.infotrieve.com/newmedline/detail.asp?NameID=16247034& Session=&searchQuery=J+Cell+Biol%5BJournal+Name%5D+AND+171% 5BVolume%5D+AND+383%5BPage+Number%5D+AND+2005%5BPublication+Date%5D+ AND+Cluzel%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=1066-5277&vol=171& iss=&firstpage=383 http://dx.doi.org/10.1083%2Fjcb.200503017
20. Fitzgerald, L and Poncz, M and Steiner, B and Rall, S, Jr and Bennett, J and Phillips, D. (1987) Comparison of cDNA-derived protein sequences of the human fibronectin and vitronectin receptor alpha-subunits and platelet glycoprotein IIb Biochemistry, 26, pp. 8158-8165. http://www4.infotrieve.com/newmedline/detail.asp?NameID=2450560& Session=&searchQuery=Biochemistry%5BJournal+Name%5D+AND+26% 5BVolume%5D+AND+8158%5BPage+Number%5D+AND+1987%5BPublication+Date%5D+ AND+Fitzgerald%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0006-2979&vol=26& iss=&firstpage=8158 http://dx.doi.org/10.1021%2Fbi00399a021
21. Lum, L and Reid, M and Blobel, C. (1998) Intracellular maturation of the mouse metalloprotease disintegrin MDC15 J Biol Chem, 273, pp. 26236-26247. http://www4.infotrieve.com/newmedline/detail.asp?NameID= 9748307&Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+ AND+273%5BVolume%5D+AND+26236%5BPage+Number%5D+AND+1998%5BPublication+ Date%5D+AND+Lum%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/ linkabst.cgi?issn=0884-3996&vol=273&iss=&firstpage=26236 http://dx.doi.org/10.1074%2Fjbc.273.40.26236
22. Nath, D and Slocombe, P and Stephens, P and Warn, A and Hutchinson, G and Yamada, K and Docherty, A and Murphy, G. (1999) Interaction of metargidin (ADAM-15) with alpha v beta 3 and alpha 5 beta 1 integrins on different haemopoietic cells J Cell Sci, 112, pp. 579-587. http://www4.infotrieve.com/newmedline/detail.asp?NameID=9914169& Session=&searchQuery=J+Cell+Sci%5BJournal+Name%5D+AND+112% 5BVolume%5D+AND+579%5BPage+Number%5D+AND+1999%5BPublication+Date%5D+ AND+Nath%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0269-3518&vol=112& iss=&firstpage=579
23. Xiong, J and Stehle, T and Goodman, S and Arnaout, M. (2004) A novel adaptation of the integrin PSI domain revealed from its crystal structure J Biol Chem, 279, pp. 40252-40254. http://www4.infotrieve.com/newmedline/detail.asp?NameID=15299032& Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+AND+279% 5BVolume%5D+AND+40252%5BPage+Number%5D+AND+2004%5BPublication+Date%5D+ AND+Xiong%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0884-3996&vol=279& iss=&firstpage=40252 http://dx.doi.org/10.1074%2Fjbc.C400362200
24. Mould, A and Symonds, E and Buckley, P and Grossmann, J and McEwan, P and Barton, S and Askari, J and Craig, S and Bella, J and Humphries, M. (2003) Structure of an integrin-ligand complex deduced from solution x-ray scattering and site-directed mutagenesis J Biol Chem, 278, pp. 39993-39999. http://www4.infotrieve.com/newmedline/detail.asp?NameID= 12871973&Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+ AND+278%5BVolume%5D+AND+39993%5BPage+Number%5D+AND+2003%5BPublication+ Date%5D+AND+Mould%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0884-3996&vol=278& iss=&firstpage=39993 http://dx.doi.org/10.1074%2Fjbc.M304627200
25. Xiong, J and Stehle, T and Diefenbach, B and Zhang, R and Dunker, R and Scott, D and Joachimiak, A and Goodman, S and Arnaout, M. (2001) Crystal structure of the extracellular segment of integrin alpha V beta 3 Science, 294, pp. 339-345. http://www4.infotrieve.com/newmedline/ detail.asp?NameID=11546839&Session=&searchQuery=Science% 5BJournal+Name%5D+AND+294%5BVolume%5D+AND+339%5BPage+Number%5D+AND+ 2001%5BPublication+Date%5D+AND+Xiong%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0294-0337&vol=294& iss=&firstpage=339 http://dx.doi.org/10.1126%2Fscience.1064535
26. Pierschbacher, M and Ruoslahti, E and Sundelin, J and Lind, P and Peterson, P. (1982) The cell attachment domain of fibronectin. Determination of the primary structure J Biol Chem, 257, pp. 9593-9597. http://www4.infotrieve.com/newmedline/detail.asp?NameID=7050098& Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+AND+257% 5BVolume%5D+AND+9593%5BPage+Number%5D+AND+1982%5BPublication+Date%5D+ AND+Pierschbacher%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0884-3996&vol=257& iss=&firstpage=9593
27. Kratzschmar, J and Lum, L and Blobel, C. (1996) Metargidin, a membrane-anchored metalloprotease-disintegrin protein with an RGD integrin binding sequence J Biol Chem, 271, pp. 4593-4596. http://www4.infotrieve.com/newmedline/detail.asp?NameID=8617717& Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+AND+271% 5BVolume%5D+AND+4593%5BPage+Number%5D+AND+1996%5BPublication+ Date%5D+AND+Kratzschmar%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0884-3996&vol=271& iss=&firstpage=4593 http://dx.doi.org/10.1074%2Fjbc.271.9.4593
28. Tuckwell, D and Weston, S and Humphries, M. (1993) Integrins: A review of their structure and mechanisms of ligand binding Symp Soc Exp Bio, 89, pp. 107-136. http://www.csa.com/htbin/linkabst.cgi?issn=0081- 1386&vol=89&iss=&firstpage=107
29. Harrington, R. (2004) Controversies surrounding platelet glycoprotein IIb/IIIa inhibitors in percutaneous coronary intervention and acute coronary syndromes Semin Thromb Hemost, 30, pp. 639-647. http://www4.infotrieve.com/newmedline/detail.asp?NameID=15630670& Session=&searchQuery=Semin+Thromb+Hemost%5BJournal+Name%5D+AND+ 30%5BVolume%5D+AND+639%5BPage+Number%5D+AND+2004%5BPublication+ Date%5D+AND+Harrington%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0094-6176&vol=30& iss=&firstpage=639 http://dx.doi.org/10.1055%2Fs-2004-861506
30. Zhang, X and Kamata, T and Yokoyama, K and Puzon-McLaughlin, W and Takada, Y. (1998) Specific interaction of the recombinant disintegrin-like domain of MDC-15 (metargidin, ADAM-15) with integrin alpha v beta 3 J Biol Chem, 273, pp. 7345-7350. http://www4.infotrieve.com/newmedline/detail.asp?NameID=9516430& Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+AND+273% 5BVolume%5D+AND+7345%5BPage+Number%5D+AND+1998%5BPublication+Date%5D+ AND+Zhang%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0884-3996&vol=273& iss=&firstpage=7345 http://dx.doi.org/10.1074%2Fjbc.273.13.7345
31. Eto, K and Puzon-McLaughlin, W and Sheppard, D and Sehara-Fujisawa, A and Zhang, X and Takada, Y. (2000) RGD-independent binding of integrin alpha 9 beta 1 to the ADAM-12 and -15 disintegrin domains mediates cell-cell interaction J Biol Chem, 275, pp. 34922-34930. http://www4.infotrieve.com/newmedline/detail.asp?NameID=10944520& Session=&searchQuery=J+Biol+Chem%5BJournal+Name%5D+AND+275% 5BVolume%5D+AND+34922%5BPage+Number%5D+AND+2000%5BPublication+Date%5D+ AND+Eto%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0884-3996&vol=275& iss=&firstpage=34922 http://dx.doi.org/10.1074%2Fjbc.M001953200
32. Sutcliffe, M and Jaseja, M and Hyde, E and Lu, X and Williams, J. (1994) Three-dimensional structure of the RGD-containing neurotoxin homologue dendroaspin Nat Struct Biol, 1, pp. 802-807. http://www4.infotrieve.com/newmedline/detail.asp?NameID=7634091& Session=&searchQuery=Nat+Struct+Biol%5BJournal+Name%5D+AND+1% 5BVolume%5D+AND+802%5BPage+Number%5D+AND+1994%5BPublication+Date%5D+ AND+Sutcliffe%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=1072-8368&vol=1&iss= &firstpage=802 http://dx.doi.org/10.1038%2Fnsb1194-802
33. Wattam, B and Shang, D and Rahman, S and Egglezou, S and Scully, M and Kakkar, V and Lu, X. (2001) Arg-Tyr-Asp (RYD) and Arg-Cys-Asp (RCD) motifs in dendroaspin promote selective inhibition of beta1 and beta3 integrins Biochem J, 356, pp. 11-17. http://www4.infotrieve.com/ newmedline/detail.asp?NameID=11336631&Session=&searchQuery= Biochem+J%5BJournal+Name%5D+AND+356%5BVolume%5D+AND+11%5BPage+ Number%5D+AND+2001%5BPublication+Date%5D+AND+Wattam%5BAuthor+ Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn= 0264-6021&vol=356&iss=&firstpage=11 http://dx.doi.org/10.1042%2F0264-6021%3A3560011
34. Oshikawa, K and Terada, S. (1999) Ussuristatin 2, A novel KGD-bearing disintegrin from Agkistrodon ussuriensis venom J Biochem (Tokyo), 125, pp. 31-35. http://www.csa.com/htbin/linkabst.cgi?issn=0021- 924X&vol=125&iss=&firstpage=31
35. Sato, S and Abe, T and Tamiya, N. (1970) Binding of iodinated erabutoxin b, a sea snake toxin, to the endplates of the mouse diaphragm Toxicon, 8, pp. 313-314. http://www4.infotrieve.com/newmedline/ detail.asp?NameID=5493741&Session=&searchQuery=Toxicon% 5BJournal+Name%5D+AND+8%5BVolume%5D+AND+313%5BPage+Number%5D+AND+ 1970%5BPublication+Date%5D+AND+Sato%5BAuthor+Name%5D&count=1 http://www.csa.com/htbin/linkabst.cgi?issn=0041-0101&vol=8&iss= &firstpage=313 http://dx.doi.org/10.1016%2F0041-0101%2870%2990010-3
36. Jaseja, M and Lu, X and Williams, J and Sutcliffe, M and Kakkar, V and Parslow, R and Hyde, E. (1994) 1H-NMR assignments and secondary structure of dendroaspin, an RGD-containing glycoprotein IIb-IIIa (alpha IIb-beta 3) antagonist with a neurotoxin fold Eur J Biochem, 226, pp. 861-868. http://www4.infotrieve.com/newmedline/detail.asp?NameID= 7813476&Session=&searchQuery=Eur+J+Biochem%5BJournal+Name%5D+ AND+226%5BVolume%5D+AND+861%5BPage+Number%5D+AND+1994%5BPublication+ Date%5D+AND+Jaseja%5BAuthor+Name%5D&count=1 http://www.csa.com/ htbin/linkabst.cgi?issn=0939-4974&vol=226&iss=&firstpage=861 http://dx.doi.org/10.1111%2Fj.1432-1033.1994.00861.x
37. Titani, K and Maeda, T and Dyama, R and Kimizuka, F and Kato, I and Sekiguchi, K.(1990) Advance in biotechnology and molecular biology.