Modulating the affinity and the selectivity of engineered calmodulin EF-hand peptides for lanthanides

Biotechnology and Bioengineering

Volumn 95, Issue 1, 2006, Pages 29-36

  Le Clainche, Loïc ^a   Figuet, Mélanie ^a   Montjardet Bas, Véronique ^a   Blanchard, Sébastien ^a   Vita, Claudio ^a  

^a DIF   (France)

Author keywords

Biosensors; Calmodulin; EF hand; Fluorescence; Lanthanide binding

Indexed keywords

BINDING ENERGY; BIOSENSORS; CONFORMATIONS; FLUORESCENCE; MUTAGENESIS; PROTEINS; RARE EARTH ELEMENTS; SYNTHESIS (CHEMICAL);

CALMODULIN; CARBOXYLATE RESIDUES; EF-HAND; LANTHANIDE BINDING;

AMINO ACIDS;

AMINO ACID; CALMODULIN; CARBOXYLIC ACID DERIVATIVE; HELIX LOOP HELIX PROTEIN; LANTHANIDE; TERBIUM;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; DISSOCIATION CONSTANT; ELECTROSPRAY MASS SPECTROMETRY; METAL BINDING; NONHUMAN; PARAMECIUM TETRAURELIA; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN MOTIF; PROTEIN SYNTHESIS;

PARAMECIUM TETRAURELIA;

EID: 33748420047     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20946     Document Type: Article

References (26)

1. Babu YS, Bugg CE, Cook WJ. 1988. Structure of calmodulin refined at 2.2 Å resolution. J Mol Biol 204:191-204.
2. Belkin S. 1998. A panel of stress-responsive luminous bacteria for monitoring wastewater toxicity. Methods Mol Biol 102:247-258.
3. Bonn G, Ruzza P, Rossi M, Calderan A, Marchiori F, Peggion E. 1989. Synthesis of the dodecapeptide corresponding to domain III of bovine brain calmodulin: Alpha-beta shift side reactions during the synthesis by the classical method in solution. Biopolymers 28:353-369.
4. 2+ dications and with a target enzyme peptide. J Chem Phys 119:1874-1878.
5. Dadlez M, Goral J, Bierzynski A. 1991. Luminescence of peptide-bound terbium ions. Determination of binding constants. FEBS Lett 282:143-146.
6. Giuliano KA, Post PL, Hahn KM, Taylor DL. 1995. Fluorescent protein biosensors: Measurement of molecular dynamics in living cells. Annu Rev Biophys Biomol Struct 24:405-434.
7. Gu MB, Chang TS. 2001. Soil biosensor for the detection of PAH toxicity using an immobilized recombinant bacterium and a biosurfactant. Biosen Bioelectron 16:667-674.
8. Kuzmic P. 1996. Program DYNAFIT for the analysis of enzyme kinetic data: Application to HIV protease. Anal Biochem 237:260-273.
9. Le Clainche L, Plancque G, Amekraz B, Moulin C, Pradines-Lecomte C, Peltier G, Vita C. 2003. Engineering new metal specificity in EF-Hand peptides. J Biol Inorg Chem 8:334-340.
10. Loo JA. 1997. Studying noncovalent protein complexes by electrospray ionization mass spectrometry. Mass Spectrom Rev 16:1-23.
11. Marsden BJ, Shaw GS, Sykes BD. 1990. Calcium binding proteins. Elucidating the contributions to calcium affinity from an analysis of species variants and peptide fragments. Biochem Cell Biol 68:587-601.
12. McPhalen CA, Strynadka NC, James MN. 1991. Calcium binding sites in proteins: A structural perspective. Adv Protein Chem 42:77-144.
13. Mezo G, Drakopoulou E, Paal V, Rajnavolgyi E, Vita C, Hudecz F. 2000. Synthesis and immunological studies of alpha-conotoxin chimera containing an immunodominant epitope from the 268-284 region of HSV gD protein. J Pept Res 55:7-17.
14. 2+ based on green fluorescent proteins and calmodulin. Nature 388:882-887.
15. Moulin V, Tits J, Moulin C, Decambox P, Mauchien P, de Ruty O. 1992. Complexation behaviour of humic substances towards actinides and lanthanides studied by time-resolved laser induced spectrofluorimetry. Radiochim Acta 58:121-128.
16. Nitz M, Franz KJ, Maglathin RL, Imperiali B. 2003. A powerful combinatorial screen to identify high-affinity terbium(III)-binding peptides. Chem Bio Chem 4:272-276.
17. Nitz M, Sherawat M, Franz KJ, Peisach E, Allen KN, Imperiali B. 2004. Structural origin of the high affinity of a chemically evolved lanthanide-binding peptide. Angew Chem Int Ed Engl 43:3682-3685.
18. Procyshyn RM, Reid RE. 1994. A structure/activity study of calcium affinity and selectivity using a synthetic peptide model of the helix-loop-helix calcium-binding motif. J Biol Chem 269:1641-1647.
19. Rao ST, Satyshur KA, Greaser ML, Sundaralingam M. 1996. X-ray structures of Mn, Cd and Tb metal complexes of troponin C. Acta Crystallogr D Biol Crystallogr 52:916.
20. Richardson JS. 1981. The anatomy and taxonomy of protein structure. Adv Protein Chem 34:167-339.
21. Rogers KR. 1995. Biosensors for environmental applications. Biosens Bioelectron 10:533-541.
22. Tam JP, Riemen MW, Merriefield RB. 1988. Mechanisms of aspartimide formation: The effects of protecting groups, acid, base, temperature and time. Pept Res 1:6-18.
23. Tsien RY. 1993. Fluorescent and photochemical probes of dynamic biochemical signals inside living cells. Czarnik AW, editor. Fluorescent Chemosensors for Ion and Molecule Recognition. Washington DC: American Chemical Society. p 130-146.
24. 2+-bound calmodulin: An analysis of disorder and implications for functionaly relevant plasticity. J Mol Biol 301:1237-1256.
25. Wu X, Reid RE. 1997. Conservative D133E mutation of calmodulin site IV drastically alters calcium binding and phosphodiesterase regulation. Biochemistry 36:8649-8656.
26. Ye Y, Lee HW, Yang W, Yang JJ. 2005. Calcium and lanthanide affinity of the EF-loops from the C-terminal domain of calmodulin. J Inorg Biochem 99:1376-1383.