Tricarboxylic acid cycle intermediates accumulate at the onset of intense exercise in man but are not essential for the increase in muscle oxygen uptake

Pflugers Archiv European Journal of Physiology

Volumn 452, Issue 6, 2006, Pages 737-743

  Bangsbo, Jens ^a,c   Gibala, Martin J ^b   Howarth, Krista R ^b   Krustrup, Peter ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b MCMASTER UNIVERSITY   (Canada)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Alanine; Creatine phosphate; Dichloracetate; Lactate; Oxygen uptake

Indexed keywords

ALANINE; CITRIC ACID; CREATINE PHOSPHATE; DICHLOROACETIC ACID; FUMARIC ACID; MALIC ACID;

ADULT; ARTICLE; CITRIC ACID CYCLE; CONTROLLED STUDY; EXERCISE PHYSIOLOGY; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; MALE; METABOLITE; MUSCLE BIOPSY; NORMAL HUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; SKELETAL MUSCLE; VASTUS LATERALIS MUSCLE;

ADULT; ALANINE; BIOPSY; CITRIC ACID CYCLE; DICHLOROACETATE; ENERGY METABOLISM; EXERCISE; EXERCISE TEST; HUMANS; KINETICS; KNEE; MALE; MUSCLE, SKELETAL; OXIDATION-REDUCTION; OXYGEN CONSUMPTION; SUPINE POSITION;

EID: 33748339775     PISSN: 00316768     EISSN: 14322013     Source Type: Journal    
DOI: 10.1007/s00424-006-0075-4     Document Type: Article

References (40)

1. Andersen P, Adams RP, Sjøgaard G, Thorboe A, Saltin B (1985) Dynamic knee extension as a model for the study of an isolated exercising muscle in man. J Appl Physiol 59:1647-1653
2. 2 deficit-debt relationship during exhaustive exercise in humans. J Physiol 422:539-559
3. Bangsbo J, Krustrup P, González-Alonso J, Saltin B (2000) Muscle oxygen kinetics at onset of intense dynamic exercise in humans. Am J Physiol 279(3):R899-R906
4. Bangsbo J, Krustrup P, González-Alonso J, Saltin B (2001) ATP production and mechanical efficiency of human skeletal muscle during intense exercise: effect of previous exercise. Am J Physiol 280:E956-E964
5. 2 uptake at onset of intense exercise in humans. Am J Physiol 282:R273-R280
6. Bergmeyer HU (1974) Methods of enzymatic analysis, 2nd edn. Academic, New York
7. Bergström J (1962) Muscle electrolytes in man. Scand J Clin Lab Invest Suppl 68:1-101
8. Chung Y, Mole PA, Sailasuta N, Tran TK, Hurd R, Jue T (2005) Control of respiration and bioenergetics during muscle contraction. Am J Physiol 288(3):C730-C738
9. Constantin-Teodosiu D, Simpson EJ, Greenhaff PL (1998) Effects of pyruvate, dichloroacetate and adrenaline infusion on pyruvate dehydrogenase complex activation and tricarboxylic acid cycle intermediates in human skeletal muscle. J Physiol 506:102P
10. Dawson KD, Howarth KR, Tarnopolsky MA, Wong ND, Gibala MJ (2003) Short-term training attenuates muscle TCA cycle expansion during exercise in women. J Appl Physiol 95(3):999-1004
11. Dawson KD, Baker DJ, Greenhaff PL, Gibala MJ (2005) An acute decrease in TCA cycle intermediates does not affect aerobic energy delivery in contracting rat skeletal muscle. J Physiol 565(2):637-643
12. Gibala MJ, Saltin B (1999) PDH activation by dichloroacetate reduces TCA cycle intermediates at rest but not during exercise in humans. Am J Physiol 277(1):E33-E38
13. Gibala MJ, Tarnopolsky MA, Graham TE (1997) Tricarboxylic acid cycle intermediates in human muscle at rest and during prolonged cycling. Am J Physiol 272(1):E239-E244
14. Gibala MJ, MacLean DA, Graham T, Saltin B (1998) Tricarboxylic acid cycle intermediate pool size and estimated cycle flux in human muscle during exercise. Am J Physiol 275(2):E235-E242
15. Gibala MJ, Young ME, Taegtmeyer H (2000) Anaplerosis of the citric acid cycle: role in energy metabolism of heart and skeletal muscle. Acta Physiol Scand 168(4):657-665
16. Gibala MJ, González-Alonso J, Saltin B (2002) Dissociation between muscle tricarboxylic acid cycle pool size and aerobic energy provision during prolonged exercise in humans. J Physiol 545(2):705-713
17. Grassi B (2000) Skeletal muscle VO2 on-kinetics: set by O2 delivery or by O2 utilization? New insights into an old issue. Med Sci Sports Exerc 32(1):108-116
18. 2 uptake kinetics in humans: implications for metabolic control. J Appl Physiol 80:988-998
19. 2 on-kinetics in isolated in situ canine muscle. J Appl Physiol 85(4):1394-1403
20. 2 on-kinetics in isolated in situ canine muscle. J Appl Physiol 85(4):1404-1412
21. Harris RC, Hultman E, Nordesjö O (1974) Glycogen, glycolytic intermediates and high-energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest. Scand J Clin Lab Invest 33:109-120
22. Howarth KR, LeBlanc PJ, Heigenhauser GJ, Gibala MJ (2004) Effect of endurance training on muscle TCA cycle metabolism during exercise in humans. J Appl Physiol 97(2):579-584
23. Howlett RA, Heigenhauser GJ, Spriet LL (1999) Skeletal muscle metabolism during high-intensity sprint exercise is unaffected by dichloroacetate or acetate infusion. J Appl Physiol 87(5):1747-1751
24. 2 on blood flow of forearm exercise. J Appl Physiol 81(4):1516-1521
25. Hughson RL, Tschakovsky ME, Houston ME (2001) Regulation of oxygen consumption at the onset of exercise. Exerc Sport Sci Rev 29(3):129-133
26. 2) on-kinetics in skeletal muscles. Biochem J 379(3):703-710
27. Krustrup P, González-Alonso J, Quistorff B, Bangsbo J (2001) Muscle heat production and anaerobic energy turnover during repeated intense dynamic exercise in humans. J Physiol 536:947-956
28. Krustrup P, Hellsten Y, Bangsbo J (2004) Intense interval training enhances human skeletal muscle oxygen uptake in the initial phase of dynamic exercise at high but not at low intensities. J Physiol 559(1):335-345
29. Lee SH, Davis EJ (1979) Carboxylation and decarboxylation reactions. Anaplerotic flux and removal of citrate cycle intermediates in skeletal muscle. J Biol Chem 254(2):420-430
30. Ludvik B, Mayer G, Stifter S, Putz D, Barnas U, Graf H (1993) Effects of dichloroacetate on exercise performance in healthy volunteers. Pflugers Arch 423(3-4):251-254
31. Messer JI, Jackman MR, Willis WT (2004) Pyruvate and citric acid cycle carbon requirements in isolated skeletal muscle mitochondria. Am J Physiol 286:C565-C572
32. Molé PA, Chung Y, Tran TK, Sailasuta N, Hurd R, Jue T (1999) Myoglobin desaturation with exercise intensity in human gastrocnemius muscle. Am J Physiol 277(1):R173-R180
33. Passonneau JV, Lowry OH (1993) Enzymatic analysis: a practical guide. Humana Press, Totowa, NJ
34. Sahlin K, Katz A, Broberg S (1990) Tricarboxylic acid cycle intermediates in human muscle during prolonged exercise. Am J Physiol 259:C834-C841
35. Stacpoole PW (1989) The pharmacology of dichloroacetate. Metabolism 38(11):1124-1144
36. Timmons JA, Gustafsson T, Sundberg CJ, Jansson E, Greenhaff PL (1998) Muscle acetyl group availability is a major determinant of oxygen deficit in humans during submaximal exercise. Am J Physiol 274:E377-E380
37. Timmons JA, Poucher SM, Constantin-Teodosiu D, Macdonald IA, Greenhaff PL (1998) Regulation of skeletal muscle carbohydrate oxidation during steady-state contraction. Am J Physiol 274:R1384-R1389
38. Vicini P, Kushmerick MJ (2000) Cellular energetics analysis by a mathematical model of energy balance: estimation of parameters in human skeletal muscle. Am J Physiol 279(1):C213-C224
39. Walsh B, Tonkonogi M, Söderlund K, Hultman E, Saks V, Sahlin K (2001) The role of phosphorylcreatine and creatine in the regulation of mitochondrial respiration in human skeletal muscle. J Physiol 537(3):971-978
40. Williamson JR, Cooper RH (1980) Regulation of the citric acid cycle in mammalian systems. FEBS Lett 117:K73-K83 (Suppl)