Structural basis of glmS ribozyme activation by glucosamine-6-phosphate

Science

Volumn 313, Issue 5794, 2006, Pages 1752-1756

  Klein, Daniel J ^a   Ferré D'Amaré, Adrian R ^a  

^a Fred Hutchinson Cancer Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYST ACTIVITY; ELECTROSTATICS; ENZYMES; GLUCOSE;

COENZYME BINDING; RIBOSWITCHES;

RNA;

GLUCOSAMINE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE; MESSENGER RNA; PROTEIN GLMS; RIBOZYME; UNCLASSIFIED DRUG;

CATALYSIS; RNA;

ACID BASE BALANCE; ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; PRIORITY JOURNAL; RNA CLEAVAGE; RNA CONFORMATION; RNA STRUCTURE;

5' UNTRANSLATED REGIONS; BASE PAIRING; BASE SEQUENCE; BINDING SITES; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ENZYME ACTIVATION; ENZYME INHIBITORS; GLUCOSAMINE; GLUCOSE-6-PHOSPHATE; GLUTAMINE-FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING); HYDROGEN BONDING; LIGANDS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RNA, CATALYTIC; THERMOANAEROBACTER;

EID: 33748325570     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1129666     Document Type: Article

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38. We thank the staff at Advanced Light Source beamlines 5.0.1 and 5.0.2 and J. Bolduc for assistance with synchrotron and in-house x-ray data collection, respectively, and M. Been, R. Breaker, T. Edwards, T. Hamma, B. Kaiser, R. Klein, J. Pitt, J. Pizzarro, A. Roll-Mecak, B. Shen, J. Simon, G. Varani, and Y. Xiong for discussions. A.R.F. is a Distinguished Young Scholar in Medical Research of the W. M. Keck Foundation. D.J.K. is a Damon Runyon Fellow supported by the Damon Runyon Cancer Research Foundation (DRG-1863-0S). This work was also supported by grants from the NIH (GM63576) and the W. M. Keck Foundation (both to A.R.F.). Atomic coordinates and experimental structure factor amplitudes for the 2′-amino and 2′-deoxy precleavage structures, the Glc6P-bound 2′-deoxy precleavage structure, and the postcleavage structure have been deposited with the Protein Data Bank under the accession codes 2GCS, 2HOS, 2HOZ, and 2GCV, respectively. The 2′-amino precleavage and the postcleavage structures obtained from crystals grown in the absence of GlcN6P have accession codes 2H0X and 2H0W, respectively. The structures obtained by soaking all-RNA crystals with Glc6P and GlcN6P have accession codes 2HO7 and 2HO6, respectively.