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14
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33748312680
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note
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A few microlitres of a lipid solution (10mg/ml of lipids in chloroform) were spread on a hydrophilic glass slide and left in a vacuum chamber overnight. The dry lipid film was then rehydrated with milliQ water.
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15
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33748318958
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note
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Systematic spotting of vesicles was made by edge detection. Closed boundaries were filled in to make uniform objects; the initial image was turned into a binary picture. Feret diameters were measured in four directions to check the isotropy of the result. Finally, objects with a form factor larger than 1.2 were not taken into account to avoid any artifact (stuck vesicles, small defects,...)
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17
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33748293735
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note
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As mentioned by a referee, this could explain the low size dispersion of the vesicles.
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18
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edited by TIEN H. T. and OTTOVA-LEITMANNOVA A. (Elsevier, Amsterdam)
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