A protocol for combined delipidation and subfractionation of membrane proteins using organic solvents

Analytical Biochemistry

Volumn 357, Issue 1, 2006, Pages 144-146

  Schröder, Bernd ^a   Hasilik, Andrej ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

DELIPIDATION; MEMBRANE PROTEINS;

PROTEINS;

ANAZOLENE SODIUM; CETRIMIDE; CHLOROFORM; DITHIOTHREITOL; GLYCEROL; IODOACETAMIDE; METHANOL; ORGANIC SOLVENT; PHOSPHOLIPID; PROTEIN; TRIFLUOROACETIC ACID; TROMETAMOL;

ARTICLE; HYDROPHOBICITY; ISOELECTRIC FOCUSING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PHASE SEPARATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; SOLUBILIZATION; TWO DIMENSIONAL ELECTROPHORESIS; ULTRACENTRIFUGATION;

EID: 33748297808     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.05.035     Document Type: Article

References (8)

1. Diettrich O., Gallert F., and Hasilik A. Purification of lysosomal membrane proteins from human placenta. Eur. J. Cell Biol. 69 (1996) 99-106
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
3. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
4. Heukeshoven J., and Dernick R. Improved silver staining procedure for fast staining in PhastSystem Development Unit: I. Staining of sodium dodecyl sulfate gels. Electrophoresis 9 (1988) 28-32
5. Buxbaum E. Cationic electrophoresis and electrotransfer of membrane glycoproteins. Anal. Biochem. 314 (2003) 70-76
6. Wessel D., and Flugge U.I. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal. Biochem. 138 (1984) 141-143
7. Ferro M., Seigneurin-Berny D., Rolland N., Chapel A., Salvi D., Garin J., and Joyard J. Organic solvent extraction as a versatile procedure to identify hydrophobic chloroplast membrane proteins. Electrophoresis 21 (2000) 3517-3526
8. Molloy M.P., Herbert B.R., Williams K.L., and Gooley A.A. Extraction of Escherichia coli proteins with organic solvents prior to two-dimensional electrophoresis. Electrophoresis 20 (1999) 701-704