Cloning of Dictyostelium eIF6 (p27BBP) and mapping its nucle(ol)ar localization subdomains

European Journal of Cell Biology

Volumn 85, Issue 9-10, 2006, Pages 1069-1078

  Balbo, Alessandra ^a   Bozzaro, Salvatore ^a  

^a UNIVERSITY OF TURIN   (Italy)

Author keywords

Dictyostelium discoideum; eIF6; Green fluorescent protein; Nuclear localization; Nucleolar localization; Nucleolus; Slime molds

Indexed keywords

BIOLOGICAL MARKER; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INITIATION FACTOR; INITIATION FACTOR 6; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; DICTYOSTELIUM; DOWN REGULATION; GENE ACTIVATION; GENE DELETION; GENE EXPRESSION; GENE LOCATION; GENE MAPPING; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; NUCLEOLUS; NUCLEOTIDE SEQUENCE; PHAGOCYTOSIS; PINOCYTOSIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DOMAIN; SEQUENCE ANALYSIS; STARVATION; UPREGULATION;

DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM; EUKARYOTA; MYXOGASTRIA;

EID: 33748285222     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejcb.2006.05.010     Document Type: Article

References (35)

1. Adachi H., Hasebe T., Yoshinaga K., Ohta T., and Sutoh K. Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker. Biochem. Biophys. Res. Commun. 205 (1994) 1808-1814
2. Alton T.H., and Lodish H.F. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Dev. Biol. 60 (1977) 180-206
3. Arigoni M., Bracco E., Lusche D.F., Kae H., Weeks G., and Bozzaro S. A novel Dictyostelium RasGEF required for chemotaxis and development. BMC Cell Biol. 6 (2005) 43
4. Basu U., Si K., Warner J.R., and Maitra U. The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis. Mol. Cell. Biol. 21 (2001) 1453-1462
5. Benichou J.C., Quiviger B., and Ryter A. Cytochemical study of the nucleolus of the slime mold Dictyostelium discoideum. J. Ultrastruct. Res. 84 (1983) 60-66
6. Biffo S., Sanvito F., Costa S., Preve L., Pignatelli R., Spinardi L., and Marchisio P.C. Isolation of a novel beta4 integrin-binding protein (p27(BBP)) highly expressed in epithelial cells. J. Biol. Chem. 272 (1997) 30314-30321
7. Bozzaro S., and Gerisch G. Contact sites in aggregating cells of Polysphondylium pallidum. J. Mol. Biol. 120 (1978) 265-279
8. Bozzaro S., Merkl R., and Gerisch G. Cell adhesion: its quantification, assay of the molecules involved, and selection of defective mutants in Dictyostelium and Polysphondylium. Methods Cell Biol. 28 (1987) 359-385
9. + ATPase B subunit of Dictyostelium discoideum. FEBS Lett. 419 (1997) 37-40
10. Carotenuto R., De Marco N., Biffo S., Wilding M., Vaccaro M.C., Marchisio P.C., Capriglione T., Russo G.L., and Campanella C. Phosphorylation of p27(BBP)/eIF6 and its association with the cytoskeleton are developmentally regulated in Xenopus oogenesis. Cell. Mol. Life Sci. 62 (2005) 1641-1652
11. Ceci M., Gaviraghi C., Gorrini C., Sala L.A., Offenhauser N., Marchisio P.C., and Biffo S. Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly. Nature 426 (2003) 579-584
12. Faix J., Dittrich W., Prassler J., Westphal M., and Gerisch G. pDcsA vectors for strictly regulated protein synthesis during early development of Dictyostelium discoideum. Plasmid 34 (1995) 148-151
13. Groft C.M., Beckmann R., Sali A., and Burley S.K. Crystal structures of ribosome anti-association factor IF6. Nat. Struct. Biol. 7 (2000) 1156-1164
14. Ken R., and Singleton C.K. Redundant regulatory elements account for the developmental control of a ribosomal protein gene of Dictyostelium discoideum. Differentiation 55 (1994) 97-103
15. Kessin R.H. RNA metabolism during vegetative growth and morphogenesis of the cellular slime mold Dictyostelium discoideum. Dev. Biol. 31 (1973) 242-251
16. Liu J., Du X., and Ke Y. Mapping nucleolar localization sequences of 1A6/DRIM. FEBS Lett. 580 (2006) 1405-1410
17. MacLean N., Garside K., Bradley M.C., and Wood C. The nucleus of axenically grown Dictyostelium discoideum: studies on its division cycle and conformation. Experientia 40 (1984) 1207-1214
18. Margolskee J.P., Froshauer S., Skrinska R., and Lodish H.F. The effects of cell density and starvation on early developmental events in Dictyostelium discoideum. Dev. Biol. 74 (1980) 409-421
19. Moerman A.M., and Klein C. Dictyostelium discoideum Hsp32 is a resident nucleolar heat-shock protein. Chromosoma 107 (1998) 145-154
20. Noegel A., Gerisch G., Stadler J., and Westphal M. Complete sequence and transcript regulation of a cell adhesion protein from aggregating Dictyostelium cells. EMBO J. 5 (1986) 1473-1476
21. Pang K.M., Lynes M.A., and Knecht D.A. Variables controlling the expression level of exogenous genes in Dictyostelium. Plasmid 41 (1999) 187-197
22. Peracino B., Borleis J., Jin T., Westphal M., Schwartz J.M., Wu L.J., Bracco E., Gerisch G., Devreotes P., and Bozzaro S. G protein beta subunit-null mutants are impaired in phagocytosis and chemotaxis due to inappropriate regulation of the actin cytoskeleton. J. Cell Biol. 141 (1998) 1529-1537
23. Peracino B., Wagner C., Balest A., Balbo A., Pergolizzi B., Noegel A., Steinert M., and Bozzaro S. Function and mechanism of action of Dictyostelium Nramp1 (Slc11a1) in bacterial infection. Traffic 7 (2006) 22-38
24. Pergolizzi B., Peracino B., Silverman J., Ceccarelli A., Noegel A., Devreotes P., and Bozzaro S. Temperature-sensitive inhibition of development in Dictyostelium due to a point mutation in the piaA gene. Dev. Biol. 251 (2002) 18-26
25. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: a Laboratory Manual. second ed (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
26. Sameshima M. The orientation of nucleus, nucleus-associated body and protruding nucleolus in aggregating Dictyostelium discoideum. Exp. Cell Res. 156 (1985) 341-350
27. Sanvito F., Piatti S., Villa A., Bossi M., Lucchini G., Marchisio P.C., and Biffo S. The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly. J. Cell Biol. 144 (1999) 823-837
28. Schofer C., Weipoltshammer K., Almeder M., Muller M., and Wachtler F. Redistribution of ribosomal DNA after blocking of transcription induced by actinomycin D. Chromosome Res. 4 (1996) 384-391
29. Senger B., Lafontaine D.L., Graindorge J.S., Gadal O., Camasses A., Sanni A., Garnier J.M., Breitenbach M., Hurt E., and Fasiolo F. The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis. Mol. Cell 8 (2001) 1363-1373
30. Si K., and Maitra U. The Saccharomyces cerevisiae homologue of mammalian translation initiation factor 6 does not function as a translation initiation factor. Mol. Cell. Biol. 19 (1999) 1416-1426
31. Watts D.J., and Ashworth J.M. Growth of myxamoebae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem. J. 119 (1970) 171-174
32. Westphal M., Jungbluth A., Heidecker M., Muhlbauer B., Heizer C., Schwartz J.M., Marriott G., and Gerisch G. Microfilament dynamics during cell movement and chemotaxis monitored using a GFP-actin fusion protein. Curr. Biol. 7 (1997) 176-183
33. Wood L.C., Ashby M.N., Grunfeld C., and Feingold K.R. Cloning of murine translation initiation factor 6 and functional analysis of the homologous sequence YPR016c in Saccharomyces cerevisiae. J. Biol. Chem. 274 (1999) 11653-11659
34. Wu L.J., Valkema R., van Haastert P.J.M., and Devreotes P.N. The G protein beta subunit is essential for multiple responses to chemoattractants in Dictyostelium. J. Cell Biol. 129 (1995) 1667-1675
35. Xue Z., and Melese T. Nucleolar proteins that bind NLSs: a role in nuclear import or ribosome biogenesis?. Trends Cell Biol. 4 (1994) 414-417