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Volumn 32, Issue 10, 1993, Pages 1432-1434

Synthesis and Pairing Properties of Decanucleotides from (3′S,5′R)‐2′‐Deoxy‐3′, 5′‐ethanoβ‐D‐ribofuranosyladenine and ‐thymine

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Indexed keywords


EID: 33748231655     PISSN: 05700833     EISSN: 15213773     Source Type: Journal    
DOI: 10.1002/anie.199314321     Document Type: Article
Times cited : (80)

References (33)
  • 18
    • 84989571681 scopus 로고    scopus 로고
    • 3S.
  • 20
    • 84989576319 scopus 로고    scopus 로고
    • Oligonucleotide synthesis was performed on a Pharmacia Gene Assembler Plus DNA synthesizer in scales of 0.6–1.3 mmol. The only parameters changed relative to the synthesis of natural DNA oligomers were the detritylation time (extended to 60 s) and the coupling time (extended to 6 min). The last step in every synthesis was the removal of the 5′‐terminal trityl group (trityl off mode).
  • 21
    • 84989564131 scopus 로고    scopus 로고
    • 3NHOAc, pH 7.0). Mass spectrometric analysis of 1 and 2 (NH 4+ form) was performed by matrix‐assisted laser desorption time of flight mass spectroscopy (m/z > 3240.8 1), 3330.8 (2).
  • 22
    • 0013947253 scopus 로고
    • 10 under the buffer conditions used for recording the UV melting curves. This is in accordance with previous findings on the pairing behavior of poly(dA) with poly(U)
    • (1966) J. Mol. Biol. , vol.20 , pp. 359-389
    • Riley, M.1    Maling, B.2    Chamberlin, M.J.3
  • 26
    • 84989571701 scopus 로고    scopus 로고
    • 10)were also obtained by differential scanning calorimetry on a Microcal MC‐2D instrument (1 M NaCl). A (numerical) entropy reduction of 23% in the former duplex relative to the latter was observed.
  • 27
    • 84989540660 scopus 로고    scopus 로고
    • 10), respectively.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.