An optimal pooling strategy applied to high-throughput screening for rare marker-free transformants

Biotechnology Letters

Volumn 28, Issue 19, 2006, Pages 1537-1544

  Zhang, Jinrui ^a   Xiao, Qin ^a   Li, Kexiu ^a,b   Chen, Mingjie ^a   Chang, Junli ^a   Luo, Liting ^a   Li, Yang ^a   Liu, Yong ^a   Shewry, Peter R ^b   He, Guangyuan ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

Accurate and Fast Target Screening; Group testing; Marker free transformation; Transgene expression; Transgene integration

Indexed keywords

ACCURATE AND FAST TARGET SCREENING; GROUP TESTING; MARKER-FREE TRANSFORMATION; TRANSGENE EXPRESSION; TRANSGENE INTEGRATION;

BIOMARKERS; ELECTROPHORESIS; GENES; PROTEINS; SURFACE ACTIVE AGENTS;

GENETIC ENGINEERING;

GLUTEN; GLUTENIN; RECOMBINANT DNA;

ALGORITHM; ARTICLE; CELL CULTURE; CHEMISTRY; GENETIC MARKER; GENETIC TRANSFORMATION; GENETICS; METABOLISM; PLANT SEED; POLYMERASE CHAIN REACTION; TRANSGENE; TRANSGENIC PLANT; WHEAT;

ALGORITHMS; CELLS, CULTURED; DNA, RECOMBINANT; GENETIC MARKERS; GLUTEN; GLUTENS; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; SEEDS; TRANSFORMATION, GENETIC; TRANSGENES; TRITICUM;

TRITICUM AESTIVUM;

EID: 33748122679     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9129-0     Document Type: Article

References (15)

1. Barro F, Rooke L, Békés F, Gras P, Tatham AS, Fido RJ, Lazzeri PA, Shewry PR, Barcelo P (1997) Transformation of wheat with high molecular weight subunit genes results in improved functional properties. Nat Biotechnol 15:1295-1299
2. Chen C, Swallow W (1990) Using group testing to estimate a proportion, and to test the binomial model. Biometrics 46:1035-1046
3. Corneille S, Lutz K, Svab Z, Maliga P (2001) Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system. Plant J 27:171-178
4. Daniell H (2002) Molecular strategies for gene containment in transgenic crops. Nat Biotechnol 20:581-586
5. Du DZ, Hwang FK (2000) Combinatorial group testing, 2nd edn. World Scientific, Singapore
6. Gomord V, Faye L (2004) Posttranslational modification of therapeutic proteins in plants. Curr Opin Plant Biol 7:171-182
7. Halford NG, Field JM, Blair H, Urwin P, Moore K, Robert L, Thompson R (1992) Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theor Appl Genet 83:373-378
8. He GY, Rooke L, Steele S, Békés F, Gras P, Tatham AS, Fido RJ (1999) Transformation of pasta wheat (Triticum turgidum L. var. durum) with high molecular weight glutenin subunit genes and modification of dough functionality. Mol Breed 5:377-386
9. Hung M, Swallow W (1999) Robustness of group testing in the estimation of proportions. Biometrics 55:231-237
10. Klaus SMJ, Huang FC, Golds TJ, Koop H-U (2004) Generation of marker-free plastid transformants using a transiently cointegrated selection gene. Nat Biotechnol 22:225-229
11. Love DR, Pichler FB, Dodd A, Copp BR, Greenwood DR (2004) Technology for high-throughput screens: the present and future using zebrafish. Curr Opin Plant Biol 15:564-572
12. Nikolov ZL, Woodard SL (2004) Downstream processing of recombinant proteins from transgenic feedstock. Curr Opin Biotechnol 15(5):479-486
13. Stacey J, Isaac PG (1994) Isolation of DNA from plants. In: Isaac PG (ed) Methods in molecular biology: protocols for nucleic acid analysis by nonradioactive probes. Humana Press Inc
14. Shewry PR, Tatham AS, Fido RJ (1995) Separation of plant proteins by electrophoresis. In: Jones H (ed) Methods in molecular biology: plant gene transfer and expression protocols, vol 49. Humana Press Inc., pp 423-437
15. Tebbs J, Swallow W (2003) Estimating ordered binomial proportions with the use of group testing. Biometrika 90:471-477