Development of real-time-PCR assays for detection and characterization of Clostridium perfringens in food samples;Etablierung von real-time-PCR-verfahren für den nachweis und die charakterisierung von Clostridium perfringens in lebensmitteln

Archiv fur Lebensmittelhygiene

Volumn 57, Issue 4, 2006, Pages 102-105

  Messelhäußer, Ute ^a   Ziegler, Helene ^a   Elmer Englhard, Damaris ^a   Busch, Ulrich ^a   Hörmansdorfer, Stefan ^a   Kahlau, Dagmar ^a   Pudich, Ursula ^a   Höller, Christiane ^a  

^a Dienststelle Oberschleißheim ^*  (Germany)

Author keywords

Clostridium perfringens; Food; Real time PCR

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); CLOSTRIDIUM PERFRINGENS; POSIBACTERIA;

EID: 33748066013     PISSN: 0003925X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. BVET (2005): Clostridium perfringens: Clostridien-Gastroenteritis. BVET, März 2005.
2. Daube, G., P. Simon, B. Limbourg, C. Manteca, J. Mainil, A. Kaeckenbeeck (1996): Hybridization of 2,659 Clostridium perfringens isolates with gene probes for seven toxins (α, β, ε, ι, θ, μ and enterotoxin) and for sialidase. Am. J. Vet. Res. 57, 496-501.
3. DIN EN ISO 7937. (2004): Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony count technique.
4. Eisgruber, H. (1996): Zur lebensmittelhygienischen Bedeutung von Clostridium perfringens. Arch. Lebensmittelhyg. 47, 57-80.
5. Hausschild, A.H.W., R. Hilsheimer (1974): Evaluation and modification of media for enumeration of Clostridium perfringens. Appl. Microbiol. 27, 78-82.
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8. Petit, L., M. Gibert, M.R. Popoff (1999): Clostridium perfringens: toxinotype and genotype. Trends Microbiol. 7, 104-110.
9. Songer, J.G., D. Bueschel (1999): Multiplex procedure for genotyping Clostridium perfringens. www.microvet.arizona.edu/Faculty/Songer/ multiplexprocedure.pdf
10. Sparks, S.G., R.J. Carman, M.R. Sarker, B.A. McClane (2001): Genotyping of enterotoxigenic Clostridium perfringens isolates associated with gastrointestinal disease in North America. J. Clin. Microbiol. 39, 883-888.
11. Wen, Q., K. Miyamoto, B.A. McClane (2003): Development of a duplex PCR genotyping assay for distinguishing Clostridium perfringes type A isolates carrying chromosomal enterotoxin (cpe) genes from those carrying plasmid-borne enterotoxin (cpe) genes. J. Clin. Microbiol. 41, 1494-1498.