Cloning of full-length cDNA of teleost corticotropin-releasing hormone precursor by improved inverse PCR

Bioscience, Biotechnology and Biochemistry

Volumn 70, Issue 8, 2006, Pages 1983-1986

  Kuniyoshi, Hisato ^a   Fukui, Yukio ^a   Sakai, Yoichi ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Corticotropin releasing hormone precursor; Halichoeres tenuispinnis; Inverse PCR; Teleost

Indexed keywords

CHROMOSOMES; CLONING; DNA; MICROORGANISMS; REACTION KINETICS; RNA;

CORTICOTROPIN RELEASING HORMONE PRECURSOR; HALICHOERES TENUISPINNIS; INVERSE PCR; TELEOST;

HORMONES;

COMPLEMENTARY DNA; CORTICOTROPIN RELEASING FACTOR; MESSENGER RNA; PROTEIN PRECURSOR;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; GENE EXPRESSION PROFILING; GENETICS; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PERCIFORMES; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CORTICOTROPIN-RELEASING HORMONE; DNA, COMPLEMENTARY; GENE EXPRESSION PROFILING; MOLECULAR SEQUENCE DATA; PERCIFORMES; POLYMERASE CHAIN REACTION; PROTEIN PRECURSORS; RNA, MESSENGER;

TELEOSTEI;

EID: 33747861348     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.60067     Document Type: Article

References (7)

1. Frohman, M. A., Dush, M. K., and Martin, G. R., Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA, 85, 8998-9002 (1988).
2. Schaefer, B. C., Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal. Biochem., 227, 255-273 (1995).
3. Friedrich, M., Grahnert, A., and Hauschildt, S., Analysis of the 3′ UTR of the ART3 and ART4 gene by 3′ inverse RACE-PCR. DNA Seq., 16, 53-57 (2005).
4. Huang, S. H., Hu, Y. Y., Wu, C. H., and Holcenberg, J., A simple method for direct cloning cDNA sequence that flanks a region of known sequence from total RNA by applying the inverse polymerase chain reaction. Nucl. Acids Res., 18, 1922 (1990).
5. Maruyama, I. N., Rakow, T. L., and Maruyama, H. I., cRACE: a simple method for identification of the 5′ end of mRNAs. Nucl. Acids Res., 23, 3796-3797 (1995).
6. Zhu, Y. Y., Machleder, E. M., Chenchik, A., Li, R., and Siebert, P. D., Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques, 30, 892-897 (2001).
7. van Enckevort, F. H., Pepels, P. P., Leunissen, J. A., Martens, G. J., Wendelaar Bonga, S. E., and Balm, P. H., Oreochromis mossambicus (tilapia) corticotropin-releasing hormone: cDNA sequence and bioactivity. J. Neuroendocrinol., 12, 177-186 (2000).