A theoretical validation of the substrate depletion approach to determining kinetic parameters

Drug Metabolism and Disposition

Volumn 34, Issue 9, 2006, Pages 1433-1435

  Nath, Abhinav ^a   Atkins, William M ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450;

ARTICLE; DEPLETION; ENZYME MECHANISM; ENZYME SUBSTRATE; KINETICS; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; SIMULATION; THEORETICAL STUDY; VALIDATION PROCESS;

COMPUTER SIMULATION; CYTOCHROME P-450 ENZYME SYSTEM; KINETICS; METHODS; MODELS, THEORETICAL; PHARMACEUTICAL PREPARATIONS; REPRODUCIBILITY OF RESULTS;

EID: 33747838502     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.106.010777     Document Type: Article

References (6)

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3. Komura H and Iwaki M (2005) Nonlinear pharmacokinetics of propafenone in rats and humans: application of a substrate depletion assay using hepatocytes for assessment of nonlinearity. Drug Metab Dispos 33:726-732.
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5. Obach RS and Reed-Hagen AE (2002) Measurement of Michaelis constants for cytochrome P450-mediated biotransformation reactions using a substrate depletion approach. Drug Metab Dispos 30:831-837.
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