Optimization and high-level expression of a functional GST-tagged rHLT-B in Escherichia coli and GM1 binding ability of purified rHLT-B

Journal of Microbiology

Volumn 44, Issue 3, 2006, Pages 293-300

  Ma, Xingyuan ^a   Zheng, Wenyun ^a,b   Wang, Tianwen ^a   Wei, Dongzhi ^a   Ma, Yushu ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b SHENYANG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Expression optimization; GM1 binding ability; Lactose induction; rHLT B purification

Indexed keywords

ESCHERICHIA COLI;

BACTERIAL TOXIN; ENTEROTOXIN; ENTEROTOXIN LT; ESCHERICHIA COLI PROTEIN; GANGLIOSIDE GM1; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; ISOPROPYL THIOGALACTOSIDE; LACTOSE;

ARTICLE; BIOTECHNOLOGY; CHEMISTRY; CULTURE MEDIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EVALUATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY;

BACTERIAL TOXINS; BIOTECHNOLOGY; CULTURE MEDIA; ENTEROTOXINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; G(M1) GANGLIOSIDE; GLUTATHIONE TRANSFERASE; ISOPROPYL THIOGALACTOSIDE; LACTOSE; RECOMBINANT FUSION PROTEINS;

EID: 33747834066     PISSN: 12258873     EISSN: 12258873     Source Type: Journal    
DOI: None     Document Type: Article

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