Real-time assay of immobilized tannase with a stopped-flow conductometric device

Bioelectrochemistry

Volumn 69, Issue 1, 2006, Pages 113-116

  Chang, Fu Shiang ^a   Chen, Po Chung ^a   Chen, Richie L C ^a   Lu, Fu Ming ^a   Cheng, Tzong Jih ^a,b  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

Author keywords

Conductometric; Enzyme kinetics; Stopped flow; Tannic acid

Indexed keywords

BIOASSAY; BIOREACTORS; ENZYME KINETICS; MICROELECTRODES;

CONDUCTOMETRIC DEVICES; CONDUCTOMETRIC MEASUREMENT; ENZYME REACTOR; TANNIC ACID;

ENZYME IMMOBILIZATION;

BUFFER; GALLIC ACID METHYL ESTER; GALLIC ACID PROPYL ESTER; TANNIN;

ARTICLE; CONDUCTANCE; DEVICE; ELECTRODE; ENZYME REACTOR; KINETICS; MEASUREMENT; NONHUMAN; PH; SENSITIVITY ANALYSIS;

CARBOXYLIC ESTER HYDROLASES; ELECTRIC CONDUCTIVITY; ENZYME ACTIVATION; ENZYMES, IMMOBILIZED; FLOW INJECTION ANALYSIS; GALLIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; SENSITIVITY AND SPECIFICITY; TANNINS; TIME FACTORS;

EID: 33747827520     PISSN: 15675394     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioelechem.2005.12.004     Document Type: Article

References (11)

1. Bhat T.K., Singh B., and Sharma O.P. Microbial degradation of tannins - a current perspective. Biodegradation 9 (1998) 343-357
2. Lekha P.K., and Lonsane B.K. Production and application of tannin acyl hydrolase: state of the art. Adv. Appl. Microbiol. 44 (1997) 215-260
3. Mesing R. Immobilized Enzymes for Industrial Reactors (1975), Academic Press, New York
4. Haslam E., and Tanner R.J.N. Spectrophotometric assay of tannase. Phytochemistry 9 (1970) 2305-2309
5. Mondal K.C., Banerjee D., Jana M., and Pati B.R. Colorimetric assay method for determination of the tannin acyl hydrolase (EC 3.1.1.20) activity. Anal. Biochem. 295 (2001) 168-171
6. Inoue K.H., and Hagerman A.E. Determination of gallotannin with rhodanine. Anal. Biochem. 169 (1988) 363-369
7. Sharma S., Bhat T.K., and Dawra R.K. A spectrophotometric method for assay of tannase using rhodanine. Anal. Biochem. 279 (2000) 85-89
8. Chen R.L.C., and Matsumoto K. Enzymatic determination of gallotannin in green tea infusions by a photometric flow-injection analytical method. Anal. Sci. 11 (1995) 777-780
9. Weetall H.H., and Detar C.C. Immobilized tannase. Biotechnol. Bioeng. 16 (1974) 1095-1102
10. Horie H., Mukai T., Kohata K., and Goto T. Flow injection analysis of (-)epigallocatechin gallate in green tea infusions with an immobilized tannase reactor. Food Sci. Technol. Int. Tokyo 3 (1997) 27-30
11. Ramanathan K., and Danielsson B. Principles and applications of thermal biosensors. Biosens. Bioelectron. 16 (2001) 417-423