Novel lysine biosynthetic gene sequences (LYS1 and LYS5) used as PCR targets for the detection of the pathogenic Candida yeast

Applied Microbiology and Biotechnology

Volumn 72, Issue 2, 2006, Pages 416-420

  Guo, Shujuan ^a   Bhattacharjee, J K ^a  

^a MIAMI UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALBICANS CELLS; POLYMERASE CHAIN REACTION (PCR); RDNA CONTROL PRIMERS; SACCHAROPINE DEHYDROGENASE (SDH);

CONTAMINATION; DNA; FUNGI; GENES; MICROORGANISMS; YEAST;

BIOASSAY;

LYSINE; PANTETHEINE PHOSPHATE; PHOSPHOPANTETHEINYL TRANSFERASE; SACCHAROPINE DEHYDROGENASE; TRANSFERASE; UNCLASSIFIED DRUG;

BIOASSAY; DNA; GENETIC ANALYSIS; PATHOGEN; POLYMERASE CHAIN REACTION; YEAST;

ARTICLE; BIOSYNTHESIS; CANDIDA ALBICANS; CANDIDA KEFYR; CANDIDA LUSITANIAE; CANDIDA TROPICALIS; CANDIDIASIS; DNA ISOLATION; EARLY DIAGNOSIS; FUNGAL DETECTION; FUNGAL GENE; GENE AMPLIFICATION; GENE SEQUENCE; GENE TARGETING; LYS1 GENE; LYS5 GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CANDIDA; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; LYSINE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SACCHAROPINE DEHYDROGENASES; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

BACTERIA (MICROORGANISMS); CANDIDA; CANDIDA ALBICANS; CANDIDA TROPICALIS; CLAVISPORA LUSITANIAE; KLUYVEROMYCES MARXIANUS;

EID: 33747718705     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0470-y     Document Type: Article

References (22)

1. Ahmad S, Mustafa AS, Khan Z, Al-Rifaiy AI, Khan ZU (2004) PCR-enzyme immunoassay of rDNA in the diagnosis of candidemia and comparison with amplicon detection by agarose gel electrophoresis. Int J Med Microbiol 294:45-51
2. Arancia S, Sandini S, Cassone A, De Bernardis F, La Valle R (2004) Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C. albicans. Mol Cell Probes 18:171-175
3. Ausubel FM (1998) Current protocols in molecular biology. Wiley, New York, New York, p 13.11.1
4. Bhattacharjee JK (1992) Evolution of α-aminoadipate pathway for the synthesis of lysine in fungi. In: Mortlock RP (ed) Evolution of metabolic function. CRC, Boca Raton, Florida, pp 47-80
5. Bhattacharjee JK, Suvarna K, Bhattacherjee V (2002) Reagents and kits for detecting fungal pathogens in biological sample. U.S. Patent No. 6455248 B1
6. Broquist HP (1971) Lysine biosynthesis in yeast. Methods Enzymol 17B:112-129
7. Burgener-Kairuz P, Zuber JP, Jaunin P, Buchman TG, Bille J, Rossier M (1994) Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment. J Clin Microbiol 32:1902-1907
8. Crampin AC, Matthews RC (1993) Application of the polymerase chain reaction to the diagnosis of candidosis by amplification of an HSP 90 gene fragment. J Med Microbiol 39:233-238
9. Ehmann DE, Gehring AM, Walsh CT (1999) Lysine biosynthesis in Saccharomyces cerevisia: mechanism of α-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5. Biochem 38:6171-6177
10. Garrad RC, Bhattacharjee JK (1992) Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans. J Bacteriol 174:7379-7384
11. Garrad RC, Schmidt TM, Bhattacharjee JK (1994) Molecular and functional analysis of the LYS1 gene of Candida albicans. Infect Immun 62:5027-5031
12. Guo S, Bhattacharjee JK (2003) Molecular characterization of the Candida albicansLYS5 gene and site-directed mutational analysis of the PPTase (Lys5p) domains for lysine biosynthesis. FEMS Microbiol Lett 224:261-267
13. Guo S, Bhattacharjee JK (2004) Posttranslational activation, site-directed mutation and phylogenetic analyses of the lysine biosynthesis enzymes alpha-aminoadipate reductase Lys1p (AAR) and the phosphopantetheinyl transferase Lys7p (PPTase) from Schizosaccharomyces pombe. Yeast 21:1279-1288
14. Guo S, Evans SA, Wilkes MB, Bhattacharjee JK (2001) Novel posttranslational activation of the LYS2-encoded alpha-aminoadipate reductase for biosynthesis of lysine and site-directed mutational analysis of conserved amino acid residues in the activation domain of Candida albicans. J Bacteriol 183:7120-7125
15. Guo S, Garrad RC, Bhattacharjee JK (2006) Functional analysis through site-directed mutations and phylogeny of the Candida albicansLYS1-encoded saccharopine dehydrogenase. Mol Genet Genomics (MGG) 275:74-80
16. Hijarrubia MJ, Aparicio JF, Casqueiro J, Martin JF (2001) Characterization of the lys2 gene of Acremonium chrysogenum encoding a functional α-aminoadipate activating and reducing enzyme. Mol Gen Genet (MGG) 264:755-762
17. Kan VL (1993) Polymerase chain reaction for the diagnosis of candidemia. J Infect Dis 168:779-783
18. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acid Res 30:3059-3066
19. Miyakawa Y, Mabuchi T (1994) Characterization of a species-specific DNA fragment originating from the Candida albicans mitochondrial genome. J Med Vet Mycol 32:71-75
20. Pfaller MA, Diekema DJ (2002) Role of sentinel surveillance of candidemia: trends in species distribution and antifungal susceptibility. J Clin Microbiol 40:3551-3557
21. Reiss E, Obayashi T, Orle K, Yoshida M, Zancope-Oliveira RM (2000) Non-culture based diagnostic tests for mycotic infections. Med Mycol 38(Suppl 1):147-159
22. van Burik JA, Magee PT (2001) Aspects of fungal pathogenesis in humans. Annu Rev Microbiol 55:743-772