Heterologous expression of the benzoate para-hydroxylase encoding gene (CYP53B1) from Rhodotorula minuta by Yarrowia lipolytica

Applied Microbiology and Biotechnology

Volumn 72, Issue 2, 2006, Pages 323-329

  Shiningavamwe, Andreas ^a   Obiero, George ^a   Albertyn, Jacobus ^a   Nicaud, Jean Marc ^b   Smit, Martie ^a  

^a UNIVERSITY OF THE FREE STATE   (South Africa)

^b AGROPARISTECH   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME; HYDROXYLASE ACTIVITY; MONOOXYGENASE; REDUCTASE;

APPROXIMATION THEORY; BACTERIA; CELL CULTURE; ENZYMES; HYDROXYLATION; PH EFFECTS; YEAST;

GENES;

4 HYDROXYBENZOIC ACID; BENZOATE 4 MONOOXYGENASE; BENZOIC ACID DERIVATIVE; COMPLEMENTARY DNA; CYTOCHROME P450; ISOCITRATE LYASE; OXYGENASE; STEARIC ACID; UNCLASSIFIED DRUG;

BIOTRANSFORMATION; CYTOCHROME; DNA; ENZYME ACTIVITY; GENE EXPRESSION; RECOMBINATION; YEAST;

ARTICLE; BIOTRANSFORMATION; CPR GENE; CYP53B1 GENE; ENZYME ACTIVITY; FUNGAL GENE; FUNGAL STRAIN; GENE OVEREXPRESSION; HYDROXYLATION; MOLECULAR CLONING; NONHUMAN; POX2 GENE; PROMOTER REGION; RHODOTORULA; RHODOTORULA MINUTA; WHOLE CELL; YARROWIA LIPOLYTICA;

BENZOATE 4-MONOOXYGENASE; BENZOIC ACID; BIOTRANSFORMATION; BLOTTING, SOUTHERN; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHODOTORULA; RNA, MESSENGER; TRANSFORMATION, GENETIC; YARROWIA;

RHODOTORULA MINUTA; YARROWIA LIPOLYTICA;

EID: 33747714615     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0264-7     Document Type: Article

References (17)

1. Backes WL, Kelley RW (2003) Organization of multiple cytochrome P450s with NADPH-cytochrome P450 reductase in membranes. Rev Pharmacol Ther 98:221-233
2. Barth G, Gaillardin C (1996) Yarrowia lipolytica. In: Wolf K (ed) Nonconventional yeasts in biotechnology, a handbook. Springer, Berlin Heidelberg, New York, pp 313-388
3. Dong J, Porter TD (1996) Coexpression of mammalian P450 and reductase in Escherichia coli. Arch Biochem Biophys 327:254-259
4. Duetz WA, Van Beilen BJ, Witholt B (2001) Using proteins in their natural environment: potential and limitations of microbial whole cell hydroxylations in applied biocatalysis. Curr Opin Biotechnol 12:419-425
5. Fickers P, Benetti P-H, Waché Y, Marty A, Mauersberger S, Smit MS, Nicaud J-M (2005) Hydrophobic substrate utilization by the yeast Yarrowia lipolytica and its potential applications. FEMS Yeast Res 5:527-543
6. Fujii T, Ogawa T, Fukuda H (1987) Isobutene production by Rhodotorula minuta. Appl Microbiol Biotechnol 25:430-433
7. Fujii T, Nakamura K, Shibuya K, Tanase S, Gotoh O, Ogawa T, Fukuda H (1997) Structural characterization of the gene and corresponding cDNA for the cytochrome P450rm from Rhodotorula minuta which catalyzes formation of isobutene and 4-hydroxylation of benzoate. Mol Gen Genet 256:115-120
8. Iida T, Sumita T, Ohta A, Takagi M (2000) The cytochrome P450ALK multigene family of an n-alkane-assimilating yeast, Yarrowia lipolytica: cloning and characterization of genes coding for new CYP52 family members. Yeast 16:1077-1087
9. Juretzek T, Mauersberger S, Barth G (2000a) Recombinant haploid or diploid Yarrowia lipolytica cells for the functional heterologous expression of cytochrome P450 systems. World Patent Apllication WO0003008
10. Juretzek T, Wang HJ, Nicaud J-M, Mauersberger S, Barth G (2000b) Comparison of promoters suitable for regulated overexpression of β-galactosidase in the alkane-utilizing yeast Yarrowia lipolytica. Biotechnol Bioprocess Eng 5:320-326
11. Juretzek T, Le Dall M, Mauersberger S, Gaillardin C, Barth G, Nicaud J-M (2001) Vectors for gene expression and amplification in the yeast Yarrowia lipolytica. Yeast 18:97-113
12. Liu Y, Kondo A, Ohkawa H, Shiota N, Fukuda H (1998) Bioconversion using immobilized recombinant flocculent yeast cells carrying a fused enzyme gene in an 'intelligent' bioreactor. Biochem Eng J 2:229-235
13. Mlickova K, Roux E, Athenstaedt K, Andrea S, Daum G, Chardot T, Nicaud J-M (2004) Involvement of Aox2p, the major acyl CoA oxidase of the yeast Yarrowia lipolytica, in lipid accumulation and formation of lipid bodies. Appl Environ Microbiol 70:3918-3924
14. Nthangeni MB, Urban P, Pompon D, Smit MS, Nicaud J-M (2004) The use of Yarrowia lipolytica for the expression of human cytochrome P450 CYP1A1. Yeast 21:583-592
15. Pignède G,Wang JH, Fudalej H, Seman M, Gaillardin C, Nicaud J-M (2000) Autocloning and amplification of LIP2 in Yarrowia lipolytica. Appl Environ Microbiol 66:3283-3289
16. Sambrook J, Russel D (2001) Molecular cloning. A laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
17. Werck-Reichhart WD, Feyereinsen R (2000) Cytochromes P450: a success story. Genome Biol 1:3003.1-3003.9