Cloning and expression of cellulase XF-818 of xylella fastidiosa in escherichia coli;Clonagem e expressão da celulase XF-818 de xylella fastidiosa em escherichia coli

Scientia Agricola

Volumn 60, Issue 4, 2003, Pages 715-721

  Wulff, Nelson Arno ^a,b   Carrer, Helaine ^a   Pascholati, Sérgio Florentino ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b Fundo de Defesa da Citricultura   (Brazil)

Author keywords

Cellulases; Citrus variegated chlorosis; Cloning and expresssion

Indexed keywords

EID: 33747614359     PISSN: 01039016     EISSN: 1678992X     Source Type: Journal    
DOI: 10.1590/S0103-90162003000400016     Document Type: Article

References (41)

1. ALTSCHUL, S.F.; GISH, W.; MILLER, W.; MYERS, E.W.; LIPMAN, D.J. Basic local alignment search tool. Journal of Molecular Biology, v.215, p.403-410, 1990.
2. BHATTACHARYYA, A.; STILWAGEN, S.; IVANOVA, N. et al., Whole-genome comparative analysis of three phytopathogenic Xylella fastidiosa strains. Proceeding of the National Academy of Sciences of USA, v.99, p.12403-12408, 2002.
3. BRADFORD, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Analytical Biochemistry, v.72, p.248-254, 1976.
4. CHANG, C.J.; GARNIER, M.; ZREIK, L.; ROSSETTI, V.; BOVÉ, J.M. Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain of Xylella fastidiosa. Current Microbiology, v.27, p.137-142, 1993.
5. DOW, J.M.; DANIELS, M.J. Xylella fastidiosa genomics and bacterial pathogenicity to plants. Yeast, v.17, p.263-271, 2000.
6. FOONG, F.C.-F.; DOI, R.H. Characterization and comparison of Clostridium cellulovorans endoglucanases-xylanases EngB and EngD hyperexpressed in Escherichia coli. Journal of Bacteriology, v.174, p.1403-1409, 1992.
7. FRY, S.M.; HUANG, J.S.; MILHOLLAND, R.D. Isolation and preliminary characterization of extracellular proteases produced by strains of Xylella fastidiosa from grapevines. Phytopathology, v.84, p.357-363, 1994.
8. GORDON, D.; ABAJIAN, C.; GREEEN, P. Consed: a graphical tool for sequence finishing. Genome Research, v.8, p.195-202, 1998.
9. GOUGH, C.L.; DOW, J.M.; BARBER, C.E.; DANIELS, M.J. Cloning of two endoglucanases genes of Xanthomonas campestris pv. campestris: analysis of the role of the major endoglucanase in pathogenicity. Molecular Plant Microbe Interactions, v.1, p.275-281, 1988.
10. GOUGH, C.L.; DOW, J.M.; KEEN, J.; HENRISSAT, B.; DANIELS, M.J. Nucleotide sequence of the engXCA gene encoding the major endoglucanase of Xanthomonas campestris pv.campestris. Gene, v.89, p.53-59, 1990.
11. HENRISSAT, B. A classification of glycosyl hydrolases based on amino-acid sequence similarities.Biochemical Journal, v.280, p.309-316, 1991.
12. HENRISSAT, B.; BAIROCH, A. New families in the classification of glycosyl hydrolases based on amino-acid sequence similarities. Biochemical Journal, v.293, p.781-788, 1993.
13. HOPKINS, D.L. Physiological and pathological characteristics of virulent an avirulent strains of the bacterium that causes Pierce’s disease of grapevine. Phytopathology, v.75, p.713-717, 1985.
14. HOPKINS, D.L. Xylella fastidiosa: xylem-limited bacterial pathogen of plants. Annual Review of Phytopathology, v.27, p.271-290, 1989.
15. HOUGH, D.W.; DANSON, M.J. Extremozymes. Current Opinion i n Chemical Biology, v.3, p.39-46, 1999.
16. JAHR, H.; DREIER, J.; MELETZUS, D.; BAHRO, R.; EICHENLAUB, R. The endo-β-1,4-glucanase CelA of Clavibacter michiganensis subsp. michiganensis is a pathogenicity determinant required for induction of bacterial wilt of tomato. Molecular Plant Microbe Interactions, v.13, p.703-714, 2000.
17. LAEMMLI, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, v.227, p.680-685, 1970.
18. LAINE, M.J.; HAAPALAINEN, M.; WAHLROSS, T.; KANKARE, K.; NISSINEN, R.; KASSUWI, S.; METZLER, M.C. The cellulase encoded by the native plasmid of Clavibacter michiganensis ssp. sepedonicus plays a role in virulence and contains an expansin-like domain. Physiological and Molecular Plant Pathology, v.57, p.221-233, 2000.
19. LAMBAIS, M.R.; GOLDMAN, M.H.S.; CAMARGO, L.E.A.; GOLDMAN, G.H. A genomic approach to the understanding of Xylella fastidiosa pathogenicity. Current Opinion in Microbiology, v.3, p.459-462, 2000.
20. 2+ and thiols mediate the aggregation and adhesion of X. fastidiosa. Brazilian Journal of Medical and Biological Research, v.35, p.645-650, 2002.
21. LEVER, M. A new reaction for colorimetric determination of carbohydrates. Analytical Biochemistry, v.47, p.273-279, 1972.
22. LOPES, J.R.S. Estudos com vetores de Xylella fastidiosa e implicações no manejo da clorose variegada dos citros. Laranja, v.20, p.329-344, 1996.
23. MÄE, A.; HEIKINHEIMO, R.; PALVA, E.T. Structure and regulation of Erwinia carotovora subsp. carotovora SCC3193 Cellulase gene celV1 and the role of cellulase in phytopathogenicity. Molecular and General Genetics, v.247, p.17-26, 1995.
24. MONTEIRO, P.B.; TEIXEIRA, D.C.; P ALMA, R.R.; GARNIER, M.; BOVE, J.M.; RENAUDIN, J. Stable transformation of the Xylella fastidiosa citrus variegated chlorosis strain with oriC plasmids. Applied and Environmental Microbiology, v.67, p.2263-2269, 2001.
25. NIELSEN, H.; ENGELBRECHT, J.; BRUNAK, S.; VON HEIJNE, G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering, v.10, p.1-6, 1997.
26. NILSSON, J.; STAHL, S.; LUNDEBERG, J.; UHLÉN, M.; NYGREN, P.-A. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expression and Purification, v.11, p.1-16, 1997.
27. PARK, Y.W.; LIM, S.T.; YUN, H.D. Cloning and sequencing of the celA gene encoding CMCase of Erwinia carotovora subsp. carotovora LY34. Molecules and Cells, v.8, p.27-35, 1998.
28. PURCELL, A.H.; HOPKINS, D.L. Fastidious xylem-limited bacterial plant pathogens. Annual Review of Phytopathology, v.34, p.131-151, 1996.
29. ROBERTS, D.P.; DENNY, T.P.; SCHELL, M.A. Cloning of the egl gene of Pseudomonas sol anacearum and anal ysi s of i t s rol e i n phytopathogenicity. Journal of Bacteriology, v.170, p.1445-1451, 1988.
30. ROSSETTI, V.; DE NEGRI, D. Clorose variegada dos citros: revisão. Laranja, v.11, p.1-14, 1990.
31. ROSSETTI, V.; GARNIER, M.; BOVÉ, J.M.; BERETTA, M.J.G.; TEIXEIRA, A.R.R.; QUAGGIO, J.A.; DE NEGRI, J.D. Présence de bactéries dans le xylème d’oranges atteints de chlorose variégée, une nouvelle maladie des agrumes au Brésil. Comptus Rendus de Academie Sciences Paris, v.310, p.345-349, 1990.
32. ROSSETTI, V.; CARVALHO, M.L.V.; CHAGAS, C.M. Ensaios de transmissão de clorose variegada dos citros (CVC) em campo. Fitopatologia Brasileira, v.20, p.67, 1995. Suplemento.
33. SAMBROCK, J.; FRITISCH, E.F.; MANIATIS, J. Molecular cloning. 2.ed. Plainview: Cold Spring Harbor Laboratory. Press, 1989.
34. SCARPARI, L.M. Modulação da expressão de genes de patogenicidade putativos em Xylella fastidiosa sob condições de baixa e alta densidade celular. Piracicaba: USP/ESALQ, 2001. 85p. (Dissertação - Mestrado)
35. SILVA, F.R. DA; VETORE, A.L.; EDSON, L.K.; LEITE, A.; ARRUDA, P. Fastidian gum: the Xylella fastidiosa exopolysaccharide possibly involved in bacterial pathogenicity. FEMS Microbiology Letters, v.203, p.165-171, 2001.
36. SILVA NETO, J.F.; KOIDE, T.; GOMES, S.L.; MARQUES, M.V. Site-directed gene disruption in Xylella fastidiosa. FEMS Microbiology Letters, v.210, p.105-110, 2002.
37. SIMPSON, A.J.G.; REINACH, F.C.; ARRUDA, P. et al. The genome sequence of the plant pathogen Xylella fastidiosa. Nature, v.406, p.151-159, 2000.
38. STUDIER, F.W.; MOFFAT T, B.A. Use of bacteriophage-T7 RNA-polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology, v.189, p.113-130, 1986.
39. TUBELIS, A.; BARROS, J.C.; LEITE, R.M.V.B. Difusão da clorose variegada dos citros em pomares comerciais de laranja doce no Brasil. Laranja, v.14, p.239-254, 1993.
40. VAN-SLUYS, M.A.; OLIVEIRA, M.C.; MONTEIRO-VITORELLO, C.B. et al., Comparative analyses of the complete genome sequences of Pierce’s disease and citrus variegated chlorosis strains of Xylella fastidiosa. Journal of Bacteriology, v.185, p.1018-1026, 2003.
41. WOOD, P.J.; ERFLE, J.D.; TEATHER, R.M. Use of complex formation between congo red and polysaccharide in detection and assay of polysaccharide hydrolases. Methods in Enzymology, 160, p.59-74, 1988.