Stability of transgene integration and expression in subsequent generations of doubled haploid oilseed rape transformed with chitinase and β-1,3-glucanase genes in a double-gene construct

Plant Cell Reports

Volumn 25, Issue 9, 2006, Pages 942-952

  Melander, Margareta ^a,c   Kamnert, Iréne ^a,c   Happstadius, Ingrid ^a   Liljeroth, Erland ^b   Bryngelsson, Tomas ^b  

^a Svalof Weibull AB   (Sweden)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^c Plant Science Sweden AB   (Sweden)

Author keywords

Brassica napus; Doubled haploids; Expression stability; Gene silencing; Transgene deletion

Indexed keywords

BRASSICA NAPUS; DOUBLED HAPLOIDS; EXPRESSION STABILITY; GENE SILENCING; TRANSGENE DELETION;

CROPS; ENZYMES; GENES; REAL TIME SYSTEMS;

GENETIC ENGINEERING;

CHITINASE; GLUCAN ENDO 1,3 BETA GLUCOSIDASE;

ARTICLE; BARLEY; DRUG EFFECT; FUNGUS; GENE EXPRESSION; GENETICS; HAPLOIDY; METABOLISM; MICROBIOLOGY; PLANT DISEASE; TRANSGENE; TRANSGENIC PLANT; TURNIP; WESTERN BLOTTING;

BLOTTING, WESTERN; BRASSICA RAPA; CHITINASE; FUNGI; GENE EXPRESSION; GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE; HAPLOIDY; HORDEUM; PLANT DISEASES; PLANTS, GENETICALLY MODIFIED; TRANSGENES;

ENZYMES; FARM CROPS; GENES; GENETIC ENGINEERING;

BRASSICA NAPUS; FUNGI; HORDEUM VULGARE SUBSP. VULGARE;

EID: 33747387169     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-006-0153-2     Document Type: Article

References (44)

1. Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H (1982) Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19:327-336
2. Becker D, Kemper E, Schell J, Masterson R (1992) New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol 20:1195-1197
3. Bieri S, Potrykus I, Fütterer J (2003) Effects of combined expression of antifungal barley seed proteins in transgenic wheat on powdery mildew infection. Mol Breed 11:37-48
4. Bliffeld M, Mundy J, Potrykus I, Fütterer J (1999) Genetic engineering of wheat for increased resistance to powdery mildew disease. Theor Appl Genet 98:1079-1086
5. Broglie K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais CJ, Broglie R (1991) Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254:1194-1197
6. Bryngelsson T, Greén B (1989) Characterization of a pathogenesis-related, thaumatin-like protein isolated from barley challenged with an incompatible race of mildew. Physiol Mol Plant Pathol 35:45-52
7. Bryngelsson T, Sommer-Knudsen J, Gregersen PL, Collinge DB, Ek B, Thordal-Christensen H (1994) Purification, characterization and molecular cloning of basic PR-1-type pathogenesis-related proteins from barley. Mol Plant Microbe Interact 7:267-275
8. Davies GJ, Sheikh MA, Ratcliffe OJ, Coupland G, Furner IJ (1997) Genetics of homology-dependent gene silencing in Arabidopsis; a role for methylation. Plant J 12:791-804
9. Dickson MH, Petzoldt R (1996) Breeding for resistance to Sclerotinia sclerotiorum in Brassica oleracea. Acta Hortic 407:103-108
10. Elmayan T, Vaucheret H (1996) Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally. Plant J 9:787-797
11. Fagard M, Vaucheret H (2000) (Trans)gene silencing in plants: how many mechanisms? Ann Rev Plant Physiol Plant Mol Biol 51:167-194
12. Fladung M (1999) Gene stability in transgenic aspen (Populus). I. Flanking DNA sequences and T-DNA structure. Mol Gen Genet 260:574-581
13. Grison R, Grezes-Besset B, Schneider M, Lucante N, Olsen L, Legnay J-J, Toppan A (1996) Field tolerance to fungal pathogens of Brassica napus constitutively expressing a chimeric chitinase gene. Nat Biotechnol 14:643-646
14. Hernández M, Rio A, Esteve T, Prat S, Pla M (2001) A rapeseed-specific gene, acetyl-CoA carboxylase, can be used as a reference for qualitative and real-time quantitative PCR detection of transgenes from mixed food samples. J Agric Food Chem 49:3622-3627
15. Howie W, Joe L, Newbigin E, Suslow T, Dunsmuir P (1994) Transgenic tobacco plants which express the chiA gene from Serratia marcescens have enhanced tolerance to Rhizoctonia solani. Transgenic Res 3:90-98
16. Jach G, Görnhardt B, Mundy J, Logemann J, Pinsdorf E, Leah R, Schell J, Maas C (1995) Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J 8:97-109
17. Jongedijk E, Tigelaar H, van Roekel JSC, Bres-Vloemans SA, Dekker I, van den Elzen PJM, Cornelissen BJC, Melchers LS (1995) Synergistic activity of chitinase and β-1,3-glucanase enhances fungal resistance in transgenic tomato plants. Euphytica 85:173-180
18. Kølster P, Munk L, Stølen O, Løhde J (1986) Near-isogenic barley lines with genes for resistance to powdery mildew. Crop Sci 26:903-907
19. Kumpatla SP, Teng W, Buchholz WG, Hall TC (1997) Epigenetic transcriptional silencing and 5-azacytidine-mediated reactivation of a complex transgene in rice. Plant Physiol 115:361-373
20. Kunz C, Schöb H, Leubner-Metzger G, Glazov E, Meins FJ (2001) β-1,3-glucanase and chitinase transgenes in hybrids show distinctive and independent patterns of posttranscriptional gene silencing. Planta 212:234-249
21. Leah R, Tommerup H, Svendsen I, Mundy J (1991) Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J Biol Chem 266:1564-1573
22. Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Zeitschrift für Pflanzenphysiologie 105:427-434
23. Lusso M, Kuc J (1996) The effect of sense and antisense expression of the PR-N gene for β-1,3-glucanase on disease resistance of tobacco to fungi and viruses. Physiol Mol Plant Pathol 49:267-283
24. Marsh JL, Erfle M, Wykes EJ (1984) The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481-485
25. Mengistu A, Rimmer SR, Koch E, Williams PH (1991) Pathogenicity grouping of isolates of Leptosphaeria maculans on Brassica napus cultivars and their disease reaction profiles on rapid-cycling brassicas. Plant Dis 75:1279-1282
26. Moloney MM, Walker JM, Sharma KK (1989) High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep 8:238-242
27. Mora AA, Earle ED (2001) Resistance to Alternaria brassicicola in transgenic broccoli expressing a Thrichoderma harzianum endochitinase gene. Mol Breeding 8:1-9
28. Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279-289
29. Neuhaus J-M, Ahl-Goy P, Hinz U, Flores S, Meins F (1991) High-level expression of a tobacco chitinase gene in Nicotiana sylvestris. Susceptibility of transgenic plants to Cercospora nicotianae infection. Plant Mol Biol 16:141-151
30. Oldach KH, Becker D, Lörz H (2001) Heterologous expression of genes mediating enhanced fungal resistance in transgenic wheat. Mol Plant Microbe Interact 14:832-838
31. Park Y-D, Papp I, Moscone EA, Iglesias VA, Vaucheret H, Matzke AJM, Matzke MA (1996) Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity. Plant J 9:183-194
32. Plümper B, Sacristan MD (1994) Resistance to Alternaria brassicae in n = 11 Brassica species and its transfer to Brassica napus. In: Abstracts of the ISHS Symposium on Brassicas/Ninth Crucifer Genetics Workshop, Lisbon, Portugal, 15-19 November 1994
33. Richards E, Reichardt M, Rogers S (1994-2000) Preparation of genomic DNA from plant tissue. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology, vol 1. Wiley, New York, pp 2.3.1-2.3.7
34. Risseeuw E, Franke-van Dijk MEI, Hooykaas PJJ (1997) Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome. Plant J 11:717-728
35. Sjödin C, Glimelius K (1988) Screening for resistance to blackleg Phoma lingam (Tode ex Fr.) Desm. within Brassicaceae. J Phytopathol 123:322-332
36. Steventon LA, Happstadius I, Okori P, Dixelius C (2002) Development of a rapid technique for the evaluation of the response of Brassica napus to Verticillium wilt. Plant Dis 86:854-858
37. Tandrup Poulsen T, Bryngelsson T, Greén B, Troedsson U, Collinge DB, Thordal-Christensen H (in preparation) The pathogenesis-related (PR) proteins of barley leaves inhibit the growth of barley powdery mildew conidia in vitro
38. Tang J, Scarth R, McVetty PBE (2004) Stability of the expression of acyl-ACP thioesterase transgenes in oilseed rape doubled haploid lines. Crop Sci 44:732-740
39. van den Elzen PJM, Jongedijk E, Melchers LS, Cornelissen BJC (1993) Virus and fungal resistance: from laboratory to field. Philos Trans R Soc 342:271-278
40. van der Krol AR, Mur LA, Beld M, Mol JNM, Stuitje AR (1990) Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2:291-299
41. Vaucheret H, Fagard M (2001) Transcriptional gene silencing in plants: targets, inducers and regulators. Trends Genet 17:29-35
42. Walkerpeach CR, Velten J (1994) Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual, 2nd edn. Kluwer, Dordrecht, pp B1:1-19
43. Yoshikawa M, Tsuda M, Takeuchi Y (1993) Resistance to fungal diseases in transgenic tobacco plants expressing the phytoalexin elicitor-releasing factor, β-1,3-endoglucanase, from soybean. Naturwissenschaften 80:417-420
44. Zhu Q, Maher EA, Masoud S, Dixon RA, Lamb CJ (1994) Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase genes in transgenic tobacco. Bio/Technol 12:807-812