A novel real-time system to monitor cell aggregation and trajectories in rotating wall vessel bioreactors

Journal of Biotechnology

Volumn 125, Issue 3, 2006, Pages 416-424

  Manley, Prakash ^a   Lelkes, Peter I ^a  

^a DREXEL UNIVERSITY   (United States)

Author keywords

aggregation; CCD; HepG2 cells; Micro gravity; PC12 cells; Real time monitoring; RWV bioreactor

Indexed keywords

HEPG2 CELLS; MICRO-GRAVITY; PC12 CELLS; RWV BIOREACTOR;

ALGORITHMS; CELL CULTURE; CONTROL SYSTEMS; LASER BEAMS; LIGHT EMISSION; REACTION KINETICS; REAL TIME SYSTEMS; TISSUE;

BIOREACTORS;

FLUORESCENT DYE; MICROSPHERE;

ANIMAL CELL; ARTICLE; BIOREACTOR; CAMERA; CELL AGGREGATION; CELL LABELING; CELL SIZE; CELL SUSPENSION; CELL TYPE; IMAGING SYSTEM; IRRADIATION; LASER; NONHUMAN; PRIORITY JOURNAL; RAT; SPHEROID CELL; VALIDATION PROCESS;

ANIMALS; BIOREACTORS; CALIBRATION; CELL AGGREGATION; CELL COUNT; COMPUTER SYSTEMS; FLUORESCENT DYES; HUMANS; MICROSPHERES; PARTICLE SIZE; PC12 CELLS; RATS; ROTATION; STATISTICAL DISTRIBUTIONS; TIME FACTORS;

EID: 33747127426     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.03.030     Document Type: Article

References (19)

1. Cowger N.L., O'Connor K.C., Hammond T.G., Lacks D.J., and Navar G.L. Characterization of bimodal cell death of insect cells in a rotating-wall vessel and shaker flask. Biotechnol. Bioeng. 64 1 (1999) 14-26
2. Duke P.J., Daane E.L., and Montufar-Solis D. Studies of chondrogenesis in rotating systems. J. Cell. Biochem. 51 3 (1993) 274-282 (review)
3. Duke P.J., Daane E.L., Arizpe J., and Montufar-Solis D. Chondrogenesis in aggregates of embryonic limb cells grown in a rotating wall vessel. Adv. Space Res. 17 6/7 (1996) 289-293
4. Enmon Jr. R.M., O'Connor K.C., Song H., Lacks D.J., and Schwartz D.K. Aggregation kinetics of well and poorly differentiated human prostate cancer cells. Biotechnol. Bioeng. 80 5 (2002) 580-588
5. Fujita K., Lazarovici P., and Guroff G. Regulation of the differentiation of PC12 pheochromocytoma cells. Environ. Health Perspect. 80 (1989) 127-142 (review)
6. Gao H., Ayyaswamy P.S., and Ducheyne P. Dynamics of a microcarrier particle in the simulated microgravity environment of a rotating-wall vessel. Microgravity Sci. Technol. 10 3 (1997) 154-165
7. Goodwin T.J., Schroeder W.F., Wolf D.A., and Moyer M.P. Rotating-wall vessel co-culture of small intestine as a prelude to tissue modeling: aspects of simulated microgravity. Proc. Soc. Exp. Biol. Med. 202 2 (1993) 181-192
8. Khaoustov V.I., Risin D., Pellis N.R., and Yoffe B. Microarray analysis of genes differentially expressed in HepG2 cells cultured in simulated microgravity: preliminary report. In Vitro Cell Dev. Biol. Anim. 37 2 (2001) 84-88
9. Lelkes P.I., and Miller I.R. Perturbations of membrane structure by optical probes. I. Location and structural sensitivity of merocyanine 540 bound to phospholipid membranes. J. Membr. Biol. 52 1 (1980) 1-15
10. Lelkes P.I., Unsworth B.R., Saporta S., Cameron D.F., and Gallo G. Culture of neuroendocrine and neuronal cells for tissue engineering. In: Vunjak-Novakovic G., and Freshney R.I. (Eds). Culture of Cells for Tissue Engineering (2004), Wiley-Liss Inc., New York, NY
11. Muhitch J.W., O'Connor K.C., Blake D.A., Lacks D.J., Rosenzweig N., and Spaulding G.F. Characterization of aggregation and protein expression of bovine corneal endothelial cells as microcarrier cultures in a rotating-wall vessel. Cytotechnology 32 (2000) 253-263
12. Nguyen P.D., and O'Rear E.A. Temporal aggregate size distributions from simulation of platelet aggregation and disaggregation. Ann. Biomed. Eng. 18 4 (1990) 427-444
13. Nickerson C.A., Ott M.C., Wilson J.W., Ramamurthy R., and Pierson D.L. Microbial responses to microgravity and other low-shear environments. Microbiol. Mol. Biol. Rev. 68 2 (2004) 345-361 (review)
14. Pollack S.R., Meaney D.F., Levine E.M., Litt M., and Johnston E.D. Numerical model and experimental validation of microcarrier motion in a rotating bioreactor. Tiss. Eng. 6 5 (2000) 519-530
15. Qiu Q.Q., Ducheyne P., and Ayyaswamy P.S. Fabrication, characterization and evaluation of bioceramic hollow microspheres used as microcarriers for 3D bone tissue formation in rotating bioreactors. Biomaterials 20 11 (1999) 989-1001
16. Schwarz R.P., Goodwin T.J., and Wolf D.A. Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity. J. Tiss. Cult. Meth. 14 (1992) 51-58
17. Smoluchowski M.V. Versuch einer mathematischen theorie der koagulationskinetik kolloider loesungen. Z. Phys. Chem. 92 (1917) 129-168
18. Unsworth B.R., and Lelkes P.I. Growing tissues in microgravity. Nat. Med. 4 8 (1998) 901-907
19. Vidulescu C., Clejan S., and O'Connor K.C. Vesicle traffic through intercellular bridges in DU 145 human prostate cancer cells. J. Cell. Mol. Med. 8 3 (2004) 388-396