High-level expression of recombinant human paraoxonase 1 Q in silkworm larvae (Bombyx mori)

Applied Microbiology and Biotechnology

Volumn 72, Issue 1, 2006, Pages 103-108

  Zhu, Jie ^a   Ze, Yuguan ^a   Zhang, Chi ^a   Zang, Yuhui ^a   Lu, Haiqin ^a   Chu, Ping ^a   Sun, Manji ^b   Qin, Junchuan ^a  

^a NANJING UNIVERSITY   (China)

^b BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BOMBYX MORI; CALCIUM-DEPENDENT PARAOXONASE; RECOMBINANT HUMAN PARAOXONASE; SILKWORM LARVAE;

CALCIUM; CHOLESTEROL; ENZYME KINETICS; INSECTICIDES; MOLECULAR WEIGHT; PROTEINS;

BIOLOGICAL ORGANS;

2 DIETHYLAMINOETHANOL; APOLIPOPROTEIN A1; ARYLDIALKYLPHOSPHATASE; ARYLESTERASE; ESTER DERIVATIVE; GLUCONOLACTONASE; HIGH DENSITY LIPOPROTEIN; LACTONE DERIVATIVE; NERVE GAS; ORGANOPHOSPHATE INSECTICIDE; PARAOXONASE 1 Q; RECOMBINANT ENZYME; SEPHAROSE; UNCLASSIFIED DRUG; VIRUS VECTOR;

CHROMATOGRAPHY; ELECTROKINESIS; ENZYME; GENE EXPRESSION; LARVA; MOTH; RECOMBINATION;

ANIMAL CELL; ARTICLE; ATHEROSCLEROSIS; BACULOVIRUS; CHOLESTEROL TRANSPORT; ENZYME MECHANISM; ENZYME PURIFICATION; HEMOLYMPH; HYDROLYSIS; MACROPHAGE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN FAMILY; SILKWORM; WESTERN BLOTTING;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ARYLDIALKYLPHOSPHATASE; BACULOVIRIDAE; BLOTTING, WESTERN; BOMBYX; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; HEMOLYMPH; HUMANS; MOLECULAR WEIGHT; RECOMBINANT PROTEINS;

BOMBYX MORI;

EID: 33747116220     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0246-9     Document Type: Article

References (21)

1. Aharoni A, Gaidukov L, Yagur S, Toker L, Silman I, Tawfik DS (2004) Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization. Proc Natl Acad Sci U S A 101(2):482-487
2. Broomfield CA, Ford KW (1991) Hydrolysis of nerve gases by plasma enzymes. In: Proceedings of the 3rd international meeting on cholinesterases, La Grande-Motte, France, p 161
3. Brushia RJ, Forte TM, Oda MN, La Du BN, Bielicki JK (2001) Baculovirus-mediated expression and purification of human serum paraoxonase 1A. J Lipid Res 42:951-958
4. Draganov DI, La Du BN (2004) Pharmacogenetics of paraoxonases: a brief review. Naunyn Schmiedebergs Arch Pharmacol 369:78-88
5. Draganov DI, Teiber JF, Speelman A, Osawa Y, Sunahara R, La Du BN (2005) Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. J Lipid Res 46:1239-1247
6. Durrington PN, Mackness B, Mackness MI (2001) Paraoxonases and atherosclerosis. Arterioscler Thromb Vasc Biol 21:473-480
7. Gaidukov L, Tawfik DS (2005) High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I. Biochemistry 44:11843-11854
8. Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RBG, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS (2004) Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzyme. Nat Struct Mol Biol 11:412-419
9. Harley RD, Billecke S, La Du BN (1999) Association of low PON1 type Q (type A) arylesterase activity with neurologic symptom complexes in Gulf War veterans. Toxicol Appl Pharmacol 157:233-239
10. Imai Y, Morita H, Kurihara H, Sugiyama T, Kato N, Ebihara A, Hamada C, Kurihara Y, Shindo T, Oh-hashi Y, Yazaki Y (2000) Evidence for association between paraoxonase gene polymorphisms and atherosclerotic diseases. Atherosclerosis 149:435-442
11. Khersonsky O, Tawfik DS (2005) Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase. Biochemistry 44:6371-6382
12. La Du BN, Kalow W (1992) Human serum paraoxonase/arylesterase. Genetic factors influencing the metabolism of foreign compounds. International encyclopedia of pharmacology and therapeutics. Pergamon, New York, pp 51-91
13. La Du BN, Adkins S, Chung-Liang AK, Lipsig D (1993) Studies on human serum paraoxonase/arylesterase. Chem Biol Interact 87:25-34
14. Peng R, Yu Z, Zang Y, Qin J (1999) High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm. Biotechnol Lett 21:979-985
15. Qiu P, Ding Y, Qin J, Han K, Zhu D (1994) Expression of biologically active monomeric form of human M-CSF in baculovirus infected silkworm, Bombyx mori. Biol Chem Hoppe Seyler 375:413-418
16. Rosenblat M, Vayab J, Shihc D, Avirama M (2005) Paraoxonase 1 (PON1) enhances HDL-mediated macrophage cholesterol efflux via the ABCA1 transporter in association with increased HDL binding to the cells: a possible role for lysophosphatidylcholine. Atherosclerosis 179:69-77
17. Shi X, Qin J, Zhu J, Zhu D (1996) Expression of biologically active human granulocyte-macrophage colony-stimulating factor in the silkworm (Bombyx mori). Biotechnol Appl Biochem 24:245-249
18. Shih DM, Gu L, Xia YR, Navab M, Li WF, Hama S, Castellani LW, Furlong CE, Costa LG, Fogelman AM, Lusis AJ (1998) Mice lacking serum paraoxonase are susceptible to organophosphate toxicity and atherosclerosis. Nature 394:284-287
19. Sorenson RC, Bisagaier CL, Aviram M, Hsu C, La Du BN (1999) Human serum paraoxonase/arylesterase's retained hydrophobic N-terminal leader sequence associates with HDLs by binding phospholipids: apolipoprotein A-I stabilizes activity. Arterioscler Thromb Vasc Biol 19:2214-2225
20. Vitarius JA, Sultatos LG (1995) The role of calcium in the hydrolysis of the organophosphate paraoxon by human serum A-esterase. Life Sci 56:125-134
21. Wang L, Qin J, Shen B, Zhu J, Zang Y (1995) Expression of human stem cell factor in the baculoviruses expression vector system. Biochem Mol Biol Int 37:729-736