Statistical determination of threshold for cellular division in the CFSE-labeling assay

Journal of Immunological Methods

Volumn 312, Issue 1-2, 2006, Pages 126-136

  Liu, Dacheng ^a   Yu, Jie ^a   Chen, Huiyuan ^a   Reichman, Richard ^a   Wu, Hulin ^a   Jin, Xia ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

Cell division; Cell proliferation; CFSE; Flow Cytometry (FACS); Threshold

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER;

ARTICLE; CALCULATION; CELL DIVISION; CELL LABELING; CELL PROLIFERATION; COMPUTER PROGRAM; CONFIDENCE INTERVAL; HUMAN; HUMAN CELL; LABORATORY; NORMAL HUMAN; PRIORITY JOURNAL; STATISTICAL ANALYSIS; STIMULATION;

CELL DIVISION; CELL PROLIFERATION; DATA INTERPRETATION, STATISTICAL; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; HUMANS; LEUKOCYTES, MONONUCLEAR; STAINING AND LABELING; SUCCINIMIDES;

EID: 33747070280     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.03.010     Document Type: Article

References (15)

1. Appay V., Dunbar P.R., Little S., Havlir D.V., Richman D.D., Gruener N., Pape G., Waters A., Easterbrook P., Salio M., Cerundolo V., McMichael A.J., and Rowland-Jones SL. Memory CD8+ T cells vary in differentiation phenotype in different persistent virus infections. Nat. Med. 8 4 (2002) 379
2. Champagne P., Ogg G.S., King A.S., Knabenhans C., Ellefsen K., Nobile M., Appay V., Rizzardi G.P., Fleury S., Lipp M., Forster R., Rowland_Jones S., Sekaly R.P., McMichael A.J., and Pantaleo G. Skewed maturation of memory HIV-specific CD8 T lymphocytes. Nature 410 (2001) 106
3. Gett A.V., and Hodgkin P.D. A cellular calculus for signal integration by T cells. Nat. Immunol. 1 (2000) 239
4. Gett A.V., Sallusto F., Lanzavecchia A., and Geginat J. T cell fitness determined by signal strength. Nat. Immunol. 4 (2003) 355
5. Givan A.L., Fisher J.L., Waugh M., Ernstoff M.S., and Wallace P.K. A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens. J. Immunol. Methods 230 (1999) 99
6. Hasbold J., Gett A.V., Rush J.S., Deenick E., Avery D., Jun J., and Hodgkin P.D. Quantitative analysis of lymphocyte differentiation and proliferation in vitro using carboxyfluorescein diacetate succinimidyl ester. Immunol. Cell Biol. 77 6 (1999) 516
7. Lichterfeld M., Kaufmann D.E., Yu X.G., Mui S.K., Addo M.M., Johnston M.N., Cohen D., Robbins G.K., Pae E., Alter G., Wurcel A., Stone D., Rosenberg E.S., Walker B.D., and Altfeld M. Loss of HIV-1-specific CD8+ T cell proliferation after acute HIV-1 Infection and restoration by vaccine-induced HIV-1-specific CD4+ T cells. J. Exp. Med. 200 (2004) 701
8. Lyons A.B., Hasbold J., and Hodgkin P.D. Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stably integrated fluorescent probe. Methods Cell Biol. 63 (2001) 375
9. Migueles S.A., Laborico A.C., Shupert W.L., Sabbaghian M.S., Rabin R., Hallahan C.W., Van_Baarle D., Kostense S., Miedema F., McLaughlin M., Ehler L., Metcalf J., Liu S., and Connors M. HIV-specific CD8+ T cell proliferation is coupled to perforin expression and is maintained in nonprogressors. Nat. Immunol. 3 (2002) 1061
10. Papagno L., Spina C.A., Marchant A., Salio M., Rufer N., Little S., Dong T., Chesney G., Waters A., Easterbrook P., Dunbar P.R., Shepherd D., Cerundolo V., Emery V., Griffiths P., Conlon C., McMichael A.J., Richman D.D., Rowland-Jones S.L., and Appay V. Immune activation and CD8+ T-cell differentiation towards senescence in HIV-1 infection. PLoS Biol. 2 2 (2004) E20
11. Seamer L.C., Bagwell C.B., Barden L., Redelman D., Salzman G.C., Wood J.C.S., and Murphy R.F. Proposed new data file standard for flow cytometry, version FCS 3.0. Cytometry 28 (1997) 118
12. Wells A.D., Gudmundsdottir H., and Turka L.A. Following the fate of individual T cells throughout activation and clonal expansion. Signals from T cell receptor and CD28 differentially regulate the induction and duration of a proliferative response. J. Clin. Invest. 100 12 (1997) 3173
13. Wherry E.J., Teichgraber V., Becker T.C., Masopust D., Kaech S.M., Antia R., von_Andrian U.H., and Ahmed R. Lineage relationship and protective immunity of memory CD8 T cell subsets. Nat. Immunol. 4 (2003) 225
14. Wherry E.J., and Ahmed R. Memory CD8 T-cell differentiation during viral infection. J. Virol. 78 11 (2004) 5535
15. Younes S.A., Yassine-Diab B., Dumont A.R., Boulassel M.R., Grossman Z., Routy J.P., and Sekaly RP. HIV-1 viremia prevents the establishment of interleukin 2-producing HIV-specific memory CD4+ T cells endowed with proliferative capacity. J. Exp. Med. 198 12 (2003) 1909