Increased protein yields from Escherichia coli using pressure-cycling technology

Journal of Biomolecular Techniques

Volumn 17, Issue 2, 2006, Pages 173-175

  Smejkal, Gary B ^a   Robinson, Myra H ^b   Lawrence, Nathan P ^a   Tao, Feng ^a   Saravis, Calvin A ^a   Schumacher, Richard T ^a  

^a Pressure BioSciences   (United States)

^b Proteome Systems   (United States)

Author keywords

Escherichia coli; Isoelectric focusing; Pressure cycling technology; Proteins; Proteomics; Two dimensional gel electrophoresis

Indexed keywords

ESCHERICHIA COLI PROTEIN;

ARTICLE; BIOTECHNOLOGY; CHEMISTRY; ESCHERICHIA COLI; IMAGE PROCESSING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; OSCILLOMETRY; PRESSURE; PROTEOMICS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BIOTECHNOLOGY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; IMAGE PROCESSING, COMPUTER-ASSISTED; OSCILLOMETRY; PRESSURE; PROTEOMICS;

EID: 33747051801     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

1. U.S. Department of Labor, Occupational Safety and Health Administration. Workplace precautions against West Nile virus. Safety and Health Information Bulletin 2003, SHIB 03-08-29 (B).
2. Schumacher RT, Manak M, Garrett P, Miller W, Lawrence N, Tao F. Automated solution for sample preparation: Nucleic acid and protein extraction from cells and tissues using pressure cycling technology (PCT). Am Laboratory 2002;34:38-43.
3. Rabilloud T, Blisnick T, Heller M, Luche S, Aebersold R, Lunardi J, Braun-Breton C. Analysis of membrane proteins by two-dimensional electrophoresis: Comparison of the proteins extracted from normal or Plasmodium falciparum-infected erythrocyte ghosts. Electrophoresis 1999;20:3603-3610.
4. Smejkal GB, Lazarev A. Solution phase isoelectric fractionation in the multi-compartment electrolyser: A divide and conquer strategy for the analysis of complex proteomes. Briefings Funct Genomics Proteomics 2005;4:76-81.