A fast mutagenesis procedure to recover soluble and functional scFvs containing amber stop codons from synthetic and semisynthetic antibody libraries

Journal of Immunological Methods

Volumn 312, Issue 1-2, 2006, Pages 182-189

  Barderas, Rodrigo ^a   Shochat, Susana ^b   Martínez Torrecuadrada, Jorge ^a   Altschuh, Danièle ^b   Meloen, Rob ^c   Ignacio Casal, Jose ^a  

^a Centro Nacional de Investigaciones Oncológicas   (Spain)

^b UNIVERSITÉ DE STRASBOURG   (France)

^c Pepscan Systems   (Netherlands)

Author keywords

Affinity determinations; Mutagenesis; Phage display; Recombinant antibodies; scFv

Indexed keywords

GASTRIN; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

AMBER MUTANT; ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; ESCHERICHIA COLI; GENE FUNCTION; GENE FUSION; GENE LIBRARY; GENE SEQUENCE; HUMAN; MUTAGENESIS; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR BINDING; SITE DIRECTED MUTAGENESIS; STOP CODON;

AMINO ACID SEQUENCE; ANTIBODIES; CODON, TERMINATOR; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GASTRINS; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SURFACE PLASMON RESONANCE; VIRAL FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 33747038144     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.03.005     Document Type: Article

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