Purification and characterization of trypsin from the spleen of tongol tuna (Thunnus tonggol)

Journal of Agricultural and Food Chemistry

Volumn 54, Issue 15, 2006, Pages 5617-5622

  Klomklao, Sappasith ^a   Benjakul, Soottawat ^a   Visessanguan, Wonnop ^b   Kishimura, Hideki ^c   Simpson, Benjamin K ^d  

^a PRINCE OF SONGKLA UNIVERSITY   (Thailand)

^b NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^c HOKKAIDO UNIVERSITY   (Japan)

^d MCGILL UNIVERSITY   (Canada)

Author keywords

N terminal amino acid sequence; Proteinase; Purification; Trypsin; Tuna; Viscera

Indexed keywords

TRYPSIN; TRYPSIN INHIBITOR;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; PH; SPLEEN; TEMPERATURE; TUNA;

AMINO ACID SEQUENCE; ANIMALS; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SPLEEN; TEMPERATURE; TRYPSIN; TRYPSIN INHIBITORS; TUNA;

GLYCINE MAX; SCOMBRIDAE GEN. SP.; THUNNUS TONGGOL;

EID: 33746894078     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf060699d     Document Type: Article

References (22)

1. Klomklao, S.; Benjakul, S.; Visessanguan, W. Comparative studies on proteolytic activity of spleen extracts from three tuna species commonly used in Thailand. J. Food Biochem. 2004, 28, 355-372.
2. Klomklao, S.; Benjakul, S.; Visessanguan, W.; Simpson, B. K.; Kishimura, H. Partitioning and recovery of proteinase from tuna spleen by aqueous two-phase systems. Proc. Biochem. 2005, 40, 3061-3067.
3. Kim, H. R.; Meyers, S. P.; Pyeun, J. H.; Godber, J. S. Enzymatic properties of anionic trypsins from the hepatopancreas of crayfish, Procambarus clarkia. Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol. 1994, 107, 197-203.
4. Bezerra, R. S.; Santo, J. F.; Paiva, P. M. G.; Correia, M. T. S.; Coelho, L. C. B. B.; Vieira, V. L. A.; Carvalho, L. B. Partial purification and characterization of a thermostable trypsin from pyloric caeca of tambaqui (Colossoma macropomum). J. Food Biochem. 2001, 25, 199-210.
5. Kishimura, H.; Hayashi, K.; Miyashita, Y.; Nonami, Y. Characteristics of trypsins from the viscera of true sardine (Sardinops melanostictus) and the pyloric caeca of arabesque greenling (Pleuroprammus azonus). Food Chem. 2006, 97, 65-70.
6. Kishimura, H.; Hayashi, K.; Miyashita, Y.; Nonami, Y. Characteristics of two trypsin isozymes from the viscera of Japanese anchovy (Engraulis japonica). J. Food Biochem. 2005, 29, 459-469.
7. Klomklao, S.; Benjakul, S.; Visessanguan, W.; Kishimura, H.; Simpson, B. K. Purification and characterization of trypsins from the spleen of skipjack tuna (Katsuwonus pelamis). Food Chem. 2006, in press.
8. Klomklao, S.; Benjakul, S.; Visessanguan, W.; Kishimura, H.; Simpson, B. K.; Saeki, H. Trypsins from yellowfin tuna (Thunnus albacores) spleen: Purification and characterization. Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol. 2006, 144, 47-56.
9. Hummel, B. C. W. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can. J. Biochem. Phys. 1959, 37, 1393-1399.
10. 4. Nature 1970, 277, 680-685.
11. Lineweaver, H.; Burk, D. The determination of enzyme dissociation constants. J. Am. Chem. Soc. 1934, 56, 665-666.
12. Lowry, O. H.; Rosebrough, N. J.; Fan, A. L.; Randall, R. J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 1951, 193, 256-275.
13. Kristjansson, M. Purification and characterization of trypsin from the pyloric caeca of rainbow trout (Oncorhynchus mykiss). J. Agric. Food Chem. 1991, 39, 1738-1742.
14. Yoshinaka, R.; Sato, M.; Suzuki, T.; Ikeda, S. Purification and some properties of two anionic trypsins from the eel (Anguilla japonica). Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol. 1985, 80, 5-9.
15. Benjakul, S.; Morrissey, M. T. Protein hydrolysates from Pacific whiting solid wastes. J. Agric. Food Chem. 1997, 45, 3423-3430.
16. Simpson, B. K. Digestive proteinases from marine animals. In Seafood Enzymes: Utilization and Influence on Postharvest Seafood Quality; Haard, N. F., Simpson, B. K., Eds.; Marcel Dekker: New York, 2000; pp 531-540.
17. Gudmundsdottir, A.; Gudmundsdottir, E.; Oskarsson, S.; Bjanrnason, J. B.; Eakin, A. K.; Craik, C. S. Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen. Eur. J. Biochem. 1993, 217, 1091-1097.
18. Male, R.; Lorens, L. B.; Smalas, A. O.; Torrissen, K. R. Molecular cloning and characterization of anionic and cationic variants of trypsin from Atlantic salmon. Eur. J. Biochem. 1995, 232, 677-685.
19. Titani, K.; Ericsson, L. H.; Neurath, H.; Walsh, K. A. Amino acid sequence of dogfish trypsin. Biochemistry 1975, 14, 1358-1366.
20. Pinsky, S. D.; Laforge, K. S.; Scheele, G. Differential regulation of trypsinogen mRNA translation: Full-length mRNA sequences encoding two oppositely charged trypsinogen isoenzymes in the dog pancreas. Mol. Cell. Biol. 1985, 5, 2669-2676.
21. Hermodson, M. A.; Ericsson, L. H.; Neurath, H.; Walsh, K. A. Determination of the amino sequence of porcine trypsin by sequenator analysis. Biochemistry 1973, 12, 3146-3153.
22. Walsch, K. A. Trypsinogens and trypsins of various species. Methods Enzymol. 1970, 19, 41-63.