Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients

Journal of Virological Methods

Volumn 135, Issue 2, 2006, Pages 263-268

  Kozić, Sanja ^a   Vince, Adriana ^a   Beš, Janja Iščić ^a   Rode, Oktavija Daković ^a   Lepej, Snježana Židovec ^a   Poljak, Mario ^b   Bozic, Michael ^c   Kessler, Harald H ^c  

^a Croatia   (Croatia)

^b Slovenian AIDS Reference Centre   (Slovenia)

^c MEDICAL UNIVERSITY OF GRAZ   (Austria)

Author keywords

Epstein Barr virus; Immunosupression; Quantitation; Real time PCR; Viral load

Indexed keywords

EDETIC ACID; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; VIRUS DNA;

ADULT; ARTICLE; BLOOD SAMPLING; CHILD; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA EXTRACTION; EPSTEIN BARR VIRUS; HUMAN; INFANT; MAJOR CLINICAL STUDY; ORGAN TRANSPLANTATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; STATISTICAL SIGNIFICANCE; VIRUS INFECTION;

DNA, VIRAL; HERPESVIRUS 4, HUMAN; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC;

HUMAN HERPESVIRUS 4;

EID: 33746881028     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.03.013     Document Type: Article

References (25)

1. Andreone P., Gramenzi A., Lorenzini S., Biselli M., Cursaro C., Pileri S., and Bernardi M. Posttransplantation lymphoproliferative disorders. Arch. Intern. Med. 163 (2003) 1997-2004
2. Auer M., Espy M., Smith T.F., Oberlander U., and Haberhausen G. Development of a quantitative LightCycler assay for the detection of Epstien-Barr virus DNA in research samples. Biochemica 3 (2002) 6-8
3. Bai X., Hosler G., Barton Rogers B., Dawson D.B., and Scheuermann R.H. Quantitative polymerase chain reaction for human herpesvirus diagnosis and measurement of Epstein-Barr virus burden in posttransplant lymphoproliferative disorder. Clin. Chem. 43 (1997) 1843-1849
4. Baldanti F., Grossi P., Furione M., Simoncini L., Sarasini A., and Comoli P. High levels of Epstein-Barr virus in blood of solid-organ transplant recipients and their value in predicting posttransplant lymphoproliferative disorders. J. Clin. Microbiol. 38 (2000) 613-619
5. Brengel-Pesce K., Morand P., Schmuck A., Bourgeat M.-J., Buisson M., Bargues G., Bouzid M., and Seigneurin J.M. Routine use of real-time quantitative PCR for laboratory diagnosis of Epstein-Barr virus infections. J. Med. Virol. 66 (2002) 360-369
6. Cohen J.I. Epstein-Barr virus infection. N. Engl. J. Med. 343 (2000) 481-492
7. Fafi-Kremer S., Brengel-Pesce K., Bargues G., Bourgeat M.J., Seigneurin J.M., and Morand P. Assessment of automated DNA extraction coupled with real-time PCR for measuring Epstein-Barr virus load in whole blood, peripheral mononuclear cells and plasma. J. Clin. Virol. 30 (2004) 157-164
8. Jebbink J., Bai X., Barton Rogers B., Dawson D.B., Sheuermann R.H., and Saad R.D. Development of real-time PCR assays for the quantitative detection of Epstein-Barr virus and cytomegalovirus, comparison of TaqMan probes, and molecular beacons. J. Mol. Diagn. 5 (2003) 15-20
9. Johannsen E.C., Schooley R.T., and Kaye K.M. Epstein-Barr virus (infectious mononucleosis). Principles and Practice of Infectious Diseases. 6th ed. (2005), Churchill-Livingstone, Philadelphia (PA) pp. 1802-1803
10. Kessler H.H., Stelzl E., Daghofer E., Santner B.I., Marth E., Lackner H., and Stauber R.E. Semiautomated quantification of hepatitis B virus DNA in a routine diagnostic laboratory. Clin. Diagn. Lab. Immunol. 7 (2000) 853-855
11. Kimura H., Morita M., Yabuta Y., Kuzushima K., Kato K., Kojima S., Matsuyama T., and Morishima T. Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J. Clin. Microbiol. 37 (1999) 132-136
12. Limaye A.P., Huang M., Atienza E.E., Ferrenberg J.M., and Corey L. Detection of Epstein-Barr virus DNA in sera from transplant recipients with lymphoproliferative disorders. J. Clin. Microbiol. 37 (1999) 1113-1116
13. Meerbach A., Gruhn B., Egerer R., Reischl U., Zintl F., and Wutzler P. Semiquantitative PCR Analysis of Epstein-Barr virus DNA in clinical samples of patients with EBV-associated diseases. J. Med. Virol. 65 (2001) 348-357
14. Niesters H.G.M., van Esser J., Fries E., Wolthers K.C., Cornelissen J., and Osterhaus A.D.M.E. Development of real-time quantitative assay for detection of Epstein-Barr virus. J. Clin. Microbiol. 38 (2000) 712-715
15. Niesters H.G.M. Summary of Results QCMD 2002 Epstein Barr virus proficiency programme (2003), Quality Control for Molecular Diagnostics, Glasgow, United Kingdom
16. Riddler S.A., Breinig M.C., and McKnight J.L.C. Increased levels of circulating Epstein-Barr virus (EBV)-infected lymphocytes and decreased EBV nuclear antigen antibody responses are associated with the development of posttransplant lymphoprolypherative disease in solid-organ transplant recipients. Blood 84 (1994) 972-984
17. Rowe D.T., Qu L., Reyes J., Jabbour N., Yunis E., Putnam P., Todo T., and Green M. Use of quantitative competitive PCR To measure Epstein-Barr virus genome load in the peripheral blood of pediatric transplant patients with lymphoproliferative disorders. J. Clin. Microbiol. 35 (1997) 1612-1615
18. Stelzl E., Muller Z., Marth E., and Kessler H.H. Rapid quantification of hepatitis B virus DNA by automated sample preparation and real-time PCR. J. Clin. Microbiol. 42 (2004) 2445-2449
19. Stevens S.J.C., Verschuuren E.A.M., Pronk I., van der Bij W., Harmsen M.C., The T.H., Meijer C.J.L.M., van den Brule A.J.C., and Middeldorp J.M. Frequent monitoring of Epstein-Barr virus DNA load in unfractionated whole blood is essential for early detection of posttransplant lymphoproliferative disease in high-risk patients. Blood 97 (2001) 1165-1171
20. Stevens S.J.C., Pronk I., and Middeldorp J.M. Toward standardization of Epstein-Barr virus DNA load monitoring: unfractionated whole blood as preferred clinical specimen. J. Clin. Microbiol. (2001) 1211-1216
21. Stevens S.J.C., Blank B.S.N., Smits P.H.M., Meenhorst P.L., and Middeldorp J.M. High Epstein-Barr virus (EBV) DNA loads in HIV-infected patients: correlation with antiretroviral therapy and quantitative EBV serology. AIDS 16 (2002) 993-1001
22. Stevens S.J.C., Verschuuren E.A.M., Verkuijlen A.W.M., van den Brule A.J.C., Meijer C.J.L.M., and Middeldorp J.M. Role of the Epstein-Barr virus DNA load monitoring in prevention and early detection of post-transplant lymphoproliferative disease. Leuk. Lymphoma 43 (2002) 831-840
23. Stevens S.J.C., Brink A.A.T.P., and Middeldorp J.M. Profiling of Epstein-Barr virus latent RNA expression in clinical specimen by gene-specific multiprimed cDNA synthesis and PCR. Methods Mol. Biol. 292 (2005) 27-38
24. van Esser J.W.J., van der Holt B., Meijer E., Niesters H.G.M., Trenschel R., Thijsen S.F.T., van Loon A.M., Frassoni F., Bacigalupo A., Schaefer U.W., Osterhaus A.D.M.E., Gratama J.W., Lowenberg B., Verdonck L.F., and Cornelissen J.J. Epstein-Barr virus (EBV) reactivation is a frequent event after allogenic stem-cell transplantation (SCT) and quantitatively predicts EBV-lymphoproliferative disease following T-cell depleted SCT. Blood 98 (2001) 972-978
25. Verschuuren E., van der Bij W., de Boer W., Timens W., Middeldorp J., and The T.H. Quantitative Epstein-Barr virus (EBV) serology in lung transplant recipients with primary EBV infection and/or post-transplant lymphoproliferative disease. J. Med. Virol. 69 (2003) 258-266